Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control Suitable for Flow Cyt and reacts with samples. Cited in 9 publications.
Images
![Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (AB91361), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg2a-kappa-monoclonal-b12-8-isotype-control-ab91361--flow-cytometry-img103416.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (AB91361)")
![Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (AB91361), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg2a-kappa-monoclonal-b12-8-isotype-control-ab91361--flow-cytometry-img80622.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (AB91361)")
Publications
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- Journal of viral hepatitis 29:124-1342021Intrahepatic immune infiltrate in chronic hepatitis B and chronic hepatitis C: Similar but not the same.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCecilia Graciela Giadans,Daniela Alejandra Ríos,Beatriz Ameigeiras,Leila Haddad,Elena Noemí De Matteo,Pamela Valva,María Victoria PreciadoPubMed 34820942
- Nutrients 13:2021Creatine and Nicotinamide Prevent Oxidant-Induced Senescence in Human Fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAvinash S Mahajan,Venkata S Arikatla,Anita Thyagarajan,Tetyana Zhelay,Ravi P Sahu,Michael G Kemp,Dan F Spandau,Jeffrey B TraversPubMed 34836359
- Acta physiologica (Oxford, England) 228:e133252019AKAP12 deficiency impairs VEGF-induced endothelial cell migration and sprouting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeter M Benz,Yindi Ding,Heike Stingl,Annemarieke E Loot,Joana Zink,Ilka Wittig,Rüdiger Popp,Ingrid FlemingPubMed 31162891
- Free radical biology & medicine 139:80-912019Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8 T cells activation via JAK-STAT pathway in vitiligo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuguang Chen et. al.PubMed 31078730
- Scientific reports 7:150102017Mesenchymal stem cells internalize Mycobacterium tuberculosis through scavenger receptors and restrict bacterial growth through autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArshad Khan et. al.PubMed 29118429
- Scientific reports 7:132832017Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniela Alejandra Rios et. al.PubMed 29038590
- Journal of inflammation (London, England) 12:282015Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients.
- PloS one 10:e01183102015Design of a multicomponent peptide-woven nanocomplex for delivery of siRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEunsung Jun,Soyoun Kim,Jong-Ho Kim,Kiweon Cha,In-Seop So,Hye-Nam Son,Byung-Heon Lee,Kwangmeyung Kim,Ick Chan Kwon,Sang Yoon Kim,In-San KimPubMed 25705892
- The Journal of biological chemistry 289:11132-422014Antibodies that detect O-linked β-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins.Applications:Flow CytReactive species:Unspecified reactive speciesYuko Tashima et. al.PubMed 24573683
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info 1 µg for 106 Cells | Notes ab91361 is a negative isotype control |
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Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control Suitable for Flow Cyt and reacts with samples. Cited in 9 publications.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- B12/8
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from supernatant from an in vitro culture.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Immunogen
Synthetic peptide. Hapten from sequence of plant origin.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
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2 product images
![Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg2a-kappa-monoclonal-b12-8-isotype-control-ab91361--flow-cytometry-img103416.jpg "Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)")
Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
Flow cytometry analysis of normal whole blood sample labeling IgG2a with ab91361 at 1μg.
![Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg2a-kappa-monoclonal-b12-8-isotype-control-ab91361--flow-cytometry-img80622.jpg "Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)")
Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
Overlay histogram showing A549 cells stained with Anti-MUC4 antibody [5B12] ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-MUC4 antibody [5B12] ab60720, 0.5μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
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