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AB204328

Anti-MPP10 antibody

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(1 Publication)

Rabbit Polyclonal MPP10 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MPHOSPH10 aa 450-550.

View Alternative Names

MPP10, MPHOSPH10, U3 small nucleolar ribonucleoprotein protein MPP10, M phase phosphoprotein 10

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP10 antibody (AB204328)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP10 antibody (AB204328)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling MPP10 with ab204328 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP10 antibody (AB204328)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP10 antibody (AB204328)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling MPP10 with ab204328 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP10 antibody (AB204328)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP10 antibody (AB204328)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling MPP10 with ab204328 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP10 antibody (AB204328)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP10 antibody (AB204328)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling MPP10 with ab204328 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry - Anti-MPP10 antibody (AB204328)
  • ICC

Supplier Data

Immunocytochemistry - Anti-MPP10 antibody (AB204328)

Immunofluorescent analysis of U2 OS cells (PFA-fixed/Triton X-100 permeabilized) labeling MPP10 with ab204328 at 4 μg/ml (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MPHOSPH10 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

O00566

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MPP10 also known as M-phase phosphoprotein 10 is a component of the nucleolar pre-40S ribosomal subunit. It has a molecular mass of approximately 85 kDa. MPP10 is expressed mainly in the nucleolus of eukaryotic cells where it plays a role in the assembly of ribosomal subunits. This protein acts as a scaffolding structure providing support and regulation necessary for proper ribosome biogenesis. Researchers have identified MPP10 with its involvement in nucleolar processes vital for cell growth and proliferation.
Biological function summary

The involvement of this protein in ribosomal RNA (rRNA) processing is significant. MPP10 is a part of the U3 small nucleolar ribonucleoprotein (snoRNP) complex essential for early cleavages of the pre-rRNA. This function helps initiate the maturation of the 18S rRNA which is a component of the small ribosomal subunit. The presence of MPP10 within this complex indicates its critical role in maintaining efficiency and fidelity during ribosome assembly. Its function impacts cellular protein synthesis influencing cellular growth and division.

Pathways

MPP10 plays an important role in ribosome biogenesis and related molecular pathways. It interacts within the nucleolar processing pathway of pre-18S ribosomal RNA. This pathway important for cell replication involves other proteins such as Fibrillarin and NOP56. MPP10's precise interaction with these proteins supports the maturation of pre-rRNA thereby ensuring the production of functional ribosomal subunits. Disruptions in these pathways can result in imbalances in cellular homeostasis affecting overall cellular function.

MPP10 has associations with pathological conditions related to ribosome dysfunction. Abnormal expression or mutations of MPP10 can contribute to disorders like cancer where inappropriate ribosome biogenesis accelerates tumor cell proliferation. Furthermore MPP10's association with other nucleolar proteins such as Dyskerin links it to ribosomopathies a group of disorders characterized by defective ribosome function. Understanding these connections helps researchers target ribosome-related pathways for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing (PubMed : 12655004). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information MPHOSPH10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Regenerative biomaterials 12:rbaf056 PubMed40741620

2025

Matrix stiffness boosts PDAC chemoresistance via SCD1-dependent lipid metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Zhang,Biwen Zhu,Jiashuai Yan,Xi Chen,Di Wu,Zhen Wang,Xiaoqi Guan,Yan Huang,Yahong Zhao,Yumin Yang,Yibing Guo
View all publications
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Product promise

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