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AB221848

Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free

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(4 Publications)

Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free Suitable for ICC/IF, Flow Cyt, ELISA and reacts with samples. Cited in 4 publications.
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Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free (AB221848)
  • Flow Cyt

Unknown

Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free (AB221848)

This flow cytometry data was generated using the same antibody clone under a different buffer format (cat# ab170190).

Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).

An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

15-6E10A7

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Applications

ELISA, Flow Cyt, IHC-P, ICC/IF

applications

Specificity

This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry paraffin embedded sections", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "IHCP-species-checked": "", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
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Product details

ab221848 is a PBS only buffer formulated version of ab170190.

Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody's host species, isotype, and possible conjugation.

Immunogen
Recombinant full length protein. ab221848 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Precipitation Ammonium Sulphate
Storage buffer
pH: 7.5 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 6:23300 PubMed26996236

2016

The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation.

Applications

Flow Cyt

Species

Unspecified reactive species

Yi Cui,Jin Han,Zhifeng Xiao,Tong Chen,Bin Wang,Bing Chen,Sumei Liu,Sufang Han,Yongxiang Fang,Jianshu Wei,Xiujie Wang,Xu Ma,Jianwu Dai

The Journal of biological chemistry 291:1974-90 PubMed26627833

2015

Oxidative Stress Attenuates Lipid Synthesis and Increases Mitochondrial Fatty Acid Oxidation in Hepatoma Cells Infected with Hepatitis C Virus.

Applications

IHC-P

Species

Unspecified reactive species

Donna N Douglas,Christopher Hao Pu,Jamie T Lewis,Rakesh Bhat,Anwar Anwar-Mohamed,Michael Logan,Garry Lund,William R Addison,Richard Lehner,Norman M Kneteman

Cell and tissue banking 17:137-45 PubMed26220398

2015

Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Landa-Solís,Julio Granados-Montiel,Anell Olivos-Meza,Carmina Ortega-Sánchez,Mónica Cruz-Lemini,Cecilia Hernández-Flores,María Eugenia Chang-González,Ricardo Gómez García,Brenda Olivos-Díaz,María Cristina Velasquillo-Martínez,Carlos Pineda,Clemente Ibarra

Veterinary journal (London, England : 1997) 206:191-6 PubMed26364240

2015

Significance of caveolin-1 and matrix metalloproteinase 14 gene expression in canine mammary tumours.

Applications

IF

Species

Unspecified reactive species

M Ebisawa,H Iwano,M Nishikawa,Y Tochigi,T Komatsu,Y Endou,K Hirayama,H Taniyama,T Kadosawa,H Yokota
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
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Alternative Version
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AB178000

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Alternative Version
Primary Antibodies

AB170190

Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com