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AB91361

Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control

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(16 Publications)

Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control Suitable for Flow Cyt and reacts with samples. Cited in 16 publications.
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Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (AB91361)
  • Flow Cyt

Supplier Data

Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (AB91361)

Flow cytometry analysis of normal whole blood sample labeling IgG2a with ab91361 at 1μg.

Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (AB91361)
  • Flow Cyt

Unknown

Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (AB91361)

Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • 660 APC

    APC Mouse IgG2a [B12/8] - Isotype Control

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B12/8

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p>ab91361 is a negative isotype control</p>" } } }
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Product details

Immunogen
Synthetic peptide. Hapten from sequence of plant origin.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from supernatant from an in vitro culture.
Storage buffer
pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 19:2789-2799 PubMed37335017

2023

Measurement of SQSTM1 by flow cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica C Hargarten,Guowu Hu,Waleed Elsegeiny,Peter R Williamson

Autophagy 19:678-691 PubMed35838483

2022

Autophagy-associated immune dysregulation and hyperplasia in a patient with compound heterozygous mutations in .

Applications

Unspecified application

Species

Unspecified reactive species

Guowu Hu,Pia J Hauk,Nannan Zhang,Waleed Elsegeiny,Carlos M Guardia,Amy Kullas,Kevin Crosby,Robin R Deterding,Michaela Schedel,Paul Reynolds,Jordan K Abbott,Vijaya Knight,Stefania Pittaluga,Mark Raffeld,Sergio D Rosenzweig,Juan S Bonifacino,Gulbu Uzel,Peter R Williamson,Erwin W Gelfand

Journal of viral hepatitis 29:124-134 PubMed34820942

2021

Intrahepatic immune infiltrate in chronic hepatitis B and chronic hepatitis C: Similar but not the same.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilia Graciela Giadans,Daniela Alejandra Ríos,Beatriz Ameigeiras,Leila Haddad,Elena Noemí De Matteo,Pamela Valva,María Victoria Preciado

Nutrients 13: PubMed34836359

2021

Creatine and Nicotinamide Prevent Oxidant-Induced Senescence in Human Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Avinash S Mahajan,Venkata S Arikatla,Anita Thyagarajan,Tetyana Zhelay,Ravi P Sahu,Michael G Kemp,Dan F Spandau,Jeffrey B Travers

Annals of neurology 88:1205-1219 PubMed32951274

2020

Keratinocytes Communicate with Sensory Neurons via Synaptic-like Contacts.

Applications

Unspecified application

Species

Unspecified reactive species

Matthieu Talagas,Nicolas Lebonvallet,Raphael Leschiera,Gerard Sinquin,Philippe Elies,Marek Haftek,Jean-Pierre Pennec,Denis Ressnikoff,Veronica La Padula,Raphaele Le Garrec,Killian L'herondelle,Olivier Mignen,Laetitia Le Pottier,Nathalie Kerfant,Alexia Reux,Pascale Marcorelles,Laurent Misery

Experimental dermatology 29:387-392 PubMed32003039

2020

Intra-epidermal nerve endings progress within keratinocyte cytoplasmic tunnels in normal human skin.

Applications

Unspecified application

Species

Unspecified reactive species

Matthieu Talagas,Nicolas Lebonvallet,Raphael Leschiera,Philippe Elies,Pascale Marcorelles,Laurent Misery

Acta physiologica (Oxford, England) 228:e13325 PubMed31162891

2019

AKAP12 deficiency impairs VEGF-induced endothelial cell migration and sprouting.

Applications

Unspecified application

Species

Unspecified reactive species

Peter M Benz,Yindi Ding,Heike Stingl,Annemarieke E Loot,Joana Zink,Ilka Wittig,Rüdiger Popp,Ingrid Fleming

Free radical biology & medicine 139:80-91 PubMed31078730

2019

Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8 T cells activation via JAK-STAT pathway in vitiligo.

Applications

Unspecified application

Species

Unspecified reactive species

Xuguang Chen,Weinan Guo,Yuqian Chang,Jiaxi Chen,Pan Kang,Xiuli Yi,Tingting Cui,Sen Guo,Qian Xiao,Zhe Jian,Kai Li,Tianwen Gao,Shuli Li,Ling Liu,Chunying Li

Journal of viral hepatitis 26:727-737 PubMed30739377

2019

Chronic hepatitis B: The interplay between intrahepatic lymphocyte population and viral antigens in relation to liver damage.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilia G Giadans,Daniela A Ríos,Beatriz Ameigeiras,Adriana M Pietrantonio,Néstor L Lucatelli,Leila Haddad,Eduardo Mullen,Fabiana Heinrich,Elena De Matteo,Diego Flichman,Pamela Valva,María V Preciado

Molecular neurobiology 56:1262-1275 PubMed29881947

2018

Tropomyosin-Related Kinase B (TrkB) Regulates Neurite Outgrowth via a Novel Interaction with Suppressor of Cytokine Signalling 2 (SOCS2).

Applications

Unspecified application

Species

Unspecified reactive species

Akram Zamani,Junhua Xiao,Ann M Turnley,Simon S Murray
View all publications
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