Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control Cited in 10 publications.
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![Flow Cytometry - Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (AB170191), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191--flow-cytometry-img434623.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (AB170191)")
Publications
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- PloS one 18:e02821762023Deep learning neural network image analysis of immunohistochemical protein expression reveals a significantly reduced expression of biglycan in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Paula Thiesen,Bruna Mielczarski,Ricardo Francalacci SavarisPubMed 36972253
- NPJ Regenerative medicine 6:772021Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrank A Petrigliano,Nancy Q Liu,Siyoung Lee,Jade Tassey,Arijita Sarkar,Yucheng Lin,Liangliang Li,Yifan Yu,Dawei Geng,Jiankang Zhang,Ruzanna Shkhyan,Jacob Bogdanov,Ben Van Handel,Gabriel B Ferguson,Youngjoo Lee,Svenja Hinderer,Kuo-Chang Tseng,Aaron Kavanaugh,J Gage Crump,April D Pyle,Katja Schenke-Layland,Fabrizio Billi,Liming Wang,Jay Lieberman,Mark Hurtig,Denis EvseenkoPubMed 34815400
- Stem cell reviews and reports 17:1053-10672021Off-the-Shelf, Immune-Compatible Human Embryonic Stem Cells Generated Via CRISPR-Mediated Genome Editing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnie Kim,Kun-Gu Lee,Yeongbeen Kwon,Kang-In Lee,Heung-Mo Yang,Omer Habib,Jihun Kim,Sang-Tae Kim,Sung Joo Kim,Jin-Soo Kim,Dong-Youn HwangPubMed 33423156
- Aging 12:18274-182962020MSC-derived exosomes promote recovery from traumatic brain injury via microglia/macrophages in rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunfei Chen et. al.PubMed 32966240
- Scientific reports 9:127922019Flightless I exacerbation of inflammatory responses contributes to increased colonic damage in a mouse model of dextran sulphate sodium-induced ulcerative colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZ Kopecki et. al.PubMed 31488864
- Molecular cell 73:314-324.e42018The Pat1-Lsm Complex Stabilizes ATG mRNA during Nitrogen Starvation-Induced Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDamián Gatica,Guowu Hu,Xu Liu,Nannan Zhang,Peter R Williamson,Daniel J KlionskyPubMed 30527663
- Molecular imaging and biology 21:473-4812018SPECT/CT Imaging of Mycobacterium tuberculosis Infection with [I]anti-C3d mAb.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCatherine A Foss,Liudmila Kulik,Alvaro A Ordonez,Sanjay K Jain,V Michael Holers,Joshua M Thurman,Martin G PomperPubMed 29998399
- The international journal of biochemistry & cell b 100:22-292018Inhibitory effect of microRNA-154 targeting WHSC1 on cell proliferation of human skin squamous cell carcinoma through mediating the P53 signaling pathway.
- Stem cell reports 7:454-4702016G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis.
- Biomacromolecules 17:1280-912016Glycosaminoglycan-Mimetic Signals Direct the Osteo/Chondrogenic Differentiation of Mesenchymal Stem Cells in a Three-Dimensional Peptide Nanofiber Extracellular Matrix Mimetic Environment.
Key facts
- Isotype
IgG2c
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Expected | Dilution info 1 µg/mL | Notes - |
Application Flow Cyt | Reactivity Expected | Dilution info 1 µg/mL | Notes - |
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Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control Cited in 10 publications.
Key facts
- Isotype
IgG2c
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
- Clone number
18C8BC7AD10
- Purification technique
Precipitation Ammonium Sulphate
- Specificity
This Mouse IgG2c Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody's host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product was previously marketed under the MitoSciences sub-brand.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191), expandable thumbnail](https://content.abcam.com/products/images/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191--flow-cytometry-img434623.jpg "Flow Cytometry - Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191)")
Flow Cytometry - Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191)
Flow cytometry staining of C57 BL/6 mouse bone marrow cells with Anti-Ly6g antibody [1A8]- mouse IgG2c (Chimeric)- Low endotoxin, Azide free ab320708 (right) or Mouse IgG2c, kappa monoclonal (ab170191), isotype control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10%rat serum 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody Anti-Ly6g antibody [1A8]- mouse IgG2c (Chimeric)- Low endotoxin, Azide free ab320708 or Mouse IgG2c, kappa monoclonal (ab170191), isotype control (1x 10e6 in 100 µl at 1.0 μg/ml) for 30min on ice. The cells were simultaneously stained with APC anti-mouse Ly-6C Antibody.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.
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