Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] - BSA and Azide free (AB279712)

This data was developed using ab259928, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Monoamine Oxidase B with ab259928 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasm staining on mouse liver. The section was incubated with ab259928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] - BSA and Azide free (AB279712)

This data was developed using ab259928, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Monoamine Oxidase B with ab259928 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasm staining on rat liver. The section was incubated with ab259928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Lab

Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] - BSA and Azide free (AB279712)

This data was developed using ab259928, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile observed is consistent with what has been described in the literature (PMID : 8058968).

Low expression : mouse testis.

Protein aggregates may be caused by boiling of samples .

Exposure time : 15 seconds

All lanes:

Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (<a href='/en-us/products/primary-antibodies/monoamine-oxidase-b-maob-antibody-epr24131-79-ab259928'>ab259928</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 59 kDa

Observed band size: 59 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] - BSA and Azide free (AB279712)

This data was developed using ab259928, the same antibody clone in a different buffer formulation.

Monoamine Oxidase B was immunoprecipitated from 0.35 mg Mouse liver tissue lysate 10 ug with ab259928 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259928 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse liver tissue lysate 10 ug

Lane 2 : ab259928 IP in Mouse liver tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259928 in mouse liver tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds

Sample loaded onto lane 1 are non-boiled as boiling may cause protein aggregates.

All lanes:

Immunoprecipitation - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (<a href='/en-us/products/primary-antibodies/monoamine-oxidase-b-maob-antibody-epr24131-79-ab259928'>ab259928</a>)

Predicted band size: 59 kDa

Observed band size: 59 kDa

false

• WB

Lab

Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] - BSA and Azide free (AB279712)

This data was developed using ab259928, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : 15 seconds

All lanes:

Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (<a href='/en-us/products/primary-antibodies/monoamine-oxidase-b-maob-antibody-epr24131-79-ab259928'>ab259928</a>) at 1/1000 dilution

Lane 1:

E.coli extracts containing his-tagged mouse Amine oxidase flavin-containi B recombinant protein (aa 2-489) at 10 µL

Lane 2:

E.coli extracts containing his-tagged mouse Amine oxidase flavin-containi A recombinant protein (aa 1-497) at 4 µL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 59 kDa

false