Mouse IgG2c monoclonal [R313-MouseIgG2c] - Isotype control - Low endotoxin, Azide free Suitable for Flow Cyt (Intra), ICC/IF and reacts with samples.
IgG2c
Mouse
Constituents: 100% PBS
Liquid
Monoclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application IHC-P | Reactivity Not expected | Dilution info - | Notes - |
Application Flow Cyt (Intra) | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info - | Notes - |
Mouse IgG2c monoclonal [R313-MouseIgG2c] - Isotype control - Low endotoxin, Azide free Suitable for Flow Cyt (Intra), ICC/IF and reacts with samples.
IgG2c
Mouse
Constituents: 100% PBS
Liquid
Monoclonal
Yes
R313-MouseIgG2c
Ion exchange chromatography
Blue Ice
+4°C
+4°C
Do Not Freeze
ab280757 is the carrier-free version of Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab280757 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This image was produced using Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756, the same clone but in a different formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling Mouse IgG2c with Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756 at 1/80 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in MOLT-4 cells.
Negative control 1: Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756 at a 1/80 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at a 1/200 dilution.
Negative control 2: Anti-beta Tubulin antibody [EPR16774] ab179513 at a 1/200 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 at a 1/1000 dilution.
This image was produced using Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756, the same clone but in a different formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labelling Mouse IgG2c with Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756 at 1/1000 dilution (0.1μg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody.
This image was produced using Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756, the same clone but in a different formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Mouse IgG2c with Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756 at 1/80 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in NIH/3T3 cells.
Negative control 1: Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756 at a 1/80 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at a 1/200 dilution.
Negative control 2: Anti-beta Tubulin antibody [EPR16774] ab179513 at a 1/200 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 at a 1/1000 dilution.
This image was produced using Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756, the same clone but in a different formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast, Left) / PC-12 (Rat adrenal gland pheochromocytoma, Right) cells labelling Mouse IgG2c with Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756 at 1/1000 dilution (0.1μg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody.
This image was produced using Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756, the same clone but in a different formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NPC-12 cells labelling Mouse IgG2c with Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756 at 1/80 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in PC-12 cells.
Negative control 1: Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control ab280756 at a 1/80 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at a 1/200 dilution.
Negative control 2: Anti-beta Tubulin antibody [EPR16774] ab179513 at a 1/200 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 at a 1/1000 dilution.
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