AB189919

Anti-Mov10 antibody [EPR14478]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal MOV10 antibody. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 5 publications.

View Alternative Names

KIAA1631, MOV10, Helicase MOV-10, Armitage homolog, Moloney leukemia virus 10 protein

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mov10 antibody [EPR14478] (AB189919)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mov10 antibody [EPR14478] (AB189919)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Mov10 with ab189919 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Mov10 antibody [EPR14478] (AB189919)

All lanes:

Western blot - Anti-Mov10 antibody [EPR14478] (ab189919) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 114 kDa

Observed band size: 114 kDa

false

Supplier Data

Western blot - Anti-Mov10 antibody [EPR14478] (AB189919)

All lanes:

Western blot - Anti-Mov10 antibody [EPR14478] (ab189919) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

NIH/3T3 cell lysate at 10 µg

Lane 3:

Raw264.7 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 114 kDa

Observed band size: 114 kDa

false

Supplier Data

Western blot - Anti-Mov10 antibody [EPR14478] (AB189919)

All lanes:

Western blot - Anti-Mov10 antibody [EPR14478] (ab189919) at 1/5000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 114 kDa

Observed band size: 114 kDa

false

Carrier free

Anti-Mov10 antibody [EPR14478] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14478

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mov10 also known as Mov10 RNA helicase is an ATP-dependent helicase involved in RNA silencing mechanisms. It belongs to the UPF1-like helicase family and has a molecular weight of approximately 106 kDa. Mov10 localizes in the cytoplasm and also associates with the Argonaute protein in processing (P) bodies. It is expressed in many tissues with notable presence in the brain heart and testis. Mov10 contributes to RNA regulation by unwinding RNA structures facilitating degradation or inhibition.

Biological function summary

Mov10 plays roles in RNA interference (RNAi) processes. It forms complexes including interactions with enzymes like Dicer and Argonaute proteins aiding in microRNA (miRNA) and small interfering RNA (siRNA) pathways. These complexes regulate gene expression by degrading or repressing target mRNAs. Mov10 influences translational repression mechanisms by modulating the availability of specific mRNA transcripts which affects cellular protein synthesis.

Pathways

Mov10 is instrumental in RNA processing and regulation pathways. It notably interacts with the RNA-induced silencing complex (RISC) and influences the mRNA surveillance pathway. Through these pathways Mov10 works alongside the proteins Dicer and Argonaute to mediate gene silencing and mRNA decay. These interactions play a part in modulating gene expression responses under various cellular conditions and stresses.

Research links Mov10 to neurodegenerative diseases and certain cancers. In Alzheimer's disease Mov10 interacts with proteins such as amyloid precursor protein (APP) influencing amyloid-beta peptide accumulation a known pathogenic process. Additionally Mov10 overexpression is observed in hepatocellular carcinoma associating with disrupted mRNA regulation and cancer cell proliferation. Understanding Mov10's role in these disorders could highlight potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

5' to 3' RNA helicase that is involved in a number of cellular roles ranging from mRNA metabolism and translation, modulation of viral infectivity, inhibition of retrotransposition, or regulation of synaptic transmission (PubMed : 23093941). Plays an important role in innate antiviral immunity by promoting type I interferon production (PubMed : 27016603, PubMed : 27974568, PubMed : 35157734). Mechanistically, specifically uses IKKepsilon/IKBKE as the mediator kinase for IRF3 activation (PubMed : 27016603, PubMed : 35157734). Blocks HIV-1 virus replication at a post-entry step (PubMed : 20215113). Counteracts HIV-1 Vif-mediated degradation of APOBEC3G through its helicase activity by interfering with the ubiquitin-proteasome pathway (PubMed : 29258557). Also inhibits hepatitis B virus/HBV replication by interacting with HBV RNA and thereby inhibiting the early step of viral reverse transcription (PubMed : 31722967). Contributes to UPF1 mRNA target degradation by translocation along 3' UTRs (PubMed : 24726324). Required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed : 16289642, PubMed : 17507929, PubMed : 22791714). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (PubMed : 25464849). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1 (PubMed : 23093941, PubMed : 30122351). Facilitates LINE-1 uridylation by TUT4 and TUT7 (PubMed : 30122351). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development : suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol (By similarity). May function as a messenger ribonucleoprotein (mRNP) clearance factor (PubMed : 24726324).. (Microbial infection) Required for RNA-directed transcription and replication of the human hepatitis delta virus (HDV). Interacts with small capped HDV RNAs derived from genomic hairpin structures that mark the initiation sites of RNA-dependent HDV RNA transcription.

See full target information MOV10

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 31: PubMed39450567

2024

S100A16 stabilizes the ITGA3‑mediated ECM‑receptor interaction pathway to drive the malignant properties of lung adenocarcinoma cells via binding MOV10.

Applications

Unspecified application

Species

Unspecified reactive species

Lianren Yang,Ajuan Shen,Rujun Wang,Zhihui Zheng

Pharmacology 108:344-358 PubMed37231999

2023

Melatonin Regulates lncRNA NEAT1/miR-138-5p/HIF-1α Axis through MOV10 to Affect Acid-Related Esophageal Epithelial Cell Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Su,Wenjie Li,Danping Zhang,Hong Zhu

Cell reports 42:112028 PubMed36848233

2023

SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Lihui Wang,Yue Xu,Sijung Yun,Quan Yuan,Prasanna Satpute-Krishnan,Yihong Ye

PLoS pathogens 18:e1010301 PubMed35157734

2022

Host MOV10 is induced to restrict herpes simplex virus 1 lytic infection by promoting type I interferon response.

Applications

Unspecified application

Species

Unspecified reactive species

Xiyuan Yang,Ze Xiang,Zeyu Sun,Feiyang Ji,Keyi Ren,Dongli Pan

The EMBO journal 40:e105492 PubMed33709510

2021

Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Lorena Martin-Jaular,Nathalie Nevo,Julia P Schessner,Mercedes Tkach,Mabel Jouve,Florent Dingli,Damarys Loew,Kenneth W Witwer,Matias Ostrowski,Georg H H Borner,Clotilde Théry

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mov10 antibody [EPR14478]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mov10 antibody [EPR14478] (AB189919)

Western blot - Anti-Mov10 antibody [EPR14478] (AB189919)

Western blot - Anti-Mov10 antibody [EPR14478] (AB189919)

Western blot - Anti-Mov10 antibody [EPR14478] (AB189919)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise