Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353) is a mouse monoclonal antibody that is used in Flow Cytometry, IHC-P, IHC-Fr.
- Over 40 publications
- Trusted since 2010
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 2 µg for 106 Cells | Notes ab91353 is a negative isotype control |
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Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353) is a mouse monoclonal antibody that is used in Flow Cytometry, IHC-P, IHC-Fr.
- Over 40 publications
- Trusted since 2010
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA
ab91353 is purified and passed through a 0.22µm filter
ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections
Immunogen
Chemical / Small Molecule corresponding to Mouse. synthetic hapten, which is normally not present in humans or animals.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Cell surface expression of IGF1R by FACScan analysis. IGF1R expression level of Panc-1, RWP-1, OCUP-AT, and MiaPaCa-2 cells was higher in hypoxia than that in normoxia.
Cells (2 x 106 cells/mL) were fixed with 2% paraformaldehyde and incubated in PBS with anti IGF1R antibody (Anti-IGF1 Receptor antibody [alphaIR3] ab16890, Abcam) or mouse IgG1- isotype control (ab91353, Abcam) for 30 minutes at 22°C. Cells were subsequently labeled with FITC-conjugated secondary antibody (1:500; Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879, Abcam) for 30 minutes at 22°C.
The percentage of positive cells were calculated and compared with isotype-matched control-stained cells.
(A) Negative control (×200).
(C) SHADR group: Diffuse glomerular nestin expression was detected involving almost all podocytes within glomerulus. After losartan and tempol treatment, either single or in combination, kidneys restored nestin expression similar to controls (SHC group).
Immunostaining was applied on 5 μm thick paraffin sections. After deparaffinization and rehydration, the sections were treated by microwave for 20 minutes at 400 W in citrate buffer (pH 6.0). After antigen retrieval, samples were incubated for 1 hour at room temperature with primary antibody for nestin (dilution 1:100). Sections were then treated using 3-amino-9-ethylcarbazole (AEC) as substrate, and counterstained with hematoxylin. Negative controls were performed by omitting the first antibody and mouse monoclonal antibodies as isotype control mouse IgG1 (ab91353) antibody was also used.
Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with Anti-CD82 antibody [TS82b] - BSA and Azide free ab59509 (red line).
The cells were fixed with 80% methanol (5 minutes) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-CD82 antibody [TS82b] - BSA and Azide free ab59509, 1 μg/1x106 cells) for 30 minutes at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 minutes at 22°C.
Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 μg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed.
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