Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control Suitable for ELISA, ICC/IF, Flow Cyt and reacts with samples. Cited in 32 publications.
Images
![Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190--flow-cytometry-img71209.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190)")
![Immunocytochemistry/ Immunofluorescence - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190--immunocytochemistry-immunofluorescence-img18682.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190)")
![ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190--elisa-img84258.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190)")
![Flow Cytometry (Intracellular) - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190--flow-cytometry-intracellular-img400014.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190)")
Publications
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- Nature communications 14:68012023Cross species systems biology discovers glial DDR2, STOM, and KANK2 as therapeutic targets in progressive supranuclear palsy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhao Min,Xue Wang,Özkan İş,Tulsi A Patel,Junli Gao,Joseph S Reddy,Zachary S Quicksall,Thuy Nguyen,Shu Lin,Frederick Q Tutor-New,Jessica L Chalk,Adriana O Mitchell,Julia E Crook,Peter T Nelson,Linda J Van Eldik,Todd E Golde,Minerva M Carrasquillo,Dennis W Dickson,Ke Zhang,Mariet Allen,Nilüfer Ertekin-TanerPubMed 37919278
- Heliyon 9:e198162023The m6A reader YTHDC1-mediated lncRNA CTBP1-AS2 m6A modification accelerates cholangiocarcinoma progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhe Jin,Yahui LiuPubMed 37809459
- Acta biomaterialia 152:273-2892022Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLekha Shah,Ayşe Latif,Kaye J Williams,Annalisa TirellaPubMed 36087866
- Oxidative medicine and cellular longevity 2022:47226472022SIRT3-Mediated CypD-K166 Deacetylation Alleviates Neuropathic Pain by Improving Mitochondrial Dysfunction and Inhibiting Oxidative Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBinbin Yan,Qiang Liu,Xiaobao Ding,Yuwen Lin,Xiaowei Jiao,Yuqing Wu,Huihui Miao,Chenghua ZhouPubMed 36092157
- European cells & materials 44:43-552022Enthesis: not the same in each localisation - a molecular, histological and biomechanical study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC J Peniche Silva,S A Müller,N Quirk,R E De la Vega,M J Coenen,C H Evans,E R Balmayor,M van GriensvenPubMed 35976149
- International journal of molecular sciences 23:2022Identification of Regulatory Element Containing Functional Genetic Variants Associated with Severe Malaria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamia Nisar,Magali Torres,Alassane Thiam,Bruno Pouvelle,Florian Rosier,Frederic Gallardo,Oumar Ka,Babacar Mbengue,Rokhaya Ndiaye Diallo,Laura Brosseau,Salvatore Spicuglia,Alioune Dieye,Sandrine Marquet,Pascal RihetPubMed 35563239
- Cell death discovery 8:1792022METTL3 stabilizes HDAC5 mRNA in an mA-dependent manner to facilitate malignant proliferation of osteosarcoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenbing Jiang,Zhibing Dai,Junshen Wu,Suzhi Ji,Yachao Sun,Wenpeng YangPubMed 35396379
- American journal of physiology. Lung cellular and molecular physiology 322:L712-L7212022Angiotensin-converting enzyme 2 in peripheral lung club cells modulates the susceptibility to SARS-CoV-2 in chronic obstructive pulmonary disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Peng,Zhao-Ni Wang,Shi-Ying Chen,Ai-Ru Xu,Zhang-Fu Fang,Jing Sun,Zi-Qing Zhou,Xiao-Tao Hou,Lai-Jian Cen,Jian-Juan Ma,Jin-Cun Zhao,Wei-Jie Guan,De-Yun Wang,Nan-Shan ZhongPubMed 35318858
- eLife 10:2021Human Erbb2-induced Erk activity robustly stimulates cycling and functional remodeling of rat and human cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicholas Strash,Sophia DeLuca,Geovanni L Janer Carattini,Soon Chul Heo,Ryne Gorsuch,Nenad BursacPubMed 34665129
- Cell reports 36:1096322021High-grade serous ovarian tumor cells modulate NK cell function to create an immune-tolerant microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVeronica D Gonzalez,Ying-Wen Huang,Antonio Delgado-Gonzalez,Shih-Yu Chen,Kenyi Donoso,Karen Sachs,Andrew J Gentles,Grace M Allard,Kevin S Kolahi,Brooke E Howitt,Ermelinda Porpiglia,Wendy J FantlPubMed 34469729
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1 µg/mL | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1 µg/mL | Notes - |
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Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control Suitable for ELISA, ICC/IF, Flow Cyt and reacts with samples. Cited in 32 publications.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
- Clone number
15-6E10A7
- Purification technique
Precipitation Ammonium Sulphate
- Specificity
This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Notes
This antibody clone is manufactured by Abcam.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody's host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190--flow-cytometry-img71209.jpg "Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)")
Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
![Immunocytochemistry/ Immunofluorescence - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190--immunocytochemistry-immunofluorescence-img18682.jpg "Immunocytochemistry/ Immunofluorescence - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)")
Immunocytochemistry/ Immunofluorescence - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Immunocytochemistry (ICC) experiments with NIH-3T3 (mouse sarcoma), COS7 (monkey kidney fibroblast), H9C2 (rat myoblast) and Hela (human adenocarcinoma) were performed with the IgG1 Isotype Control Antibody (top), no primary antibody negative control (middle), and Anti-MTCO1 antibody [1D6E1A8] ab14705 as a positive control (bottom).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
![ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190--elisa-img84258.jpg "ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)")
ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Fluorescence ELISA.
An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.
![Flow Cytometry (Intracellular) - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190--flow-cytometry-intracellular-img400014.jpg "Flow Cytometry (Intracellular) - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)")
Flow Cytometry (Intracellular) - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
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