Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade

• IP

Lab

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

Viperin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab107359 at 1/50 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab107359 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] (<a href='/en-us/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a>) at 1/50 dilution

Lane 1:

Mouse spleen tissue lysate (Input) at 10 µg

Lane 2:

Mouse spleen tissue lysate (+)

Lane 3:

Mouse IgG2a, kappaIsotype control (ab18413) instead of <a href='/en-us/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a> in KG-1a whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 93s

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

Flow cytometry overlay histogram showing wild-type p53 in Hek-293 positive cells (left) and MCF7 negative cells (right) stained with ab1101 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab1101) (1x 106 cells in 100μl at 0.2μg/ml (1/5000)) for 30min at 22°C. The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) was incubated at 1/4000 for 30min at 22°C. Isotype control antibody (black line) Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. This antibody gave a positive signal in Hek-293 fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

Flow cytometry overlay histogram showing mutant p53 in A-431 cells stained with ab1101 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab1101) (1x 106 cells in 100μl at 0.04μg/ml (1/25000)) for 30min at 22°C. The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) was incubated at 1/4000 for 30min at 22°C. Isotype control antibody (black line) Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. This antibody gave a positive signal in A-431 fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

Flow cytometry overlay histogram showing mutant p53 in wild-type HAP1 (green line) and TP53 knockout HAP1 cells (red line) stained with ab1101. The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab1101) (1x 106 cells in 100μl at 0.04 μg/ml (1/25000)) for 30min at 22°C.The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) was incubated at 1/4000 for 30min at 22°C. Isotype control antibody Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413) was used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line, TP53 knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity). Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. This antibody gave a positive signal in HAP1 fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

• Flow Cyt

Supplier Data

Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

• Flow Cyt

Lab

Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

Flow cytometry overlay histogram showing left Jurkat positive cells and right negative HeLa stained with ab230296 (red line). The cells were incubated in 1x PBS containing 10% human IgG and 10%; normal goat serum to block non-specific protein-protein interaction followed by the antibody ab230296 at 1 μg/ml for 30min on ice.

The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor^® 488, pre-adsorbed) (ab150177) was used at 1/4000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a (ab18413) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable single cells.

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

gamma Catenin was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab305241 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305241 at dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-gamma Catenin antibody [15/γ-Catenin] (<a href='/en-us/products/primary-antibodies/gamma-catenin-antibody-15-catenin-ab305241'>ab305241</a>) at 1/1000 dilution

Lane 1:

MCF7 whole cell lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/gamma-catenin-antibody-15-catenin-ab305241'>ab305241</a> at 1/30 IP in MCF7 whole cell lysate at 10 µg

Lane 3:

Mouse IgG2a monoclonal isotype control (ab18413) instead of <a href='/en-us/products/primary-antibodies/gamma-catenin-antibody-15-catenin-ab305241'>ab305241</a> in MCF7 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 82 kDa

false

Exposure time: 67s

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

All lanes:

Immunoprecipitation - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/rab8a-antibody-mjf-r22-79-3-mouse-igg2a-chimeric-ab307607'>ab307607</a>) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole cell lysate 10 μg

Lane 2:

A549 whole cell lysate

Observed band size: 23 kDa

false

Exposure time: 24s

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

All lanes:

Immunoprecipitation - Anti-RILPL1 antibody [MJF-R41-21] - Mouse IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/rilpl1-antibody-mjf-r41-21-mouse-igg2a-chimeric-ab307294'>ab307294</a>) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/rilpl1-antibody-mjf-r41-21-mouse-igg2a-chimeric-ab307294'>ab307294</a> at 1/30 IP in A549 (human lung carcinoma epithelial cell) at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 47 kDa

true

Exposure time: 3min

• IP

Lab

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

Viperin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab107359 at 1/50 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab107359 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] (<a href='/en-us/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a>) at 1/50 dilution

Lane 1:

KG-1a (human bone marrow myeloblast) whole cell lysate (Input) at 10 µg

Lane 2:

KG-1a (human bone marrow myeloblast) whole cell lysate (+)

Lane 3:

Mouse IgG2a, kappaIsotype control (ab18413) instead of <a href='/en-us/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a> in KG-1a whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 93s

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

Crk p38 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab300630 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300630 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

ab300630 recognises a band at ~35 KDa (isoform Crk-II) and ~23KDa (isoform Crk-I).

