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AB280756

Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control

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(1 Publication)

Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control Suitable for Flow Cyt (Intra), ICC/IF and reacts with samples. Cited in 1 publication.
6 Images
Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (AB280756)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (AB280756)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast, Left) / PC-12 (Rat adrenal gland pheochromocytoma, Right) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1μg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (AB280756)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (AB280756)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1μg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (AB280756)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (AB280756)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Confocal image showing no staining in PC-12 cells.

Negative control 1 : ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (AB280756)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (AB280756)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Confocal image showing no staining in MOLT-4 cells.

Negative control 1 : ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (AB280756)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (AB280756)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Confocal image showing no staining in NIH/3T3 cells.

Negative control 1 : ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (AB280756)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (AB280756)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1μg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

R313-MouseIgG2c

Isotype

IgG2c

Carrier free

No

Applications

Flow Cyt (Intra), ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "IHCP-species-checked": "", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/80", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 15:1399632 PubMed39282564

2024

Exendin-4-enriched exosomes from hUCMSCs alleviate diabetic nephropathy via gut microbiota and immune modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Wang,Aihua Liang,Jukai Huang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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