Rabbit Polyclonal MPP8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MPHOSPH8 aa 400-550.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/250.00000 - 1/500.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
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Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed:26022416, PubMed:28581500). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602).
MPP8, MPHOSPH8, M-phase phosphoprotein 8, Two hybrid-associated protein 3 with RanBPM, Twa3
Rabbit Polyclonal MPP8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MPHOSPH8 aa 400-550.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Avoid freeze / thaw cycle, Store undiluted
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This supplementary information is collated from multiple sources and compiled automatically.
MPP8 also known as M-phase phosphoprotein 8 or MPG functions mechanically as a chromodomain-containing protein involved in chromatin organization. It has a mass of approximately 92 kDa. MPP8 is expressed in several human tissues including the liver lung and brain. As an epigenetic regulator MPP8 plays a significant role in regulating gene expression through its interactions with chromatin.
Within the cellular environment MPP8 participates in maintaining heterochromatin structure which is essential for proper chromosomal behavior. It is a component of the HUSH complex (human silencing hub) which is important for transcriptional repression. This protein complex involves other proteins like TASOR and PERIPHILIN collaborating to guide transcriptional silencing to specific genomic regions. MPP8's chromodomain allows it to recognize and bind to methylated histones further aiding its role in chromatin modification.
MPP8 participates in epigenetic regulation and gene silencing pathways. It is notably involved in the silencing of retrotransposon elements through the HUSH complex. Proteins like TASOR enhance the silencing function of MPP8 in these pathways. MPP8 ensures proper chromatin state maintenance which influences downstream gene transcription processes. Proper functioning of these pathways is necessary for genomic stability.
Deficiencies or dysfunctions of MPP8 have associations with cancer and neurodegenerative diseases. Abnormalities in MPP8 function may lead to improper gene silencing contributing to oncogenesis through deregulation of genes that control cell growth. In neurodegenerative contexts MPP8's role in maintaining chromatin structure may connect it to disease pathways involving chromatin remodeling proteins such as HP1 making it a focal point in studies on related disorders.
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All lanes: Western blot - Anti-MPP8 antibody (ab122611) at 1/250 dilution
Lane 1: RT 4 lysate
Lane 2: U 251 MG lysate
Lane 3: Human Plasma lysate
Lane 4: Human Liver lysate
Lane 5: Human Tonsil lysate
Developed using the ECL technique.
Predicted band size: 97 kDa
ab122611, at 1/1000 dilution, staining MPP8 in Paraffin-embedded Human Colon tissue by Immunohistochemistry.
ab122611, at 4μg/ml, staining MPP8 in PFA/Triton X-100 fixed/permealised U 251 MG cells by Immunofluorescence.
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