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AB155092

Anti-MPG/AAG antibody [EPR10959(B)]

5

(7 Reviews)

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(5 Publications)

Rabbit Recombinant Monoclonal MPG/AAG antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

AAG, ANPG, MID1, MPG, DNA-3-methyladenine glycosylase, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, ADPG, N-methylpurine-DNA glycosylase

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)
  • IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)

Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155092 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155092 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MPG/AAG with ab155092 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)

Immunohistochemical analysis of paraffin-embedded Human tesis tissue labeling MPG/AAG with ab155092 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MPG/AAG with unpurified ab155092 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)

Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155092 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155092 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)
  • WB

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Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)

All lanes:

Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa

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Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)
  • WB

Lab

Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (AB155092)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : MPG/AAG knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HEK293 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab155092 observed at 35 kDa. Red - loading control, ab8245.

ab155092 was shown to specifically react with MPG/AAG when MPG/AAG knockout samples were used. Wild-type and MPG/AAG knockout samples were subjected to SDS-PAGE. ab155092 and ab8245 (loading control to GAPDH) were diluted at 1/10,000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)

Predicted band size: 33 kDa

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  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-MPG/AAG antibody [EPR10959(B)]

  • 578 PE

    PE Anti-MPG antibody [EPR10959(B)]

  • 660 APC

    APC Anti-MPG antibody [EPR10959(B)]

  • HRP

    HRP Anti-MPG antibody [EPR10959(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-MPG antibody [EPR10959(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-MPG antibody [EPR10959(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-MPG antibody [EPR10959(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-MPG antibody [EPR10959(B)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-MPG/AAG antibody [EPR10959(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10959(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MPG also known as AAG or 3-methyladenine DNA glycosylase is a DNA repair protein with a molecular mass of approximately 33 kDa. It predominately acts to recognize and excise damaged bases from DNA playing a vital role in base excision repair (BER) mechanism. MPG/AAG is found in several tissues with high expression observed in liver and brain suggesting its importance in maintaining genomic stability in these areas.
Biological function summary

The MPG/AAG protein serves an essential function in cellular defense against genotoxic stress. It initiates the repair process by removing alkylated adenine and other modified bases from DNA. Although not directly identified as a part of any large protein complex MPG/AAG collaborates with other enzymatic components of BER such as DNA polymerase and ligase enzymes to facilitate complete and accurate DNA repair.

Pathways

MPG/AAG engages largely in the base excision repair pathway to correct single-base damage not addressed by nucleotide excision repair. Additionally its function ties into the cellular response to DNA alkylation a critical pathway concerning the maintenance of genomic integrity. MPG/AAG is often associated with DNA polymerase beta as a partner in extending and sealing the repaired strand ensuring the completion of the BER pathway.

The activity of MPG/AAG relates closely to cancer and neurodegenerative diseases. Alterations in MPG/AAG function have been linked to increased susceptibility to alkylation-induced tumorigenesis. Additionally impaired function can contribute to neuronal damage associating it with proteins involved in neurodegenerative processes such as Tau. These connections highlight the importance of MPG/AAG in both the initiation and progression of specific human diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
See full target information MPG
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e14599 PubMed38332576

2024

Transformed astrocytes confer temozolomide resistance on glioblastoma via delivering ALKBH7 to enhance APNG expression after educating by glioblastoma stem cells-derived exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Xinglei Liu,Liang Liu,Anyi Wu,Shilu Huang,Zhipeng Xu,Xiaopei Zhang,Zengyang Li,Haoran Li,Jun Dong

Scientific reports 11:18393 PubMed34526526

2021

Versatile cell-based assay for measuring DNA alkylation damage and its repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Li,Peng Mao,Evelina Y Basenko,Zachary Lewis,Michael J Smerdon,Wioletta Czaja

Nucleic acids research 45:10564-10582 PubMed28985345

2017

Replication stress-induced endogenous DNA damage drives cellular senescence induced by a sub-lethal oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Gireedhar Venkatachalam,Uttam Surana,Marie-Véronique Clément

Proceedings of the National Academy of Sciences of 112:3997-4002 PubMed25775545

2015

ATM prevents DSB formation by coordinating SSB repair and cell cycle progression.

Applications

WB

Species

Human

Svetlana V Khoronenkova,Grigory L Dianov

The Journal of cell biology 206:29-43 PubMed24982429

2014

DNA breaks and chromosomal aberrations arise when replication meets base excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Ensminger,Lucie Iloff,Christian Ebel,Teodora Nikolova,Bernd Kaina,Markus Lӧbrich
View all publications
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