Mouse Recombinant Monoclonal M1 antibody. Suitable for WB, ICC/IF, I-ELISA and reacts with Transfected cell lysate - Influenza A virus (A/Wilson-Smith/1933(H1N1)), Transfected cell line - Influenza A virus (A/Wilson-Smith/1933(H1N1)), Recombinant fragment - Influenza A virus (A/Wilson-Smith/1933(H1N1)) samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (AB322959), expandable thumbnail](https://content.abcam.com/products/images/mouse-influenza-a-virus-m1-protein-antibody-1g1a12-ab322959--immunocytochemistry-immunofluorescence-img444455.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (AB322959)")
![Indirect ELISA - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (AB322959), expandable thumbnail](https://content.abcam.com/products/images/mouse-influenza-a-virus-m1-protein-antibody-1g1a12-ab322959--indirect-elisa-img444459.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (AB322959)")
![Western blot - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (AB322959), expandable thumbnail](https://content.abcam.com/products/images/mouse-influenza-a-virus-m1-protein-antibody-1g1a12-ab322959--western-blot-img444440.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (AB322959)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | I-ELISA | |
|---|---|---|---|
| Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Predicted | Predicted | Predicted |
| Recombinant fragment - Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Not recommended | Not recommended | Tested |
| Transfected cell line - Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Not recommended | Tested | Not recommended |
| Transfected cell lysate - Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Influenza A virus (A/Wilson-Smith/1933(H1N1)), Recombinant fragment - Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Dilution info 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Influenza A virus (A/Wilson-Smith/1933(H1N1)), Recombinant fragment - Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Dilution info 125 ng/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Influenza A virus (A/Wilson-Smith/1933(H1N1)), Transfected cell line - Influenza A virus (A/Wilson-Smith/1933(H1N1)) | Dilution info - | Notes - |
Target data
Function
Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place. Determines the virion's shape: spherical or filamentous. Clinical isolates of influenza are characterized by the presence of significant proportion of filamentous virions, whereas after multiple passage on eggs or cell culture, virions have only spherical morphology. Filamentous virions are thought to be important to infect neighboring cells, and spherical virions more suited to spread through aerosol between hosts organisms.
Alternative names
Matrix protein 1, M1
Mouse Recombinant Monoclonal M1 antibody. Suitable for WB, ICC/IF, I-ELISA and reacts with Transfected cell lysate - Influenza A virus (A/Wilson-Smith/1933(H1N1)), Transfected cell line - Influenza A virus (A/Wilson-Smith/1933(H1N1)), Recombinant fragment - Influenza A virus (A/Wilson-Smith/1933(H1N1)) samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 1G1A12
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (ab322959), expandable thumbnail](https://content.abcam.com/products/images/mouse-influenza-a-virus-m1-protein-antibody-1g1a12-ab322959--immunocytochemistry-immunofluorescence-img444455.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (ab322959)")
Immunocytochemistry/ Immunofluorescence - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (ab322959)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 100% Methanol permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A virus M1 protein with ab322959 at 1/2000 (0.50 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 1000 2ug/ml dilution (Green).
Confocal image showing nuclear staining in 293T cells transfected with a Influenza A virus M1 protein expression vector containing a myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1: ab322959 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.
-ve control 2: Anti-Myc tag antibody ab9106 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
![Indirect ELISA - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (ab322959), expandable thumbnail](https://content.abcam.com/products/images/mouse-influenza-a-virus-m1-protein-antibody-1g1a12-ab322959--indirect-elisa-img444459.jpg "Indirect ELISA - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (ab322959)")
Indirect ELISA - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (ab322959)
Indirect ELISA analysis of ab322959 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at 1:1000 dilution dilution.
Antigen: Influenza A virus M1 protein.
Antigen concentration: 1000 ng/ml
![Western blot - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (ab322959), expandable thumbnail](https://content.abcam.com/products/images/mouse-influenza-a-virus-m1-protein-antibody-1g1a12-ab322959--western-blot-img444440.jpg "Western blot - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (ab322959)")
Western blot - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (ab322959)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (ab322959) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containi a myc-His-tag® whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a Influenza A virus M1 protein expression vector containi a myc-His-tag® whole cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 28 kDa, 124 kDa, 20-28 kDa
Exposure time: 1s
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