Anti-MPC1 antibody [EPR29172-82]

• WB

Supplier Data

Western blot - Anti-MPC1 antibody [EPR29172-82] (AB324682)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : lung, spleen, pancreas (PMID : 27176894)

The identity of the higher MW bands above 75 kDa are unknow.

Lanes 1-4 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000, lanes 5-7 are incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MPC1 antibody [EPR29172-82] (ab324682) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate  at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Human liver tissue lysate at 20 µg

Lane 6:

Human lung tissue lysate at 20 µg

Lane 7:

Human pancreas tissue lysate at 20 µg

Secondary

Lanes 1 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 5 - 7:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 12 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-MPC1 antibody [EPR29172-82] (AB324682)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW bands above 75 kDa are unknow.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MPC1 antibody [EPR29172-82] (ab324682) at 1/1000 dilution

Lane 1:

293T mitochondrial fraction at 20 µg

Lane 2:

293T non-mitochondrial fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 12 kDa,16 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-MPC1 antibody [EPR29172-82] (AB324682)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW bands above 75 kDa are unknow.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MPC1 antibody [EPR29172-82] (ab324682) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate  at 20 µg

Lane 2:

HEK-293 transfected with siRNA specifically targeting MPC1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 12 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-MPC1 antibody [EPR29172-82] (AB324682)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW bands above 75 kDa are unknow.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MPC1 antibody [EPR29172-82] (ab324682) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 12 kDa,36 kDa

true

Exposure time: 180s