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Rabbit Recombinant Monoclonal IGHM antibody. Suitable for ICC, IP, Flow Cyt, ELISA, IHC-P and reacts with Mouse, Recombinant fragment, Recombinant full length protein - Mouse samples. Cited in 3 publications. Immunogen corresponding to Full Length Protein corresponding to Mouse Immunoglobulin heavy constant mu.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Full Length Protein corresponding to Mouse Immunoglobulin heavy constant mu. The exact immunogen used to generate this antibody is proprietary information. Database link P01872
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIPFlow CytELISAIHC-P
Mouse
Expected
Expected
Expected
Predicted
Expected
Recombinant fragment
Not recommended
Expected
Not recommended
Not recommended
Not recommended
Recombinant full length protein - Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment, Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Recombinant fragment, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species

Recombinant fragment, Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Mouse

Dilution info

0.005-0.2 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment, Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Target data

Function

IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions (By similarity).

Alternative names

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Rabbit Recombinant Monoclonal IGHM antibody. Suitable for ICC, IP, Flow Cyt, ELISA, IHC-P and reacts with Mouse, Recombinant fragment, Recombinant full length protein - Mouse samples. Cited in 3 publications. Immunogen corresponding to Full Length Protein corresponding to Mouse Immunoglobulin heavy constant mu.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Full Length Protein corresponding to Mouse Immunoglobulin heavy constant mu. The exact immunogen used to generate this antibody is proprietary information. Database link P01872
Clone number

RM109

Purification technique

Affinity purification Protein A

Specificity

This antibody reacts to mouse IgM. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The Fc region of RM109 has been engineered to eliminate the binding of Fc receptor.

Concentration
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Purification notes

ab190369 was purified from an animal origin–free and protein-free culture supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

Biological function summary

Mouse IgM acts as a pivotal component in the body's defense mechanisms against infections. It forms part of a complex known as the complement system particularly activating the classic complement pathway. This activation facilitates opsonization where pathogens are marked for ingestion and destruction by macrophages and other immune cells. Alongside IgG antibodies Mouse IgM plays a vital role in immune surveillance and rapid response to foreign antigens although IgM is larger and less specialized compared to its IgG counterpart.

Activity summary

Mouse IgM also known as Immunoglobulin M is an essential type of antibody found in mice with a molecular mass of approximately 970 kDa. This antibody is mainly expressed in the blood and lymphatic fluid and is the first antibody produced by B cells upon exposure to an antigen. The pentameric form of Mouse IgM has ten antigen-binding sites which enhances its ability to neutralize pathogens effectively and forms the foundation of the initial immune response.

Pathways

Mouse IgM is critically connected to the classical complement activation pathway and the B cell signaling pathway. In the complement cascade it interacts with C1 complex beginning the process of pathogen elimination. In the context of B cell receptor-mediated signaling IgM antibodies serve as receptors on naive B cells synergizing with proteins such as Ig-alpha and Ig-beta to initiate signaling cascades essential for B cell maturation and differentiation. These pathways ensure the development of a robust adaptive immune response.

Associated diseases and disorders

Dysfunction or deficiency in Mouse IgM can lead to conditions such as immunodeficiencies and autoimmune disorders. For instance a lack of functional IgM results in hyper-IgM syndrome where there is an elevated level of IgG but low levels of IgM impacting the body's ability to fight infections. Additionally systemic lupus erythematosus an autoimmune disease is often associated with abnormal IgM activity. During this condition improper interaction with IgG may exacerbate autoantibody production highlighting the complex relationship between IgM and other immunoglobulins in managing immune health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • ELISA - Anti-Mouse IgM antibody [RM109] (ab190369), expandable thumbnail

    ELISA - Anti-Mouse IgM antibody [RM109] (ab190369)

    A titer ELISA of mouse IgM. The plate was coated with different amounts of mouse IgM. A serial dilution of ab190369 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA - Anti-Mouse IgM antibody [RM109] (ab190369), expandable thumbnail

    ELISA - Anti-Mouse IgM antibody [RM109] (ab190369)

    ELISA of mouse immunoglobulins shows ab190369 reacted to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab190369 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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