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Rabbit Polyclonal Monoamine Oxidase B/MAOB antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MAOB aa 1-200.

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Images

Western blot - Anti-Monoamine Oxidase B/MAOB antibody (AB137863), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody (AB137863), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Monoamine Oxidase B/MAOB antibody (AB137863), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human MAOB aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P27338

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Cow
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Cow, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Predicted
Predicted

Species

Cow, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat mediated antigen retrieval before commencing with IHC staining protocol using10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Predicted
Predicted

Species

Cow, Pig

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Catalyzes the oxidative deamination of primary and some secondary amines such as neurotransmitters, and exogenous amines including the tertiary amine, neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), with concomitant reduction of oxygen to hydrogen peroxide and participates in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues (PubMed:11049757, PubMed:11134050, PubMed:20493079, PubMed:8316221, PubMed:8665924). Preferentially degrades benzylamine and phenylethylamine (PubMed:11049757, PubMed:11134050, PubMed:20493079, PubMed:8316221, PubMed:8665924).

Alternative names

Recommended products

Rabbit Polyclonal Monoamine Oxidase B/MAOB antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MAOB aa 1-200.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human MAOB aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P27338
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Monoamine oxidase B (MAOB) also known as MAO-B or MOA-B is a flavoenzyme that catalyzes the oxidative deamination of amines. It plays an important role in the catabolism of monoamines including neurotransmitters such as dopamine. The enzyme is approximately 58 kDa in size and is primarily expressed in the brain particularly in the basal ganglia region but also found in platelets and other tissues. MAOB exists in the outer mitochondrial membrane where it is closely associated with cellular respiration processes.

Biological function summary

Monoamine oxidase B aids in the regulation of neurotransmitter levels by breaking down dopamine phenylethylamine and other bioamines. It acts individually rather than forming part of larger protein complexes. This regulatory action influences several neurological processes contributing to the synaptic transmission and maintaining homeostasis in central nervous system functions. The activity of MAOB varies with age generally increasing over time which has implications for its role in aging-related neural changes.

Pathways

Monoamine oxidase B is involved in the dopamine metabolic pathway which impacts neurological functions and behaviors. It also connects to the phenylethylamine catabolic process influencing neuropsychiatric states. MAOB directly interacts with proteins like dopamine transporter proteins that influence dopamine reuptake and it works alongside monoamine oxidase A (MAOA) which degrades other neurotransmitters such as serotonin and norepinephrine providing a comprehensive regulatory mechanism within the monoamine system.

Associated diseases and disorders

Monoamine oxidase B has a significant connection to neurodegenerative diseases such as Parkinson's disease and psychiatric disorders like depression. Its increased activity is linked to the neurodegeneration observed in Parkinson's due to excessive breakdown of dopamine. Through the process of oxidative stress MAOB contributes to neuronal damage particularly when interacting with proteins like α-synuclein aggravating the disease pathology. Additionally its regulation of dopamine levels can influence depressive states indicating potential therapeutic targets for MAOB inhibitors in these conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody (ab137863), expandable thumbnail

    Western blot - Anti-Monoamine Oxidase B/MAOB antibody (ab137863)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-Monoamine Oxidase B/MAOB antibody (ab137863) at 1/3000 dilution

    All lanes: HepG2 whole cell lysate at 30 µg

    Predicted band size: 59 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody (ab137863), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody (ab137863)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling using Monoamine Oxidase B/MAOB with ab137863 at 1/250 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Monoamine Oxidase B/MAOB antibody (ab137863), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Monoamine Oxidase B/MAOB antibody (ab137863)

    Immunofluorescence analysis of methanol fixed HepG2 cells labelling Monoamine Oxidase B/MAOB with ab137863 at 1/200 dilution. The lower image shows costaining with Hoechst 33342.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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