AB108528

Anti-MPP1 antibody [EPR5865]

RabMAb
Recombinant
KO Validated
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal MPP1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DXS552E, EMP55, MPP1, 55 kDa erythrocyte membrane protein, p55

2 Images

Lab

Western blot - Anti-MPP1 antibody [EPR5865] (AB108528)

Lanes 1-4 : Merged signal (red and green). Green - ab108528 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.

ab108528 Anti-MPP1 antibody [EPR5865] was shown to specifically react with MPP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267248 (knockout cell lysate ab258051) was used. Wild-type and MPP1 knockout samples were subjected to SDS-PAGE. ab108528 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MPP1 antibody [EPR5865] (ab108528) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

MPP1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human MPP1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-mpp1-knockout-hek-293t-cell-line-ab267248'>ab267248</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

K-562 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Unknown

Western blot - Anti-MPP1 antibody [EPR5865] (AB108528)

All lanes:

Western blot - Anti-MPP1 antibody [EPR5865] (ab108528) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 10 kDa,21 kDa,52 kDa

Observed band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5865

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MPP1 protein also known as Membrane Palmitoylated Protein 1 acts as a part of the membrane-associated guanylate kinase (MAGUK) family. This protein has a molecular mass of around 55 kDa. MPP1 expresses mainly in erythrocytes the cells that carry oxygen in the blood. Functionally it plays a role in maintaining cell shape and stability by contributing to the cytoskeletal structure. It localizes at the plasma membrane and interacts with other proteins to fulfill its functions.

Biological function summary

In various cell types MPP1 supports cell adhesion and communication. As a complex-forming protein MPP1 interacts with spectrin and actin contributing to the structural organization of cell membranes. MPP1 regulates interactions within the cellular environment influencing cellular signaling and mechanical integrity. By establishing these connections it helps maintain cell shape and facilitate cell signaling processes.

Pathways

Several interactions actively involve MPP1. Within the pathways regulating cell shape MPP1 contributes to the RhoA signaling pathway important for cytoskeletal organization. Furthermore MPP1 closely works with proteins like Ezrin to support plasma membrane stability and intracellular communication. These interactions are significant for maintaining normal cellular junctions and morphology.

MPP1 links to specific conditions with impacts on erythrocyte function. It has been implicated in hereditary spherocytosis a disorder affecting red blood cell stability and causing anemia. MPP1 also interacts with the protein Ankyrin an important component in maintaining erythrocyte membrane structure. These associations highlight the importance of MPP1 in blood-related diseases marking it as an important target for understanding membrane disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity (By similarity).

See full target information MPP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 8:1547 PubMed29146910

2017

An unexpected protein interaction promotes drug resistance in leukemia.

Applications

Species

Human

Aaron Pitre,Yubin Ge,Wenwei Lin,Yao Wang,Yu Fukuda,Jamshid Temirov,Aaron H Phillips,Jennifer L Peters,Yiping Fan,Jing Ma,Amanda Nourse,Chandrima Sinha,Hai Lin,Richard Kriwacki,James R Downing,Tanja A Gruber,Victoria E Centonze,Anjaparavanda P Naren,Taosheng Chen,John D Schuetz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MPP1 antibody [EPR5865]

Western blot - Anti-MPP1 antibody [EPR5865] (AB108528)

Western blot - Anti-MPP1 antibody [EPR5865] (AB108528)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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