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Rabbit Recombinant Monoclonal MPG/AAG antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.


Images

Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092), expandable thumbnail
  • Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092), expandable thumbnail

Publications

  • Scientific reports 11:183932021
    Versatile cell-based assay for measuring DNA alkylation damage and its repair.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Yong Li,Peng Mao,Evelina Y Basenko,Zachary Lewis,Michael J Smerdon,Wioletta Czaja
    PubMed 34526526
  • Nucleic acids research 45:10564-105822017
    Replication stress-induced endogenous DNA damage drives cellular senescence induced by a sub-lethal oxidative stress.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Gireedhar Venkatachalam,Uttam Surana,Marie-Véronique Clément
    PubMed 28985345

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Rat
Predicted
Expected
Predicted

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/10000 - 1/50000

Notes

-

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Product

    3 products for Alternative Version

      Target data

      Function

      Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

      Alternative names

      DNA-3-methyladenine glycosylase, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, ADPG, N-methylpurine-DNA glycosylase, MPG, MID1, AAG, ANPG

      Recommended products

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      Rabbit Recombinant Monoclonal MPG/AAG antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

      Alternative names

      DNA-3-methyladenine glycosylase, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, ADPG, N-methylpurine-DNA glycosylase, MPG, MID1, AAG, ANPG

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR10959(B)

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      MPG also known as AAG or 3-methyladenine DNA glycosylase is a DNA repair protein with a molecular mass of approximately 33 kDa. It predominately acts to recognize and excise damaged bases from DNA playing a vital role in base excision repair (BER) mechanism. MPG/AAG is found in several tissues with high expression observed in liver and brain suggesting its importance in maintaining genomic stability in these areas.

      Biological function summary

      The MPG/AAG protein serves an essential function in cellular defense against genotoxic stress. It initiates the repair process by removing alkylated adenine and other modified bases from DNA. Although not directly identified as a part of any large protein complex MPG/AAG collaborates with other enzymatic components of BER such as DNA polymerase and ligase enzymes to facilitate complete and accurate DNA repair.

      Pathways

      MPG/AAG engages largely in the base excision repair pathway to correct single-base damage not addressed by nucleotide excision repair. Additionally its function ties into the cellular response to DNA alkylation a critical pathway concerning the maintenance of genomic integrity. MPG/AAG is often associated with DNA polymerase beta as a partner in extending and sealing the repaired strand ensuring the completion of the BER pathway.

      Associated diseases and disorders

      The activity of MPG/AAG relates closely to cancer and neurodegenerative diseases. Alterations in MPG/AAG function have been linked to increased susceptibility to alkylation-induced tumorigenesis. Additionally impaired function can contribute to neuronal damage associating it with proteins involved in neurodegenerative processes such as Tau. These connections highlight the importance of MPG/AAG in both the initiation and progression of specific human diseases.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      9 product images

      • Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092), expandable thumbnail

        Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)

        Lane 1: Wild-type HAP1 cell lysate (20 μg)
        Lane 2: MPG/AAG knockout HAP1 cell lysate (20 μg)
        Lane 3: HeLa cell lysate (20 μg)
        Lane 4: HEK293 cell lysate (20 μg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab155092 observed at 35 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245.

        ab155092 was shown to specifically react with MPG/AAG when MPG/AAG knockout samples were used. Wild-type and MPG/AAG knockout samples were subjected to SDS-PAGE. ab155092 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/10,000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)

        Predicted band size: 33 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)

        Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155092 showing +ve staining.


        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092), expandable thumbnail

        Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)

        All lanes: Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092) at 1/10000 dilution

        Lane 1: HeLa cell lysate at 10 µg

        Lane 2: 293T cell lysate at 10 µg

        Lane 3: Jurkat cell lysate at 10 µg

        Secondary

        All lanes: Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 33 kDa

      • Flow Cytometry (Intracellular) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)

        Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MPG/AAG with unpurified ab155092 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)

        Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MPG/AAG with ab155092 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)

        Immunohistochemical analysis of paraffin-embedded Human tesis tissue labeling MPG/AAG with ab155092 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)

        Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155092 showing +ve staining.


        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)

        Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155092 showing +ve staining.


        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)

        Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155092 showing +ve staining.


        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

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