Skip to main content

Rabbit Recombinant Monoclonal MPP1 antibody. Suitable for WB and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-MPP1 antibody [EPR5864] (AB133500), expandable thumbnail
  • Western blot - Anti-MPP1 antibody [EPR5864] (AB133500), expandable thumbnail
  • OI-RD Scanning - Anti-MPP1 antibody [EPR5864] (AB133500), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity (By similarity).

Alternative names

DXS552E, EMP55, MPP1, 55 kDa erythrocyte membrane protein, p55

Recommended products

Rabbit Recombinant Monoclonal MPP1 antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5864
Purity
Tissue culture supernatant
Dissociation constant
2.52 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The MPP1 protein also known as Membrane Palmitoylated Protein 1 acts as a part of the membrane-associated guanylate kinase (MAGUK) family. This protein has a molecular mass of around 55 kDa. MPP1 expresses mainly in erythrocytes the cells that carry oxygen in the blood. Functionally it plays a role in maintaining cell shape and stability by contributing to the cytoskeletal structure. It localizes at the plasma membrane and interacts with other proteins to fulfill its functions.

Biological function summary

In various cell types MPP1 supports cell adhesion and communication. As a complex-forming protein MPP1 interacts with spectrin and actin contributing to the structural organization of cell membranes. MPP1 regulates interactions within the cellular environment influencing cellular signaling and mechanical integrity. By establishing these connections it helps maintain cell shape and facilitate cell signaling processes.

Pathways

Several interactions actively involve MPP1. Within the pathways regulating cell shape MPP1 contributes to the RhoA signaling pathway important for cytoskeletal organization. Furthermore MPP1 closely works with proteins like Ezrin to support plasma membrane stability and intracellular communication. These interactions are significant for maintaining normal cellular junctions and morphology.

Associated diseases and disorders

MPP1 links to specific conditions with impacts on erythrocyte function. It has been implicated in hereditary spherocytosis a disorder affecting red blood cell stability and causing anemia. MPP1 also interacts with the protein Ankyrin an important component in maintaining erythrocyte membrane structure. These associations highlight the importance of MPP1 in blood-related diseases marking it as an important target for understanding membrane disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-MPP1 antibody [EPR5864] (ab133500), expandable thumbnail

    Western blot - Anti-MPP1 antibody [EPR5864] (ab133500)

    Lanes 1-4: Merged signal (red and green). Green - ab133500 observed at 52 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab133500 Anti-MPP1 antibody [EPR5864] was shown to specifically react with MPP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human MPP1 knockout HEK-293T cell line ab267248 (knockout cell lysate Human MPP1 knockout HEK-293T cell lysate ab258051) was used. Wild-type and MPP1 knockout samples were subjected to SDS-PAGE. ab133500 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MPP1 antibody [EPR5864] (ab133500) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: MPP1 knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human MPP1 knockout HEK-293T cell line (Human MPP1 knockout HEK-293T cell line ab267248)

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: K-562 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 52 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-MPP1 antibody [EPR5864] (ab133500), expandable thumbnail

    Western blot - Anti-MPP1 antibody [EPR5864] (ab133500)

    All lanes: Western blot - Anti-MPP1 antibody [EPR5864] (ab133500) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: 293T cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Lane 4: Human heart lysate at 10 µg

    Lane 5: K562 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 52 kDa

    Observed band size: 55 kDa

  • OI-RD Scanning - Anti-MPP1 antibody [EPR5864] (ab133500), expandable thumbnail

    OI-RD Scanning - Anti-MPP1 antibody [EPR5864] (ab133500)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com