AB80613

Anti-Mov10 antibody

Name: Anti-Mov10 antibody (ab80613) | Abcam
Brand: Abcam Limited
SKU: AB80613
Price: 545 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(17 Publications)

Rabbit Polyclonal MOV10 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 17 publications.

View Alternative Names

KIAA1631, MOV10, Helicase MOV-10, Armitage homolog, Moloney leukemia virus 10 protein

2 Images

Unknown

Immunoprecipitation - Anti-Mov10 antibody (AB80613)

Immunoprecipitation of HeLa whole cell lysate using ab80613 at 6μg/mg (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80613 at 1μg/ml.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Mov10 antibody (ab80613)

Predicted band size: 114 kDa

false

Unknown

Western blot - Anti-Mov10 antibody (AB80613)

Lane 1:

Western blot - Anti-Mov10 antibody (ab80613) at 1 µg/mL

Lanes 2 - 5:

Western blot - Anti-Mov10 antibody (ab80613) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 114 kDa

Observed band size: 114 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "6 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to MOV10 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mov10 also known as Mov10 RNA helicase is an ATP-dependent helicase involved in RNA silencing mechanisms. It belongs to the UPF1-like helicase family and has a molecular weight of approximately 106 kDa. Mov10 localizes in the cytoplasm and also associates with the Argonaute protein in processing (P) bodies. It is expressed in many tissues with notable presence in the brain heart and testis. Mov10 contributes to RNA regulation by unwinding RNA structures facilitating degradation or inhibition.

Biological function summary

Mov10 plays roles in RNA interference (RNAi) processes. It forms complexes including interactions with enzymes like Dicer and Argonaute proteins aiding in microRNA (miRNA) and small interfering RNA (siRNA) pathways. These complexes regulate gene expression by degrading or repressing target mRNAs. Mov10 influences translational repression mechanisms by modulating the availability of specific mRNA transcripts which affects cellular protein synthesis.

Pathways

Mov10 is instrumental in RNA processing and regulation pathways. It notably interacts with the RNA-induced silencing complex (RISC) and influences the mRNA surveillance pathway. Through these pathways Mov10 works alongside the proteins Dicer and Argonaute to mediate gene silencing and mRNA decay. These interactions play a part in modulating gene expression responses under various cellular conditions and stresses.

Research links Mov10 to neurodegenerative diseases and certain cancers. In Alzheimer's disease Mov10 interacts with proteins such as amyloid precursor protein (APP) influencing amyloid-beta peptide accumulation a known pathogenic process. Additionally Mov10 overexpression is observed in hepatocellular carcinoma associating with disrupted mRNA regulation and cancer cell proliferation. Understanding Mov10's role in these disorders could highlight potential therapeutic targets.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

5' to 3' RNA helicase that is involved in a number of cellular roles ranging from mRNA metabolism and translation, modulation of viral infectivity, inhibition of retrotransposition, or regulation of synaptic transmission (PubMed : 23093941). Plays an important role in innate antiviral immunity by promoting type I interferon production (PubMed : 27016603, PubMed : 27974568, PubMed : 35157734). Mechanistically, specifically uses IKKepsilon/IKBKE as the mediator kinase for IRF3 activation (PubMed : 27016603, PubMed : 35157734). Blocks HIV-1 virus replication at a post-entry step (PubMed : 20215113). Counteracts HIV-1 Vif-mediated degradation of APOBEC3G through its helicase activity by interfering with the ubiquitin-proteasome pathway (PubMed : 29258557). Inhibits also hepatitis B virus/HBV replication by interacting with HBV RNA and thereby inhibiting the early step of viral reverse transcription (PubMed : 31722967). Contributes to UPF1 mRNA target degradation by translocation along 3' UTRs (PubMed : 24726324). Required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed : 16289642, PubMed : 17507929, PubMed : 22791714). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (PubMed : 25464849). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1 (PubMed : 23093941, PubMed : 30122351). Facilitates LINE-1 uridylation by TUT4 and TUT7 (PubMed : 30122351). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development : suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol (By similarity). May function as a messenger ribonucleoprotein (mRNP) clearance factor (PubMed : 24726324).. (Microbial infection) Required for RNA-directed transcription and replication of the human hepatitis delta virus (HDV). Interacts with small capped HDV RNAs derived from genomic hairpin structures that mark the initiation sites of RNA-dependent HDV RNA transcription.

