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Rabbit Polyclonal MPP8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MPHOSPH8 aa 400-550.

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Images

Western blot - Anti-MPP8 antibody (AB122611), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP8 antibody (AB122611), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MPP8 antibody (AB122611), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human MPHOSPH8 aa 400-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q99549

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000.00000 - 1/2500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/250.00000 - 1/500.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed:26022416, PubMed:28581500). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602).

Alternative names

Recommended products

Rabbit Polyclonal MPP8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MPHOSPH8 aa 400-550.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human MPHOSPH8 aa 400-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q99549
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle, Store undiluted

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MPP8 also known as M-phase phosphoprotein 8 or MPG functions mechanically as a chromodomain-containing protein involved in chromatin organization. It has a mass of approximately 92 kDa. MPP8 is expressed in several human tissues including the liver lung and brain. As an epigenetic regulator MPP8 plays a significant role in regulating gene expression through its interactions with chromatin.

Biological function summary

Within the cellular environment MPP8 participates in maintaining heterochromatin structure which is essential for proper chromosomal behavior. It is a component of the HUSH complex (human silencing hub) which is important for transcriptional repression. This protein complex involves other proteins like TASOR and PERIPHILIN collaborating to guide transcriptional silencing to specific genomic regions. MPP8's chromodomain allows it to recognize and bind to methylated histones further aiding its role in chromatin modification.

Pathways

MPP8 participates in epigenetic regulation and gene silencing pathways. It is notably involved in the silencing of retrotransposon elements through the HUSH complex. Proteins like TASOR enhance the silencing function of MPP8 in these pathways. MPP8 ensures proper chromatin state maintenance which influences downstream gene transcription processes. Proper functioning of these pathways is necessary for genomic stability.

Associated diseases and disorders

Deficiencies or dysfunctions of MPP8 have associations with cancer and neurodegenerative diseases. Abnormalities in MPP8 function may lead to improper gene silencing contributing to oncogenesis through deregulation of genes that control cell growth. In neurodegenerative contexts MPP8's role in maintaining chromatin structure may connect it to disease pathways involving chromatin remodeling proteins such as HP1 making it a focal point in studies on related disorders.

Product promise

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3 product images

  • Western blot - Anti-MPP8 antibody (ab122611), expandable thumbnail

    Western blot - Anti-MPP8 antibody (ab122611)

    All lanes: Western blot - Anti-MPP8 antibody (ab122611) at 1/250 dilution

    Lane 1: RT 4 lysate

    Lane 2: U 251 MG lysate

    Lane 3: Human Plasma lysate

    Lane 4: Human Liver lysate

    Lane 5: Human Tonsil lysate

    Developed using the ECL technique.

    Predicted band size: 97 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP8 antibody (ab122611), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP8 antibody (ab122611)

    ab122611, at 1/1000 dilution, staining MPP8 in Paraffin-embedded Human Colon tissue by Immunohistochemistry.

  • Immunocytochemistry/ Immunofluorescence - Anti-MPP8 antibody (ab122611), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MPP8 antibody (ab122611)

    ab122611, at 4μg/ml, staining MPP8 in PFA/Triton X-100 fixed/permealised U 251 MG cells by Immunofluorescence.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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