Rabbit Polyclonal MORC3 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MORC3 aa 150-300.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MORC3 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q14149
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Nuclear matrix protein which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism and plays a role in innate immunity by restricting different viruses through modulation of the IFN response (PubMed:27440897, PubMed:34759314). Mechanistically, possesses a primary antiviral function through a MORC3-regulated element that activates IFNB1, and this function is guarded by a secondary IFN-repressing function (PubMed:34759314). Sumoylated MORC3-NBs associates with PML-NBs and recruits TP53 and SP100, thus regulating TP53 activity (PubMed:17332504, PubMed:20501696). Binds RNA in vitro (PubMed:11927593). Histone methylation reader which binds to non-methylated (H3K4me0), monomethylated (H3K4me1), dimethylated (H3K4me2) and trimethylated (H3K4me3) 'Lys-4' on histone H3 (PubMed:26933034). The order of binding preference is H3K4me3 > H3K4me2 > H3K4me1 > H3K4me0 (PubMed:26933034).(Microbial infection) May be required for influenza A transcription during viral infection (PubMed:26202233).
Alternative names
KIAA0136, NXP2, ZCWCC3, MORC3, MORC family CW-type zinc finger protein 3, Nuclear matrix protein 2, Zinc finger CW-type coiled-coil domain protein 3
Recommended products
Rabbit Polyclonal MORC3 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MORC3 aa 150-300.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MORC3 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q14149
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MORC3 also known as Microrchidia CW-type zinc finger protein 3 is a nuclear protein with a molecular weight of approximately 100 kDa. It functions as part of chromatin remodeling processes and is found within the nucleus of cells. MORC3 exhibits a high level of expression in testes and other tissues supporting its role in transcription regulation and nuclear body formation. It also contains several domains such as ATPase zinc finger and a nuclear localization signal which are critical for its mechanical action within the cell.
- Biological function summary
MORC3 influences nuclear dynamics and chromatin organization. It plays a central role in the formation of promyelocytic leukemia nuclear bodies (PML-NBs) which are involved in gene regulation DNA repair and apoptosis. MORC3 can interact with proteins within PML-NBs indicating it’s part of a protein complex that maintains genomic stability. This organizational role supports various cellular processes by regulating gene expression patterns in response to stress and damage.
- Pathways
MORC3 integrates into different signaling pathways including DNA damage response and gene repression-specific pathways. It participates in the p53 signaling pathway where its interaction with proteins like p300 influences transcriptional activation of p53 target genes. Moreover MORC3's involvement in these pathways highlights processes such as cell cycle regulation apoptosis and maintenance of genomic integrity under stress conditions.
- Associated diseases and disorders
MORC3 has been associated with autoimmune conditions and cancer. Variants or dysregulation in MORC3 expression can relate to systemic lupus erythematosus where it impacts immune responses through proteins like TRIM21 known for its role in autoimmunity. Additionally changes in MORC3 expression levels have correlations with certain cancer types where aberrant nuclear body formation affects cell proliferation and survival mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-MORC3 antibody (ab222086)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MORC3 (green) using ab222086 at 4 μg/ml in ICC/IF.
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