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AB196553

Anti-MPG/AAG antibody

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(3 Publications)

Rabbit Polyclonal MPG/AAG antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MPG.

View Alternative Names

AAG, ANPG, MID1, MPG, DNA-3-methyladenine glycosylase, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, ADPG, N-methylpurine-DNA glycosylase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody (AB196553)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody (AB196553)

The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using ab196553 at dilution 1/40, the image on the right is treated with fusion protein.

Western blot - Anti-MPG/AAG antibody (AB196553)
  • WB

Supplier Data

Western blot - Anti-MPG/AAG antibody (AB196553)

All lanes:

Western blot - Anti-MPG/AAG antibody (ab196553) at 1/500 dilution

Lane 1:

MCF7 extract

Lane 2:

K562 extract

Lane 3:

U937 extract

Lane 4:

Mouse liver extract

Predicted band size: 33 kDa

false

Western blot - Anti-MPG/AAG antibody (AB196553)
  • WB

Supplier Data

Western blot - Anti-MPG/AAG antibody (AB196553)

All lanes:

Western blot - Anti-MPG/AAG antibody (ab196553) at 1/950 dilution

Lane 1:

Lovo cell lysate

Lane 2:

PC3 cell lysate

Secondary

All lanes:

Goat anti rabbit IgG at 1/8000 dilution

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MPG.

P29372

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Alternative Products

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MPG also known as AAG or 3-methyladenine DNA glycosylase is a DNA repair protein with a molecular mass of approximately 33 kDa. It predominately acts to recognize and excise damaged bases from DNA playing a vital role in base excision repair (BER) mechanism. MPG/AAG is found in several tissues with high expression observed in liver and brain suggesting its importance in maintaining genomic stability in these areas.
Biological function summary

The MPG/AAG protein serves an essential function in cellular defense against genotoxic stress. It initiates the repair process by removing alkylated adenine and other modified bases from DNA. Although not directly identified as a part of any large protein complex MPG/AAG collaborates with other enzymatic components of BER such as DNA polymerase and ligase enzymes to facilitate complete and accurate DNA repair.

Pathways

MPG/AAG engages largely in the base excision repair pathway to correct single-base damage not addressed by nucleotide excision repair. Additionally its function ties into the cellular response to DNA alkylation a critical pathway concerning the maintenance of genomic integrity. MPG/AAG is often associated with DNA polymerase beta as a partner in extending and sealing the repaired strand ensuring the completion of the BER pathway.

The activity of MPG/AAG relates closely to cancer and neurodegenerative diseases. Alterations in MPG/AAG function have been linked to increased susceptibility to alkylation-induced tumorigenesis. Additionally impaired function can contribute to neuronal damage associating it with proteins involved in neurodegenerative processes such as Tau. These connections highlight the importance of MPG/AAG in both the initiation and progression of specific human diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
See full target information MPG
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3673 PubMed37339981

2023

SLC7A11 expression level dictates differential responses to oxidative stress in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuelong Yan,Hongqi Teng,Qinglei Hang,Lavanya Kondiparthi,Guang Lei,Amber Horbath,Xiaoguang Liu,Chao Mao,Shiqi Wu,Li Zhuang,M James You,Masha V Poyurovsky,Li Ma,Kellen Olszewski,Boyi Gan

Nature 606:930-936 PubMed35477155

2022

Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Shangming Tang,Ema Stokasimov,Yuxiang Cui,David Pellman

Environmental toxicology 36:850-860 PubMed33378118

2020

The role of H3K9me2-regulated base excision repair genes in the repair of DNA damage induced by arsenic in HaCaT cells and the effects of Ginkgo biloba extract intervention.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejiao Ding,Anliu Zhang,Changzhe Li,Lu Ma,Shunfang Tang,Qi Wang,Guanghong Yang,Jun Li
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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