Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free Suitable for ICC/IF, Flow Cyt, ELISA and reacts with samples. Cited in 1 publication.
Images
![Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free (AB221850), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-bsa-and-azide-free-ab221850--flow-cytometry-img71328.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free (AB221850)")
Publications
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- Proceedings of the National Academy of Sciences of 117:25712-257212020Genetic landscape and autoimmunity of monocytes in developing Vogt-Koyanagi-Harada disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoujin Hu et. al.PubMed 32989127
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.5
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC/IF | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free Suitable for ICC/IF, Flow Cyt, ELISA and reacts with samples. Cited in 1 publication.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.5
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- 7E10G10
- Purification technique
- Precipitation Ammonium Sulphate
- Specificity
This Mouse IgG2b Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
This antibody clone is manufactured by Abcam.
ab221850 is a PBS only buffer formulated version of Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control ab170192.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody's host species, isotype, and possible conjugation.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free (ab221850), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-bsa-and-azide-free-ab221850--flow-cytometry-img71328.jpg "Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free (ab221850)")
Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free (ab221850)
This flow cytometry data was generated using the same antibody clone under a different buffer format (cat# Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control ab170192).
Flow cytometry experiments with 4% PFA fixed Hela (human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast)were performed with the IgG2b Isotype Control Antibody (red) and no primary antibody negative control (black). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody.
The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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