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AB322959

Anti-Mouse Influenza A virus M1 protein antibody [1G1A12]

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Mouse Recombinant Monoclonal M1 antibody. Suitable for WB, ICC/IF, I-ELISA and reacts with Transfected cell lysate - Influenza A virus (A/Wilson-Smith/1933(H1N1)), Transfected cell line - Influenza A virus (A/Wilson-Smith/1933(H1N1)), Recombinant fragment - Influenza A virus (A/Wilson-Smith/1933(H1N1)) samples.

View Alternative Names

Matrix protein 1, M1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (AB322959)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (AB322959)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 100% Methanol permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A virus M1 protein with ab322959 at 1/2000 (0.50 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a Influenza A virus M1 protein expression vector containing a myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab322959 at 1/2000 dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

-ve control 2 : ab9106 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Indirect ELISA - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (AB322959)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (AB322959)

Indirect ELISA analysis of ab322959 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at 1 : 1000 dilution dilution.

Antigen : Influenza A virus M1 protein.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (AB322959)
  • WB

Supplier Data

Western blot - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (AB322959)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (ab322959) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containi a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Influenza A virus M1 protein expression vector containi a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 28 kDa,124 kDa,20-28 kDa

false

Exposure time: 1s

  • Carrier free

    Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1G1A12

Isotype

IgG1

Carrier free

No

Reacts with

Influenza A virus (A/Wilson-Smith/1933(H1N1))

Applications

WB, ICC/IF, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A virus (A/Wilson-Smith/1933(H1N1))": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Influenza A virus (A/Wilson-Smith/1933(H1N1))": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "125 ng/mL", "IELISA-species-notes": "<p></p>" }, "Transfected cell line - Influenza A virus (A/Wilson-Smith/1933(H1N1))": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Transfected cell lysate - Influenza A virus (A/Wilson-Smith/1933(H1N1))": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place.. Determines the virion's shape : spherical or filamentous. Clinical isolates of influenza are characterized by the presence of significant proportion of filamentous virions, whereas after multiple passage on eggs or cell culture, virions have only spherical morphology. Filamentous virions are thought to be important to infect neighboring cells, and spherical virions more suited to spread through aerosol between hosts organisms.
See full target information M
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com