Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (AB249186)

This data was developed using ab155092, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155092 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (AB249186)

This data was developed using ab155092, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155092 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (AB249186)

This data was developed using ab155092, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MPG/AAG with ab155092 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (AB249186)

This data was developed using ab155092, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tesis tissue labeling MPG/AAG with ab155092 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (AB249186)

This data was developed using ab155092, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MPG/AAG with unpurified ab155092 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (AB249186)

This data was developed using ab155092, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155092 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (AB249186)

This data was developed using ab155092, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155092 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (AB249186)

This data was developed using ab155092, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (<a href='/en-us/products/primary-antibodies/mpg-aag-antibody-epr10959b-ab155092'>ab155092</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa

false

• WB

Lab

Western blot - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (AB249186)

This data was developed using ab155092, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : MPG/AAG knockout HAP1 cell lysate (20 μg)

Lane 3 : HeLa cell lysate (20 μg)

Lane 4 : HEK293 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab155092 observed at 35 kDa. Red - loading control, ab8245.

ab155092 was shown to specifically react with MPG/AAG when MPG/AAG knockout samples were used. Wild-type and MPG/AAG knockout samples were subjected to SDS-PAGE. ab155092 and ab8245 (loading control to GAPDH) were diluted at 1/10,000 and 1/10,000 dilution respectively and incubated overnight at 4°C. Blots were developed with IRDye^® 800CW Goat anti-Rabbit IgG (H + L) and IRDye^® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (<a href='/en-us/products/primary-antibodies/mpg-aag-antibody-epr10959b-ab155092'>ab155092</a>)

Predicted band size: 33 kDa

false