JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB324847

Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Crimean-Congo hemorrhagic fever virus (isolate C68031), Recombinant fragment - Crimean-Congo hemorrhagic fever virus (isolate C68031) samples.

View Alternative Names

Nucleoprotein, Nucleocapsid protein, Protein N

3 Images
Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)

This data was developed using ab323368, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab323368 at 2000 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution dilution.

Antigen : Lysate of 293T cells transfected with a Crimean-Congo hemorrhagic fever virus Nucleoprotein, Dugbe virus (isolate ArD44313) Nucleoprotein, Hazara virus (isolate JC280) Nucleoprotein expression vector containing a myc-His-tag.

Antigen concentration : 1000-0 ug/ml

Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)

This data was developed using ab323368, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab323368 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : recombinant Crimean-Congo hemorrhagic fever virus Nucleoprotein.

Antigen concentration : 5000 ng/ml

Western blot - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)
  • WB

Supplier Data

Western blot - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)

This data was developed using ab323368, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] (<a href='/en-us/products/primary-antibodies/mouse-crimean-congo-hemorrhagic-fever-virus-nucleoprotein-antibody-a4-ab323368'>ab323368</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containi a myc-His-tag® whole cell lysate at 4 µg

Lane 2:

293T cells transfected with a Crimean-Coo hemorrhagic fever virus Nucleoprotein expression vector containi a myc-His-tag® whole cell lysate at 4 µg

Lane 3:

293T cells transfected with a Dbe virus (isolate ArD44313) Nucleoprotein expression vector containing a myc-His-tag® whole cell lysate at 4 µg

Lane 4:

293T cells transfected with a Hazara virus (isolate JC280) Nucleoprotein expression vector containi a myc-His-tag® whole cell lysate at 4 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 54 kDa,36 kDa,37-50 kDa

false

Exposure time: 3s

  • Unconjugated

    Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A4

Isotype

IgG1

Carrier free

Yes

Reacts with

Crimean-Congo hemorrhagic fever virus (isolate C68031)

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Crimean-Congo hemorrhagic fever virus (isolate C68031)": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Crimean-Congo hemorrhagic fever virus (isolate C68031)": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Crimean-Congo hemorrhagic fever virus (isolate C68031)": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Product details

ab324847 is the carrier-free version of ab323368.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Binds dsRNA and ssRNA and probably participates in the packaging of viral genome (By similarity). In the dsRNA binding mode, the nucleocapsid protein specifically binds to the vRNA panhandle secondary structure formed at the termini of viral genome (By similarity). Does not discriminate between viral and nonviral RNAs through ssRNA binding mode (By similarity). Displays dsDNA endonuclease activity that is sequence non-specific (By similarity).
See full target information N
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com