AB190463

Anti-Mouse IgG2a antibody [RM107]

Recombinant
What is this?

Be the first to review this product! Submit a review

(4 Publications )

Rabbit Recombinant Monoclonal GCAA antibody. Suitable for IP, Flow Cyt, ELISA, WB, IHC-P, ICC/IF and reacts with Recombinant fragment, Mouse, Recombinant full length protein - Mouse samples. Cited in 4 publications. Immunogen corresponding to Full Length Protein corresponding to Mouse Ighg.

3 Images

ELISA - Anti-Mouse IgG2a antibody [RM107] (AB190463)

ELISA

Supplier Data

ELISA - Anti-Mouse IgG2a antibody [RM107] (AB190463)

ELISA analysis of mouse IgG2a using ab190463 at different concentrations. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA

Supplier Data

ELISA - Anti-Mouse IgG2a antibody [RM107] (AB190463)

ELISA analysis of mouse immunoglobulins labeling the Fab region of mouse IgG2a using ab190463 at 200 ng/ml, 50 ng/ml, or 10 ng/ml. The plate was coated with 50 ng/well of different immunoglobulins. No cross reactivity with IgG2aλ, IgG1, IgG3, IgM, IgA, IgE, Human IgG, rat IgG, or goat IgG was detected. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Supplier Data

Western blot - Anti-Mouse IgG2a antibody [RM107] (AB190463)

All lanes:

Western blot - Anti-Mouse IgG2a antibody [RM107] (ab190463) at 0.2 µg/mL

Lane 1:

Mouse IgG2a kappa (non-reducing conditions) at 0.02 µg

Lane 2:

Mouse IgG2a kappa (reducing conditions) at 0.02 µg

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM107

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

Flow Cyt, ICC/IF, IHC-P, WB, IP, ELISA

applications

Immunogen

Full Length Protein corresponding to Mouse Ighg. The exact immunogen used to generate this antibody is proprietary information.

P01863

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "Non-reducing conditions only", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.005-0.2 µg/mL", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Protein A affinity purified from an animal origin-free and protein-free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Ighg

Alternative Names

Immunoglobulin heavy chain gamma polypeptide, Ighg

style

left-column

Publications (4)

Recent publications for all applications. Explore the full listand refine your search

Life science alliance 8: PubMed40957650

2025

CTPS2 regulates CTP synthetase activity by interacting with CTPS1.

Applications

Unspecified application

Species

Unspecified reactive species

Norbert Minet,Benoît Heid-Picard,Vanessa Masson,Damarys Loew,Emmanuel Martin,Sylvain Latour

Cancers 15: PubMed37894441

2023

New Insights on the Progesterone (P4) and PGRMC1/NENF Complex Interactions in Colorectal Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Kamińska,Olga Martyna Koper-Lenkiewicz,Donata Ponikwicka-Tyszko,Weronika Lebiedzińska,Ewelina Palak,Maria Sztachelska,Piotr Bernaczyk,Justyna Dorf,Katarzyna Guzińska-Ustymowicz,Konrad Zaręba,Sławomir Wołczyński,Nafis Ahmed Rahman,Violetta Dymicka-Piekarska

International journal of oncology 63: PubMed37654195

2023

Human cytomegalovirus infection enhances 5‑lipoxygenase and cycloxygenase‑2 expression in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mattia Russel Pantalone,Nerea Martin Almazan,Rossano Lattanzio,Chato Taher,Simone De Fabritiis,Silvia Valentinuzzi,Faraz Bishehsari,Mahboobeh Mahdavinia,Fabio Verginelli,Afsar Rahbar,Renato Mariani-Costantini,Cecilia Söderberg-Naucler

Cancers 12: PubMed33158280

2020

Mifepristone Treatment Promotes Testicular Leydig Cell Tumor Progression in Transgenic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Donata Ponikwicka-Tyszko,Marcin Chrusciel,Kamila Pulawska,Piotr Bernaczyk,Maria Sztachelska,Peilan Guo,Xiangdong Li,Jorma Toppari,Ilpo T Huhtaniemi,Slawomir Wołczyński,Nafis A Rahman

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mouse IgG2a antibody [RM107]

ELISA - Anti-Mouse IgG2a antibody [RM107] (AB190463)

ELISA - Anti-Mouse IgG2a antibody [RM107] (AB190463)

Western blot - Anti-Mouse IgG2a antibody [RM107] (AB190463)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Alternative Names

Publications (4)

Product promise