AB221850

Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free

Name: Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free (ab221850) | Abcam
Brand: Abcam Limited
SKU: AB221850
Price: 905 USD
Availability: InStock

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(1 Publication)

Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free Suitable for ICC/IF, Flow Cyt, ELISA and reacts with samples. Cited in 1 publication.

1 Images

Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free (AB221850)

Flow Cyt

Unknown

Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free (AB221850)

This flow cytometry data was generated using the same antibody clone under a different buffer format (cat# ab170192).

Flow cytometry experiments with 4% PFA fixed Hela (human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast)were performed with the IgG2b Isotype Control Antibody (red) and no primary antibody negative control (black). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody.

The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

617 Alexa Fluor® 594

Alexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control
Unconjugated

Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7E10G10

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Applications

ELISA, Flow Cyt, ICC/IF

applications

Specificity

This Mouse IgG2b Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Product details

This antibody clone is manufactured by Abcam.

ab221850 is a PBS only buffer formulated version of ab170192.

Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody's host species, isotype, and possible conjugation.

If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Storage buffer

pH: 7.5 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of 117:25712-25721 PubMed32989127

2020

Genetic landscape and autoimmunity of monocytes in developing Vogt-Koyanagi-Harada disease.

Applications

Unspecified application

Species

Unspecified reactive species

Youjin Hu,Yixin Hu,Yuhua Xiao,Feng Wen,Shaochong Zhang,Dan Liang,Lishi Su,Yang Deng,Jiawen Luo,Jingsong Ou,Mingzhi Lu,Yanhua Hong,Wei Chi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free

Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free (AB221850)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (1)

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