Anti-mouse IgG fab antibody [RMG05] (ab197774) is a goat monoclonal antibody that is used to detect mouse IgG fab in ELISA. Suitable for Mouse samples.
- Recombinant format for unrivalled batch-batch consistency
Images
![ELISA - Anti-mouse IgG fab antibody [RMG05] (AB197774), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg-fab-antibody-rmg05-ab197774--elisa-img55678.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-mouse IgG fab antibody [RMG05] (AB197774)")
![ELISA - Anti-mouse IgG fab antibody [RMG05] (AB197774), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg-fab-antibody-rmg05-ab197774--elisa-img73806.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-mouse IgG fab antibody [RMG05] (AB197774)")
Key facts
- Isotype
- IgG1
- Host species
- Goat
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| ELISA | |
|---|---|
| Mouse | Tested |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.05-1 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Anti-mouse IgG fab antibody [RMG05] (ab197774) is a goat monoclonal antibody that is used to detect mouse IgG fab in ELISA. Suitable for Mouse samples.
- Recombinant format for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG1
- Host species
- Goat
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- RMG05
- Purification technique
- Affinity purification Protein G
- Specificity
ab197774 reacts to the Fab region of Mouse IgG, including IgG1, IgG2a, IgG2b, and IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG.
- Concentration
- Purification notes
Purified from an animal origin–free culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-mouse IgG fab antibody [RMG05] (ab197774) is a goat recombinant monoclonal antibody and is validated for use in ELISA in Mouse samples.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![ELISA - Anti-mouse IgG fab antibody [RMG05] (ab197774), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg-fab-antibody-rmg05-ab197774--elisa-img55678.jpg "ELISA - Anti-mouse IgG fab antibody [RMG05] (ab197774)")
ELISA - Anti-mouse IgG fab antibody [RMG05] (ab197774)
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of ab197774 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
![ELISA - Anti-mouse IgG fab antibody [RMG05] (ab197774), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg-fab-antibody-rmg05-ab197774--elisa-img73806.jpg "ELISA - Anti-mouse IgG fab antibody [RMG05] (ab197774)")
ELISA - Anti-mouse IgG fab antibody [RMG05] (ab197774)
ELISA of mouse immunoglobulins shows ab197774 reacted to the Fab region of mouse IgG1, IgG2a, and IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab197774 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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