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AB236977

Anti-MOV10L1/Champ antibody

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(1 Publication)

Rabbit Polyclonal MOV10L1/Champ antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RNA helicase Mov10l1 aa 300-450.

View Alternative Names

RNA helicase Mov10l1, Moloney leukemia virus 10-like protein 1, MOV10-like protein 1, MOV10L1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOV10L1/Champ antibody (AB236977)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOV10L1/Champ antibody (AB236977)

Paraffin-embedded human liver cancer tissue stained for MOV10L1/Champ using ab236977 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOV10L1/Champ antibody (AB236977)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOV10L1/Champ antibody (AB236977)

Paraffin-embedded human breast cancer tissue stained for MOV10L1/Champ using ab236977 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-MOV10L1/Champ antibody (AB236977)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MOV10L1/Champ antibody (AB236977)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for MOV10L1/Champ (green) using ab236977 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

Western blot - Anti-MOV10L1/Champ antibody (AB236977)
  • WB

Supplier Data

Western blot - Anti-MOV10L1/Champ antibody (AB236977)

All lanes:

Western blot - Anti-MOV10L1/Champ antibody (ab236977) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 135 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human RNA helicase Mov10l1 aa 300-450. The exact immunogen used to generate this antibody is proprietary information.

Q9BXT6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MOV10L1 also known as Champ is a protein that acts as a RNA helicase with well-characterized unwinding properties. It has an approximate molecular mass of 130 kDa. MOV10L1 is expressed prominently in the testis specifically in germ cells during spermatogenesis. The expression of this protein is regulated tightly indicating its involvement in specific cellular processes related to fertility.
Biological function summary

MOV10L1 participates actively in the piRNA pathway which plays a role in silencing transposable elements in the germline. It forms part of complexes with Piwi proteins which are essential for the maturation of piRNAs and the maintenance of genome integrity in the germ cells. MOV10L1 ensures proper germ cell development by governing piRNA biogenesis. This process indicates its importance in protecting the genetic material from potential transposon-induced damage.

Pathways

MOV10L1 integrates into the piRNA metabolic pathway a vital part of post-transcriptional gene regulation. Piwi-interacting proteins such as PIWIL1 and PIWIL2 work tightly alongside MOV10L1. In this pathway MOV10L1 assists in the stabilization and processing of piRNAs therefore ensuring effective silencing of transposable elements. This function places MOV10L1 at a critical junction of gene regulation and genome safeguarding during germ cell differentiation.

MOV10L1 is implicated notably in infertility due to its role in spermatogenesis. Deficiency or dysfunction of MOV10L1 links to spermatogenic failure resulting in compromised male fertility. Furthermore connections between MOV10L1 and factors like DAZL and MVH both related to germ cell function further highlight its relevance in reproductive health. Understanding the disruptions in MOV10L1 function can lead to insights into certain infertility disorders potentially guiding therapeutic avenues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the primary piRNA metabolic process. Specifically binds to piRNA precursors and promotes the generation of intermediate piRNA processing fragments that are subsequently loaded to Piwi proteins. Acts via its ATP-dependent RNA helicase activity : displays 5'-3' RNA unwinding activity and probably mediates unwinding and funneling of single-stranded piRNA precursor transcripts to the endonuclease that catalyzes the first cleavage step of piRNA processing to generate piRNA intermediate fragments that are subsequently loaded to Piwi proteins.
See full target information RNA helicase Mov10l1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 12:9549-9584 PubMed32454462

2020

MiR-1908/EXO1 and MiR-203a/FOS, regulated by scd1, are associated with fracture risk and bone health in postmenopausal diabetic women.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Sheng Chen,Xue-Ran Kang,Zi-Hui Zhou,Jiang Yang,Qi Xin,Chen-Ting Ying,Yun-Peng Zhang,Jie Tao
View all publications
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