See full target information MOV10

style

left-column

Publications (17)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:110418 PubMed40570961

2025

Functional investigation of the RNA helicase MOV10 with respect to its interplay with factors involved in nonsense-mediated mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Guangpu Xue,Gabriel P Faber,Lea S Pommerening,Megha Mallick,Aditi Gupta,Markus C Wahl,Yaron Shav-Tal,Sutapa Chakrabarti

Molecular medicine reports 31: PubMed39450567

2024

S100A16 stabilizes the ITGA3‑mediated ECM‑receptor interaction pathway to drive the malignant properties of lung adenocarcinoma cells via binding MOV10.

Applications

Unspecified application

Species

Unspecified reactive species

Lianren Yang,Ajuan Shen,Rujun Wang,Zhihui Zheng

Mobile DNA 15:14 PubMed38937837

2024

Subcellular location of L1 retrotransposon-encoded ORF1p, reverse transcription products, and DNA sensors in lupus granulocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Moadab,Sepideh Sohrabi,Xiaoxing Wang,Rayan Najjar,Justina C Wolters,Hua Jiang,Wenyan Miao,Donna Romero,Dennis M Zaller,Megan Tran,Alison Bays,Martin S Taylor,Rosana Kapeller,John LaCava,Tomas Mustelin

Science advances 8:eabq2945 PubMed36001654

2022

RNA binding protein RBM46 regulates mitotic-to-meiotic transition in spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Baomei Qian,Yang Li,Ruoyu Yan,Shenglin Han,Zhiwen Bu,Jie Gong,Bangjin Zheng,Zihan Yuan,Sen Ren,Qing He,Jinwen Zhang,Chen Xu,Ruilin Wang,Zheng Sun,Mingyan Lin,Jian Zhou,Lan Ye

mBio 12:e0131621 PubMed34517762

2021

MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Isabel Sola,Luis Enjuanes,Sonia Zuñiga

Journal of visualized experiments : JoVE : PubMed33191939

2020

Single-cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Raven Shah,Shuiyun Lan,Maritza N Puray-Chavez,Dandan Liu,Philip R Tedbury,Stefan G Sarafianos

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:9433-9449 PubMed32496609

2020

Effect of P-body component Mov10 on HCV virus production and infectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Liu,Tanyaradzwa P Ndongwe,Maritza Puray-Chavez,Mary C Casey,Taisuke Izumi,Vinay K Pathak,Philip R Tedbury,Stefan G Sarafianos

Cell biology international 44:1671-1680 PubMed32281700

2020

LINC00667 promotes the proliferation, migration, and pathological angiogenesis in non-small cell lung cancer through stabilizing VEGFA by EIF4A3.

Applications

Unspecified application

Species

Unspecified reactive species

Huizhen Yang,Wuchen Yang,Wanqing Dai,Yun Ma,Guojun Zhang

The Journal of biological chemistry 294:19804-19813 PubMed31722967

2019

The MOV10 helicase restricts hepatitis B virus replication by inhibiting viral reverse transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Liu,Qingsong Sun,Yong Liu,Shan Cen,Quan Zhang

Viruses 11: PubMed31319455

2019

Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Maritza N Puray-Chavez,Mahmoud H Farghali,Vincent Yapo,Andrew D Huber,Dandan Liu,Tanyaradzwa P Ndongwe,Mary C Casey,Thomas G Laughlin,Mark Hannink,Philip R Tedbury,Stefan G Sarafianos

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mov10 antibody

Immunoprecipitation - Anti-Mov10 antibody (AB80613)

Western blot - Anti-Mov10 antibody (AB80613)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise