Anti-MPP8 antibody [EPR28268-120] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-MPP8 antibody [EPR28268-120] - BSA and Azide free (AB324793)

This data was developed using ab322868, the same antibody clone in a different buffer formulation.

MPP8 was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab322868 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322868 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : ab322868 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab322868 in K-562 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-MPP8 antibody [EPR28268-120] (<a href='/en-us/products/primary-antibodies/mpp8-antibody-epr28268-120-ab322868'>ab322868</a>) at 1/30 dilution

All lanes:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-MPP8 antibody [EPR28268-120] - BSA and Azide free (AB324793)

This data was developed using ab322868, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands lower than 100 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MPP8 antibody [EPR28268-120] (<a href='/en-us/products/primary-antibodies/mpp8-antibody-epr28268-120-ab322868'>ab322868</a>) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

MCF7 transfected with siRNA specifically targeting MPP8 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 105 kDa,36 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-MPP8 antibody [EPR28268-120] - BSA and Azide free (AB324793)

This data was developed using ab322868, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands lower than 100 kDa are unknown.

Exposure time : Lanes 1-2 : 15 seconds; Lane 3 : 114 seconds.

All lanes:

Western blot - Anti-MPP8 antibody [EPR28268-120] (<a href='/en-us/products/primary-antibodies/mpp8-antibody-epr28268-120-ab322868'>ab322868</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 105 kDa

false