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AB190482

Anti-Mouse IgG2b antibody [RM108]

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Rabbit Recombinant Monoclonal IGG2B antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Full Length Protein corresponding to Mouse Ighg2b.

View Alternative Names

Igh-3, Ighg2b, Immunoglobulin heavy constant gamma 2B, Ig gamma-2B chain C region

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse IgG2b antibody [RM108] (AB190482)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse IgG2b antibody [RM108] (AB190482)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling HLA-DOB with ab312858 at 1/20000 (0.053 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human skeletal muscle. The section was incubated with ab312858 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse IgG2b antibody [RM108] (AB190482)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse IgG2b antibody [RM108] (AB190482)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling HLA-DOB with ab312858 at 1/20000 (0.053 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil. The section was incubated with ab312858 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Western blot - Anti-Mouse IgG2b antibody [RM108] (AB190482)
  • WB

Supplier Data

Western blot - Anti-Mouse IgG2b antibody [RM108] (AB190482)

ab190482 reacted to non-reduced IgG2b (~150 kDa) much stronger than the reduced γ2b(~50 kDa).

All lanes:

Western blot - Anti-Mouse IgG2b antibody [RM108] (ab190482) at 0.2 µg/mL

Lane 1:

Mouse IgG2b, non-reduced at 0.02 µg

Lane 2:

Mouse IgG2b, reduced at 0.02 µg

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM108

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Full Length Protein corresponding to Mouse Ighg2b. The exact immunogen used to generate this antibody is proprietary information.

P01867

Specificity

ab190482 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The Fc region of ab190482 has been engineered to eliminate the binding of Fc receptor.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab190482 is purified from an animal origin–free and protein-free culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Ighg2b
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