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AB74871

Anti-MPC1 antibody

1

(1 Review)

|

(10 Publications)

Rabbit Polyclonal MPC1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human MPC1.

View Alternative Names

BRP44L, CGI-129, HSPC040, PNAS-115, MPC1, Mitochondrial pyruvate carrier 1, Brain protein 44-like protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MPC1 antibody (AB74871)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MPC1 antibody (AB74871)

ab74871, at a 1/500 dilution, staining MPC1 in A549 cells by Immunofluorescence in the absence (left image) or presence (right image) of the immunizing peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPC1 antibody (AB74871)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPC1 antibody (AB74871)

ab74871, at a 1/50 dilution, staining MPC1 in paraffin embedded human heart tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.

Western blot - Anti-MPC1 antibody (AB74871)
  • WB

Unknown

Western blot - Anti-MPC1 antibody (AB74871)

All lanes:

Western blot - Anti-MPC1 antibody (ab74871) at 1/500 dilution

Lane 1:

extracts from COLO cells at 20 µg

Lane 2:

extracts from HeLa cells at 20 µg

Lane 3:

extracts from COLO cells at 20 µg with immunizing peptide

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human MPC1. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5U8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mitochondrial pyruvate carrier 1 (MPC1) also known as BRP44L is part of the MPC complex that facilitates pyruvate transport into mitochondria. This protein has a mass of approximately 15 kDa. MPC1 is expressed in various tissues with high metabolic demand including the liver heart and muscle. These tissues rely on effective pyruvate metabolism for energy production which highlights MPC1’s importance in cellular respiration and metabolic processes.
Biological function summary

MPC1 forms an important component of the MPC complex functioning alongside MPC2 to enable pyruvate import into mitochondria where it aids in energy production through the Krebs cycle. This import is essential for maintaining the balance of cellular metabolism and supporting ATP generation. The MPC1 complex has garnered interest due to its involvement in mitochondrial function and energy homeostasis important for cellular metabolism.

Pathways

MPC1 involvement centers on the integration of glycolysis and the tricarboxylic acid cycle. This protein provides a critical link for pyruvate conversion into acetyl-CoA influencing oxidative phosphorylation. MPC1 connects with other proteins like MPC2 forming a critical transport system. Alteration in the activity of these pathways can impact cellular energy levels and metabolic homeostasis highlighting its relevance in intermediary metabolism.

Mutations or dysfunctions in MPC1 have been associated with metabolic syndromes such as lactic acidosis and diabetes. The aberrant function of MPC1 in these conditions disrupts normal pyruvate metabolism leading to metabolic imbalance. In relation to diabetes MPC1’s interplay with insulin signaling pathways draws attention to its indirect role in the development of insulin resistance further connecting it to disorders involving energy dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates the uptake of pyruvate into mitochondria.
See full target information MPC1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1253 PubMed40601174

2025

Mitochondrial pyruvate carrier inhibits tumor growth by Bmi1 ubiquitination in renal cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Jian,Nana Hu,Rong He,Le He,He Xiao,Xingqiao Peng,Yang Peng,Yuxin Yang,Xiaoyan Dai,Dong Wang,Yan Feng,Nan Dai,Qian Chen

Technology in cancer research & treatment 23:15330338241282080 PubMed39360506

2024

Mitochondrial Pyruvate Carrier 1 as a Novel Prognostic Biomarker in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbo Zou,Yunfei Yin,Kai Xiong,Xuelian Luo,Zhongju Sun,Bijing Mao,Qichao Xie,Mei Tan,Rui Kong

EMBO reports 24:e56325 PubMed36794620

2023

METTL14 modulates glycolysis to inhibit colorectal tumorigenesis in p53-wild-type cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yichao Hou,Xintian Zhang,Han Yao,Lidan Hou,Qingwei Zhang,Enwei Tao,Xiaoqiang Zhu,Shanshan Jiang,Yimeng Ren,Xialu Hong,Shiyuan Lu,Xiaoxu Leng,Yile Xie,Yaqi Gao,Yu Liang,Ting Zhong,Bohan Long,Jing-Yuan Fang,Xiangjun Meng

The Journal of biological chemistry :101775 PubMed35257748

2022

Mitochondrial pyruvate carrier blockade results in decreased osteoclastogenesis and bone resorption via regulating mitochondrial energy production.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Guo,Hongjian Zhao,Haozhe Cheng,Honglei Kang,Yimin Dong,Renpeng Peng,Meipeng Zhu,Zhong Fang,Feng Li

Journal of immunology research 2020:8340329 PubMed32851100

2020

MPC1 Deficiency Promotes CRC Liver Metastasis via Facilitating Nuclear Translocation of -Catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Guang-Ang Tian,Chun-Jie Xu,Kai-Xia Zhou,Zhi-Gang Zhang,Jian-Ren Gu,Xue-Li Zhang,Ya-Hui Wang

Molecular medicine reports 18:1710-1717 PubMed29845198

2018

Hypoxia induces lactate secretion and glycolytic efflux by downregulating mitochondrial pyruvate carrier levels in human umbilical vein endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Wang,Qingjie Wang,Gaoliang Yan,Yong Qiao,Boqian Zhu,Bo Liu,Chengchun Tang

Oncology letters 15:9110-9116 PubMed29805642

2018

Function of mitochondrial pyruvate carriers in hepatocellular carcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangming Ma,Yunlong Cui,Hongyuan Zhou,Qiang Li

Nature communications 9:1208 PubMed29572438

2018

Interruption of lactate uptake by inhibiting mitochondrial pyruvate transport unravels direct antitumor and radiosensitizing effects.

Applications

Unspecified application

Species

Unspecified reactive species

Cyril Corbet,Estelle Bastien,Nihed Draoui,Bastien Doix,Lionel Mignion,Bénédicte F Jordan,Arnaud Marchand,Jean-Christophe Vanherck,Patrick Chaltin,Olivier Schakman,Holger M Becker,Olivier Riant,Olivier Feron

PloS one 10:e0144273 PubMed26642043

2015

Defective Expression of the Mitochondrial-tRNA Modifying Enzyme GTPBP3 Triggers AMPK-Mediated Adaptive Responses Involving Complex I Assembly Factors, Uncoupling Protein 2, and the Mitochondrial Pyruvate Carrier.

Applications

WB

Species

Human

Ana Martínez-Zamora,Salvador Meseguer,Juan M Esteve,Magda Villarroya,Carmen Aguado,J Antonio Enríquez,Erwin Knecht,M-Eugenia Armengod

PloS one 8:e62012 PubMed23650507

2013

Novel insulin sensitizer modulates nutrient sensing pathways and maintains β-cell phenotype in human islets.

Applications

ICC/IF

Species

Human

Nidhi Rohatgi,Haytham Aly,Connie A Marshall,William G McDonald,Rolf F Kletzien,Jerry R Colca,Michael L McDaniel
View all publications
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