All lanes:

Immunoprecipitation - Anti-Crk p38 antibody [22/Crk] (<a href='/en-us/products/primary-antibodies/crk-p38-antibody-22-crk-ab300630'>ab300630</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/crk-p38-antibody-22-crk-ab300630'>ab300630</a> at 1/30 IP in HeLa whole cell lysate at 10 µg

Lane 3:

Mouse IgG2a, kappa monoclonal isotype control (ab18413) instead of <a href='/en-us/products/primary-antibodies/crk-p38-antibody-22-crk-ab300630'>ab300630</a> in HeLa whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 33 kDa

Observed band size: 23 kDa,35 kDa

true

Exposure time: 3min

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

RAB10 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate 10 ug with ab307296 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307296 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate Lane 2 : ab307296 IP in A549 (human lung carcinoma epithelial cell) whole cell lysate Lane 3 : Mouse IgG2a Isotype control (ab18413) instead of ab307296 in A549 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/rab10-antibody-mjf-r23-mouse-igg2a-chimeric-ab307296'>ab307296</a>) at 1/30 dilution

All lanes:

A549 (human lung carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

false

Exposure time: 15s

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

Overlay histogram showing SV40LT-SMC cells stained with ab7817 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab7817, 5μg/ml) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L - preadsorbed (Alexa Fluor^® 488) (ab150117) at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [MOPC-173] (ab18413) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

• Flow Cyt

Supplier Data

Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab320707 (right) or Mouse IgG2a, kappa monoclonal (ab18413), isotype control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10%rat serum 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab320707 or Mouse IgG2a, kappa monoclonal (ab18413), isotype control (1x 10e6 in 100 μl at 1.0 μg/ml ) for 30min on ice. The cells were simultaneously stained with APC anti-mouse Ly-6C Antibody.

The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

Crk p38 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab300630 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300630 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

ab300630 recognises a band at ~35 KDa (isoform Crk-II) and ~23KDa (isoform Crk-I).

All lanes:

Immunoprecipitation - Anti-Crk p38 antibody [22/Crk] (<a href='/en-us/products/primary-antibodies/crk-p38-antibody-22-crk-ab300630'>ab300630</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/crk-p38-antibody-22-crk-ab300630'>ab300630</a> at 1/30 IP in NIH/3T3 whole cell lysate at 10 µg

Lane 3:

Mouse IgG2a, kappa monoclonal isotype control (ab18413) instead of <a href='/en-us/products/primary-antibodies/crk-p38-antibody-22-crk-ab300630'>ab300630</a> in NIH/3T3 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 33 kDa

Observed band size: 23 kDa,35 kDa

true

Exposure time: 3min

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

Crk p38 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with ab300630 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300630 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

ab300630 recognises a band at ~35 KDa (isoform Crk-II) and ~23KDa (isoform Crk-I).

All lanes:

Immunoprecipitation - Anti-Crk p38 antibody [22/Crk] (<a href='/en-us/products/primary-antibodies/crk-p38-antibody-22-crk-ab300630'>ab300630</a>) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/crk-p38-antibody-22-crk-ab300630'>ab300630</a> at 1/30 IP in C6 whole cell lysate at 10 µg

Lane 3:

Mouse IgG2a, kappa monoclonal isotype control (ab18413) instead of <a href='/en-us/products/primary-antibodies/crk-p38-antibody-22-crk-ab300630'>ab300630</a> in C6 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 33 kDa

Observed band size: 23 kDa,35 kDa

true

Exposure time: 3min

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

RAB10 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with ab307296 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307296 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 2 : ab307296 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 3 : Mouse IgG2a Isotype control (ab18413) instead of ab307296 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/rab10-antibody-mjf-r23-mouse-igg2a-chimeric-ab307296'>ab307296</a>) at 1/30 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

false

Exposure time: 15s

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

All lanes:

Immunoprecipitation - Anti-RILPL1 antibody [MJF-R41-21] - Mouse IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/rilpl1-antibody-mjf-r41-21-mouse-igg2a-chimeric-ab307294'>ab307294</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/rilpl1-antibody-mjf-r41-21-mouse-igg2a-chimeric-ab307294'>ab307294</a> at 1/30 IP in rat brain tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 47 kDa

true

Exposure time: 3min

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

RAB10 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate 10 ug with ab307296 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307296 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate Lane 2 : ab307296 IP in C6 (rat glial tumor glial cell) whole cell lysate Lane 3 : Mouse IgG2a Isotype control (ab18413) instead of ab307296 in C6 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/rab10-antibody-mjf-r23-mouse-igg2a-chimeric-ab307296'>ab307296</a>) at 1/30 dilution

All lanes:

C6 (rat glial tumor glial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

false

Exposure time: 15s

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

All lanes:

