Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control

12 product images

• 

  Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

  Histone H3 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551 at 1/50 dilution (2ug in 0.4mg lysates). Western blot was performed on the immunoprecipitate using Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551 at 1/1000 dilution. mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/5000 dilution.
  Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
  Lane 2: Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
  Lane 3: Mouse IgG1 monoclonal isotype control (ab18443) instead of Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551 in NIH/3T3 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 32 seconds

  All lanes: Immunoprecipitation - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker (Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551) at 1/50 dilution

  All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) at 1/5000 dilution

  Exposure time: 32s

• 

  Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

  Histone H3 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551 at 1/50 dilution (2ug in 0.4mg lysates). Western blot was performed on the immunoprecipitate using Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551 at 1/1000 dilution. mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/5000 dilution.
  Lane 1: C6 (rat glial tumor glial cell) whole cell lysate
  Lane 2: Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551 IP in C6 (rat glial tumor glial cell) whole cell lysate
  Lane 3: Mouse IgG1 monoclonal isotype control (ab18443) instead of Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551 in C6 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 67 seconds

  All lanes: Immunoprecipitation - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker (Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551) at 1/50 dilution

  All lanes: C6 (rat glial tumor glial cell) whole cell lysate at 10 µg

  Secondary

  All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) at 1/5000 dilution

  Exposure time: 67s

• 

  Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

  Histone H3 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551 at 1/50 dilution (2ug in 0.4mg lysates). Western blot was performed on the immunoprecipitate using Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551 at 1/1000 dilution. mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/5000 dilution.
  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
  Lane 2: Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
  Lane 3: Mouse IgG1 monoclonal isotype control (ab18443) instead of Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551 in HeLa whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 32 seconds

  All lanes: Immunoprecipitation - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker (Anti-Histone H3 antibody [1B1B2] - Nuclear Marker ab309551) at 1/50 dilution

  All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) at 1/5000 dilution

  Exposure time: 32s

• 

  Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

  Histone H2B was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade ab52484 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade ab52484 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. 
  
  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate  10 ug
  
  Lane 2: Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade ab52484 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
  
  Lane 3: Mouse monoclonal IgG (ab18443) instead of Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade ab52484 in HeLa whole cell lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 32 seconds.

  All lanes: Immunoprecipitation - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade ab52484) at 1/30 dilution

  All lanes: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Predicted band size: 14 kDa

  Observed band size: 15 kDa

• 

  Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

  Histone H2B was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate with Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade ab52484 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade ab52484 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. 
  
  Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 μg
  
  Lane 2: Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade ab52484 IP in NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 μg
  
  Lane 3: Mouse monoclonal IgG (ab18443) instead of Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade ab52484 in NIH/3T3 whole cell lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 32 seconds.

  All lanes: Immunoprecipitation - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade ab52484) at 1/30 dilution

  All lanes: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

• 

  Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

  SH3GL1/Endophilin A2 was immunoprecipitated from 0.35 mg Rat brain tissue lysate with Anti-SH3GL1/Endophilin A2 antibody [K51/1] ab317835 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SH3GL1/Endophilin A2 antibody [K51/1] ab317835 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

  Lane 1: Rat brain tissue lysate
  
  Lane 2: Anti-SH3GL1/Endophilin A2 antibody [K51/1] ab317835 IP in Rat brain tissue lysate
  
  Lane 3: Mouse monoclonal IgG (ab18443) instead of Anti-SH3GL1/Endophilin A2 antibody [K51/1] ab317835 in rat testis tissue lysate

  All lanes: Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (Anti-SH3GL1/Endophilin A2 antibody [K51/1] ab317835) at 1/30 dilution

  All lanes: Rat brain tissue lysate at 0.35 mg with NFDM/TBST

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 41s

• 

  Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

  SH3GL1/Endophilin A2 was immunoprecipitated from 0.35 mg Rat testis tissue lysate with Anti-SH3GL1/Endophilin A2 antibody [K51/1] ab317835 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SH3GL1/Endophilin A2 antibody [K51/1] ab317835 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

  Lane 1: Rat testis tissue lysate
  
  Lane 2: Anti-SH3GL1/Endophilin A2 antibody [K51/1] ab317835 IP in Rat testis tissue lysate
  
  Lane 3: Mouse monoclonal IgG (ab18443) instead of Anti-SH3GL1/Endophilin A2 antibody [K51/1] ab317835 in rat testis tissue lysate

  All lanes: Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (Anti-SH3GL1/Endophilin A2 antibody [K51/1] ab317835) at 1/30 dilution

