Rabbit Polyclonal MPP10 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MPHOSPH10 aa 450-550.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MPHOSPH10 aa 450-550. The exact immunogen used to generate this antibody is proprietary information. Database link O00566
Reactivity data
Select an application| IHC-P | ICC | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing (PubMed:12655004). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Alternative names
MPP10, MPHOSPH10, U3 small nucleolar ribonucleoprotein protein MPP10, M phase phosphoprotein 10
Recommended products
Rabbit Polyclonal MPP10 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MPHOSPH10 aa 450-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MPHOSPH10 aa 450-550. The exact immunogen used to generate this antibody is proprietary information. Database link O00566
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MPP10 also known as M-phase phosphoprotein 10 is a component of the nucleolar pre-40S ribosomal subunit. It has a molecular mass of approximately 85 kDa. MPP10 is expressed mainly in the nucleolus of eukaryotic cells where it plays a role in the assembly of ribosomal subunits. This protein acts as a scaffolding structure providing support and regulation necessary for proper ribosome biogenesis. Researchers have identified MPP10 with its involvement in nucleolar processes vital for cell growth and proliferation.
- Biological function summary
The involvement of this protein in ribosomal RNA (rRNA) processing is significant. MPP10 is a part of the U3 small nucleolar ribonucleoprotein (snoRNP) complex essential for early cleavages of the pre-rRNA. This function helps initiate the maturation of the 18S rRNA which is a component of the small ribosomal subunit. The presence of MPP10 within this complex indicates its critical role in maintaining efficiency and fidelity during ribosome assembly. Its function impacts cellular protein synthesis influencing cellular growth and division.
- Pathways
MPP10 plays an important role in ribosome biogenesis and related molecular pathways. It interacts within the nucleolar processing pathway of pre-18S ribosomal RNA. This pathway important for cell replication involves other proteins such as Fibrillarin and NOP56. MPP10's precise interaction with these proteins supports the maturation of pre-rRNA thereby ensuring the production of functional ribosomal subunits. Disruptions in these pathways can result in imbalances in cellular homeostasis affecting overall cellular function.
- Associated diseases and disorders
MPP10 has associations with pathological conditions related to ribosome dysfunction. Abnormal expression or mutations of MPP10 can contribute to disorders like cancer where inappropriate ribosome biogenesis accelerates tumor cell proliferation. Furthermore MPP10's association with other nucleolar proteins such as Dyskerin links it to ribosomopathies a group of disorders characterized by defective ribosome function. Understanding these connections helps researchers target ribosome-related pathways for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunocytochemistry - Anti-MPP10 antibody (ab204328)
Immunofluorescent analysis of U2 OS cells (PFA-fixed/Triton X-100 permeabilized) labeling MPP10 with ab204328 at 4 μg/ml (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP10 antibody (ab204328)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling MPP10 with ab204328 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP10 antibody (ab204328)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling MPP10 with ab204328 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP10 antibody (ab204328)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling MPP10 with ab204328 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP10 antibody (ab204328)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling MPP10 with ab204328 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com