Immunoprecipitation - Anti-RILPL1 antibody [MJF-R41-21] - Mouse IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/rilpl1-antibody-mjf-r41-21-mouse-igg2a-chimeric-ab307294'>ab307294</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lanes 1 - 2:

<a href='/en-us/products/primary-antibodies/rilpl1-antibody-mjf-r41-21-mouse-igg2a-chimeric-ab307294'>ab307294</a> at 1/30 IP in mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 47 kDa

true

Exposure time: 3min

• ChIP

PubMed

ChIP - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

ChIP of ΔNp73 binding with SBE. The relative amount of ΔNp73 associated DNA as pulled down with an antibody directed against HA, is represented as a fold enrichment compared to pull-down with IgG (background, ab18413). Gene enrichment was quantified by qPCR using primers specific for the promoter regions of A) PAI-1, B) Col1a1 and C) p21^WAF within the SBEs. Primers specific for PTEN (D) were used as a positive control. Pulldown antibody is shown on the x-axis, with y-axis showing fold enrichment ± SEM. * represents p-value>0.05.

Image from Niemantsverdriet M et al., PLoS One. 2012;7(12):e50815. Fig 5.; doi: 10.1371/journal.pone.0050815. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

HIV1 integrase was immunoprecipitated from 0.35 mg 293T cells transfected with a human immunodeficiency virus type 1 group M subtype B (isolate NY5) (HIV-1) integrase expression vector containing a myc-his-tag whole cell lysate with ab323451 at 1/50 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323451 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Mouse HIV1 integrase antibody [IN-4] (<a href='/en-us/products/primary-antibodies/mouse-hiv1-integrase-antibody-in-4-ab323451'>ab323451</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a human immunodeficiency virus type 1 group M subtype B (isolate NY5) (HIV-1) integrase expression vector containing a myc-his-tag whole cell lysate at 4 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/mouse-hiv1-integrase-antibody-in-4-ab323451'>ab323451</a> at 1/30 IP in 293T cells transfected with a human immunodeficiency virus type 1 group M subtype B (isolate NY5) (HIV-1) integrase expression vector containing a myc-his-tag whole cell lysate at 4 µg

Lane 3:

Mouse IgG2a, Kappa monoclonal [MOPC-173] -isotype control (ab18413) instead of <a href='/en-us/products/primary-antibodies/mouse-hiv1-integrase-antibody-in-4-ab323451'>ab323451</a> in 293T cells transfected with a human immunodeficiency virus type 1 group M subtype B (isolate NY5) (HIV-1) integrase expression vector containing a myc-his-tag whole cell lysate at 4 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 37 kDa

false

Exposure time: 3s

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

HIV1 integrase was immunoprecipitated from 0.35 mg 293T cells transfected with a human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1) integrase expression vector containing a myc-his-tag whole cell lysate with ab323451 at 1/50 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323451 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Mouse HIV1 integrase antibody [IN-4] (<a href='/en-us/products/primary-antibodies/mouse-hiv1-integrase-antibody-in-4-ab323451'>ab323451</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1) integrase expression vector containing a myc-his-tag whole cell lysate at 4 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/mouse-hiv1-integrase-antibody-in-4-ab323451'>ab323451</a> at 1/30 IP in 293T cells transfected with a human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1) integrase expression vector containing a myc-his-tag whole cell lysate at 4 µg

Lane 3:

Mouse IgG2a, Kappa monoclonal [MOPC-173] -isotype control (ab18413) instead of <a href='/en-us/products/primary-antibodies/mouse-hiv1-integrase-antibody-in-4-ab323451'>ab323451</a> in 293T cells transfected with a human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1) integrase expression vector containing a myc-his-tag whole cell lysate at 4 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 37 kDa

false

Exposure time: 3s

• IP

Supplier Data

Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (AB18413)

HSV1 gH was immunoprecipitated from 0.35 mg of 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His- tag whole cell lysate with ab110227 at 1/50 dilution (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab110227 at 1/1000 dilution. ab131366, VeriBlot for IP secondary antibody(HRP) was used as secondary antibody at 1/5000 dilution.

Lane 1(Input) : 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag whole cell lysate, 10 μg

Lane 2(+) : 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag whole cell lysate

Lane 3(-) : Mouse IgG2a kppa monoclonal isotype control (ab18413) instead of ab110227 in 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag whole cell lysate

Observed MW(KDa) : 100

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-HSV1 gH antibody [BBH1] (<a href='/en-us/products/primary-antibodies/hsv1-gh-antibody-bbh1-ab110227'>ab110227</a>) at 1/50 dilution

All lanes:

293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 100 kDa

false

Exposure time: 180s