  All lanes: Rat testis tissue lysate with NFDM/TBST

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 41s

• 

  Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

  CP2c was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate 10 ug with Anti-CP2c antibody [14/LSF] ab308611 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CP2c antibody [14/LSF] ab308611 at 1/1000 dilution. Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) was used at 1/10000 dilution.
  Lane 1: C6 (rat glial tumor glial cell) whole cell lysate
  Lane 2: Anti-CP2c antibody [14/LSF] ab308611 IP in C6 (rat glial tumor glial cell) whole cell lysate
  Lane 3: Mouse monoclonal IgG (ab18443) instead of Anti-CP2c antibody [14/LSF] ab308611 in C6 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 59 seconds

  All lanes: Immunoprecipitation - Anti-CP2c antibody [14/LSF] (Anti-CP2c antibody [14/LSF] ab308611) at 1/30 dilution

  All lanes: C6 (rat glial tumor glial cell) whole cell lysate

  Secondary

  All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

  Exposure time: 59s

• 

  Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

  CP2c was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate 10 ug with Anti-CP2c antibody [14/LSF] ab308611 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CP2c antibody [14/LSF] ab308611 at 1/1000 dilution. Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) was used at 1/10000 dilution.
  Lane 1: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate
  Lane 2: Anti-CP2c antibody [14/LSF] ab308611 IP in Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate
  Lane 3: Mouse monoclonal IgG (ab18443) instead of Anti-CP2c antibody [14/LSF] ab308611 in Hepa1-6 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 59 seconds

  All lanes: Immunoprecipitation - Anti-CP2c antibody [14/LSF] (Anti-CP2c antibody [14/LSF] ab308611) at 1/30 dilution

  All lanes: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate

  Secondary

  All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

  Exposure time: 59s

• 

  Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

  CP2c was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug with Anti-CP2c antibody [14/LSF] ab308611 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CP2c antibody [14/LSF] ab308611 at 1/1000 dilution. mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/1000 dilution.
  Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
  Lane 2: Anti-CP2c antibody [14/LSF] ab308611 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
  Lane 3: Mouse monoclonal IgG (ab18443) instead of Anti-CP2c antibody [14/LSF] ab308611 in K-562 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 24 seconds

  All lanes: Immunoprecipitation - Anti-CP2c antibody [14/LSF] (Anti-CP2c antibody [14/LSF] ab308611) at 1/30 dilution

  All lanes: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) at 1/1000 dilution

  Exposure time: 24s

• 

  Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

  SHP2 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 µg with Anti-SHP2 antibody [79/PTP1D/SHP2] ab290646 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SHP2 antibody [79/PTP1D/SHP2] ab290646 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  
  Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 µg
  
  Lane 2: Anti-SHP2 antibody [79/PTP1D/SHP2] ab290646 IP in NIH/3T3 whole cell lysate
  
  Lane 3: Mouse IgG1 monoclonal (ab18443) instead of Anti-SHP2 antibody [79/PTP1D/SHP2] ab290646 in NIH/3T3 whole cell lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 3 seconds

  Lane 1: Immunoprecipitation - Anti-SHP2 antibody [79/PTP1D/SHP2] (Anti-SHP2 antibody [79/PTP1D/SHP2] ab290646) at 1/30 dilution

  Lane 2: Immunoprecipitation - Anti-SHP2 antibody [79/PTP1D/SHP2] (Anti-SHP2 antibody [79/PTP1D/SHP2] ab290646) at 1/1000 dilution

  All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

  Secondary

  All lanes: Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443) at 1/5000 dilution

  Predicted band size: 68 kDa

  Exposure time: 3s

• 

  Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

  p53 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with Anti-p53 antibody [G59-12] ab308609 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-p53 antibody [G59-12] ab308609 at 1/1000 dilution. mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/5000 dilution.
  
  Lane 1: HEK-293 whole cell lysate
  
  Lane 2: Anti-p53 antibody [G59-12] ab308609 IP in HEK-293 whole cell lysate
  
  Lane 3: Mouse IgG1 monoclonal isotype control (ab18443) instead of Anti-p53 antibody [G59-12] ab308609 in HEK-293 whole cell lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 23 seconds.
  
  The binding pattern observed is consistent with what has been described in the literature (PMID: 31045216; PMID: 16131611).

  All lanes: Immunoprecipitation - Anti-p53 antibody [G59-12] (Anti-p53 antibody [G59-12] ab308609) at 1/1000 dilution

  All lanes: HEK-293 whole cell lysate at 10 µg

  Secondary

  All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) at 1/5000 dilution