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AB323368

Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4]

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Mouse Recombinant Monoclonal NCAP antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Crimean-Congo hemorrhagic fever virus (isolate C68031), Recombinant fragment - Crimean-Congo hemorrhagic fever virus (isolate C68031) samples.

View Alternative Names

Nucleoprotein, Nucleocapsid protein, Protein N

3 Images
Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] (AB323368)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] (AB323368)

Indirect ELISA analysis of ab323368 at 2000 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution dilution.

Antigen : Lysate of 293T cells transfected with a Crimean-Congo hemorrhagic fever virus Nucleoprotein, Dugbe virus (isolate ArD44313) Nucleoprotein, Hazara virus (isolate JC280) Nucleoprotein expression vector containing a myc-His-tag.

Antigen concentration : 1000-0 ug/ml

Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] (AB323368)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] (AB323368)

Indirect ELISA analysis of ab323368 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : recombinant Crimean-Congo hemorrhagic fever virus Nucleoprotein.

Antigen concentration : 5000 ng/ml

Western blot - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] (AB323368)
  • WB

Supplier Data

Western blot - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] (AB323368)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] (ab323368) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containi a myc-His-tag® whole cell lysate at 4 µg

Lane 2:

293T cells transfected with a Crimean-Coo hemorrhagic fever virus Nucleoprotein expression vector containi a myc-His-tag® whole cell lysate at 4 µg

Lane 3:

293T cells transfected with a Dbe virus (isolate ArD44313) Nucleoprotein expression vector containing a myc-His-tag® whole cell lysate at 4 µg

Lane 4:

293T cells transfected with a Hazara virus (isolate JC280) Nucleoprotein expression vector containi a myc-His-tag® whole cell lysate at 4 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 54 kDa,36 kDa,37-50 kDa

false

Exposure time: 3s

  • Carrier free

    Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A4

Isotype

IgG1

Carrier free

No

Reacts with

Crimean-Congo hemorrhagic fever virus (isolate C68031)

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Crimean-Congo hemorrhagic fever virus (isolate C68031)": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Crimean-Congo hemorrhagic fever virus (isolate C68031)": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "500 ng/mL", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Crimean-Congo hemorrhagic fever virus (isolate C68031)": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "500 ng/mL", "IELISA-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds dsRNA and ssRNA and probably participates in the packaging of viral genome (By similarity). In the dsRNA binding mode, the nucleocapsid protein specifically binds to the vRNA panhandle secondary structure formed at the termini of viral genome (By similarity). Does not discriminate between viral and nonviral RNAs through ssRNA binding mode (By similarity). Displays dsDNA endonuclease activity that is sequence non-specific (By similarity).
See full target information N
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com