Rabbit Polyclonal MOV10 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 13 publications.
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- mBio 12:e01316212021MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Wang,Isabel Sola,Luis Enjuanes,Sonia ZuñigaPubMed 34517762
- Journal of visualized experiments : JoVE :2020Single-cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaven Shah,Shuiyun Lan,Maritza N Puray-Chavez,Dandan Liu,Philip R Tedbury,Stefan G SarafianosPubMed 33191939
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:9433-94492020Effect of P-body component Mov10 on HCV virus production and infectivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDandan Liu,Tanyaradzwa P Ndongwe,Maritza Puray-Chavez,Mary C Casey,Taisuke Izumi,Vinay K Pathak,Philip R Tedbury,Stefan G SarafianosPubMed 32496609
- Cell biology international 44:1671-16802020LINC00667 promotes the proliferation, migration, and pathological angiogenesis in non-small cell lung cancer through stabilizing VEGFA by EIF4A3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuizhen Yang,Wuchen Yang,Wanqing Dai,Yun Ma,Guojun ZhangPubMed 32281700
- The Journal of biological chemistry 294:19804-198132019The MOV10 helicase restricts hepatitis B virus replication by inhibiting viral reverse transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Liu et. al.PubMed 31722967
- Viruses 11:2019Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaritza N Puray-Chavez et. al.PubMed 31319455
- Molecular brain 12:652019NMDAR mediated translation at the synapse is regulated by MOV10 and FMRP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPreeti Madhav Kute et. al.PubMed 31291981
- BMC biology 17:392019Biological and RNA regulatory function of MOV10 in mammalian germ cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaiqiang Fu et. al.PubMed 31088452
- Cell 174:1537-1548.e292018Uridylation by TUT4/7 Restricts Retrotransposition of Human LINE-1s.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZbigniew Warkocki et. al.PubMed 30122351
- Oncogenesis 4:e1542015Regulation of lipid synthesis by the RNA helicase Mov10 controls Wnt5a production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesW Wang et. al.PubMed 26029828
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Ferret | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 6 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Gorilla, Orangutan, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Gorilla, Orangutan, Rhesus monkey | Dilution info - | Notes - |
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Target data
Function
5' to 3' RNA helicase that is involved in a number of cellular roles ranging from mRNA metabolism and translation, modulation of viral infectivity, inhibition of retrotransposition, or regulation of synaptic transmission (PubMed:23093941). Plays an important role in innate antiviral immunity by promoting type I interferon production (PubMed:27016603, PubMed:27974568, PubMed:35157734). Mechanistically, specifically uses IKKepsilon/IKBKE as the mediator kinase for IRF3 activation (PubMed:27016603, PubMed:35157734). Blocks HIV-1 virus replication at a post-entry step (PubMed:20215113). Counteracts HIV-1 Vif-mediated degradation of APOBEC3G through its helicase activity by interfering with the ubiquitin-proteasome pathway (PubMed:29258557). Inhibits also hepatitis B virus/HBV replication by interacting with HBV RNA and thereby inhibiting the early step of viral reverse transcription (PubMed:31722967). Contributes to UPF1 mRNA target degradation by translocation along 3' UTRs (PubMed:24726324). Required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed:16289642, PubMed:17507929, PubMed:22791714). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (PubMed:25464849). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1 (PubMed:23093941, PubMed:30122351). Facilitates LINE-1 uridylation by TUT4 and TUT7 (PubMed:30122351). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol (By similarity). May function as a messenger ribonucleoprotein (mRNP) clearance factor (PubMed:24726324). (Microbial infection) Required for RNA-directed transcription and replication of the human hepatitis delta virus (HDV). Interacts with small capped HDV RNAs derived from genomic hairpin structures that mark the initiation sites of RNA-dependent HDV RNA transcription.
Alternative names
KIAA1631, MOV10, Helicase MOV-10, Armitage homolog, Moloney leukemia virus 10 protein
Recommended products
Rabbit Polyclonal MOV10 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 13 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibody was affinity purified using an epitope specific to MOV10 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Mov10 also known as Mov10 RNA helicase is an ATP-dependent helicase involved in RNA silencing mechanisms. It belongs to the UPF1-like helicase family and has a molecular weight of approximately 106 kDa. Mov10 localizes in the cytoplasm and also associates with the Argonaute protein in processing (P) bodies. It is expressed in many tissues with notable presence in the brain heart and testis. Mov10 contributes to RNA regulation by unwinding RNA structures facilitating degradation or inhibition.
- Biological function summary
Mov10 plays roles in RNA interference (RNAi) processes. It forms complexes including interactions with enzymes like Dicer and Argonaute proteins aiding in microRNA (miRNA) and small interfering RNA (siRNA) pathways. These complexes regulate gene expression by degrading or repressing target mRNAs. Mov10 influences translational repression mechanisms by modulating the availability of specific mRNA transcripts which affects cellular protein synthesis.
- Pathways
Mov10 is instrumental in RNA processing and regulation pathways. It notably interacts with the RNA-induced silencing complex (RISC) and influences the mRNA surveillance pathway. Through these pathways Mov10 works alongside the proteins Dicer and Argonaute to mediate gene silencing and mRNA decay. These interactions play a part in modulating gene expression responses under various cellular conditions and stresses.
- Associated diseases and disorders
Research links Mov10 to neurodegenerative diseases and certain cancers. In Alzheimer's disease Mov10 interacts with proteins such as amyloid precursor protein (APP) influencing amyloid-beta peptide accumulation a known pathogenic process. Additionally Mov10 overexpression is observed in hepatocellular carcinoma associating with disrupted mRNA regulation and cancer cell proliferation. Understanding Mov10's role in these disorders could highlight potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Mov10 antibody (ab80613)
Lane 1: Western blot - Anti-Mov10 antibody (ab80613) at 1 µg/mL
Lanes 2 - 5: Western blot - Anti-Mov10 antibody (ab80613) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Lane 5: NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Observed band size: 114 kDa
Exposure time: 3min
Immunoprecipitation - Anti-Mov10 antibody (ab80613)
Immunoprecipitation of HeLa whole cell lysate using ab80613 at 6μg/mg (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80613 at 1μg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.All lanes: Immunoprecipitation - Anti-Mov10 antibody (ab80613)
Predicted band size: 114 kDa
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