AB171937

Anti-MOS antibody [EPR6720(2)-23]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal MOS antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

Proto-oncogene serine/threonine-protein kinase mos, Oocyte maturation factor mos, Proto-oncogene c-Mos, MOS

2 Images

Flow Cytometry (Intracellular) - Anti-MOS antibody [EPR6720(2)-23] (AB171937)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MOS antibody [EPR6720(2)-23] (AB171937)

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling MOS with ab171937 at 1/100 (red) or a rabbit IgG (negative) (green).

Supplier Data

Western blot - Anti-MOS antibody [EPR6720(2)-23] (AB171937)

All lanes:

Western blot - Anti-MOS antibody [EPR6720(2)-23] (ab171937) at 1/1000 dilution

Lane 1:

Jurkat cell line lysate at 10 µg

Lane 2:

293T cell line lysate at 10 µg

Lane 3:

Mouse testis tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/500 dilution

Predicted band size: 38 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6720(2)-23

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MOS protein also known simply as MOS is a serine/threonine-protein kinase with a mass of approximately 39 kDa. It is particularly expressed in oocytes and is an essential component during oocyte maturation. MOS acts mechanically by regulating the transition from meiotic prophase to metaphase and ensures the metaphase arrest which is important for successful fertilization. It is well-conserved across species including in the commonly studied 'mos mouse' model which facilitates detailed exploration of its functions.

Biological function summary

The MOS protein plays a significant role in the regulation of the meiotic cell cycle. It is part of the Mos-MAPK pathway where it activates MAP kinase (MAPK) to propagate signals that stabilize cell division processes in oocytes. MOS ensures the correct timing of progression through meiosis impacting the fidelity and viability of the mature eggs. It forms a complex with other proteins such as MAPK and MEK for effective signal transduction during oocyte maturation.

Pathways

MOS integrates into the mitogen-activated protein kinase (MAPK) signaling pathway which is pivotal for cell cycle regulation in oocytes. The protein is closely related to MEK1 and MAPK which together construct the MAPK cascade leading to activation of key meiotic processes. This cascade also influences the stabilization of cyclin B-CDK1 another intricate player in cell cycle control linking MOS to broader regulatory networks in cell cycle checkpoint regulation.

MOS shows potential involvement in conditions related to infertility and reproductive dysfunction. Aberrant expression or mutation of MOS can disrupt normal meiotic processes leading to failed oocyte maturation and infertility. The protein is also connected to disorders involving inappropriate MAPK pathway activation which can lead to oncogenesis. In particular abnormal interaction with proteins like MEK1 and MAPK may contribute to the pathophysiology of certain cancers where dysregulation of cell division pathways is a hallmark.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine kinase involved in the regulation of MAPK signaling. Is an activator of the ERK1/2 signaling cascade playing an essential role in the stimulation of oocyte maturation.

See full target information Proto-oncogene serine/threonine-protein kinase mos

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 80:372 PubMed38001238

2023

Cordycepin delays postovulatory aging of oocytes through inhibition of maternal mRNAs degradation via DCP1A polyadenylation suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Li,Ling Zhu,Jun-Xia Liu,Jing Guo,Juan Xie,Chun-Meng Shi,Qing-Yuan Sun,Guo-Ning Huang,Jing-Yu Li

Scientific reports 13:270 PubMed36609526

2023

Expression of LIM domain-binding 3 (LDB3), a striated muscle Z-band alternatively spliced PDZ-motif protein in the nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Yotam Blech-Hermoni,Kalpana Subedi,Maya Silver,Leah Jensen,Stephen Coscia,Malcolm M Kates,Yongmei Zhao,Castle Raley,Nancy Edwards,Bao Tran,Abhik Ray-Chaudhary,Pankaj Pathak,Ami Mankodi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MOS antibody [EPR6720(2)-23]

Flow Cytometry (Intracellular) - Anti-MOS antibody [EPR6720(2)-23] (AB171937)

Western blot - Anti-MOS antibody [EPR6720(2)-23] (AB171937)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise