Rabbit Polyclonal MPRIP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human MPRIP aa 850 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MPRIP aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6WCQ1
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.
Alternative names
KIAA0864, MRIP, RHOIP3, MPRIP, Myosin phosphatase Rho-interacting protein, M-RIP, Rho-interacting protein 3, p116Rip, RIP3
Recommended products
Rabbit Polyclonal MPRIP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human MPRIP aa 850 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MPRIP aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6WCQ1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MPRIP also known as Myosin Phosphatase Rho Interacting Protein or p116Rip is an important player in the regulation of the actin cytoskeleton. This protein weighs approximately 116 kDa and is expressed in a variety of tissues including the brain heart and testis. It interacts with the regulatory subunit of myosin phosphatase influencing actin filament dynamics by modulating the interaction between actin and myosin.
- Biological function summary
MPRIP serves a critical role in the modulation of actin cytoskeleton rearrangement. It is part of a multi-protein complex that includes myosin phosphatase which is essential for dephosphorylation of myosin light chains. By binding directly to the myosin phosphatase complex MPRIP ensures proper regulation of actin-myosin interactions which are integral to cellular processes such as migration division and shape maintenance.
- Pathways
MPRIP plays an important role within the RhoA signaling pathway and the regulation of cytoskeletal rearrangements. It facilitates the interaction between RhoA and myosin phosphatase leading to the deactivation of contractility. Another important protein involved is ROCK as MPRIP counteracts ROCK-induced phosphorylation of myosin light chain therefore influencing cell contractility and motility.
- Associated diseases and disorders
MPRIP dysregulation has implications in cardiovascular diseases and cancer. Its connection with proteins like ROCK and RhoA highlights its role in these pathologies. In cardiovascular diseases improper MPRIP function can lead to aberrant vascular smooth muscle contraction while in cancer altered MPRIP activity may contribute to changes in cell migration and invasion affecting cancer progression and metastasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Immunocytochemistry/ Immunofluorescence - Anti-MPRIP antibody (ab251741)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MPRIP (green) using ab251741 at 4 µg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPRIP antibody (ab251741)
Immunohistochemical analysis of human Fallopian tube tissue labeling MPRIP with ab251741 at a 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPRIP antibody (ab251741)
Immunohistochemical analysis of human prostate tissue labeling MPRIP with ab251741 at a 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPRIP antibody (ab251741)
Immunohistochemical analysis of human liver tissue labeling MPRIP with ab251741 at a 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPRIP antibody (ab251741)
Immunohistochemical analysis of human cerebral cortex tissue labeling MPRIP with ab251741 at a 1/200 dilution.
Western blot - Anti-MPRIP antibody (ab251741)
All lanes: Western blot - Anti-MPRIP antibody (ab251741) at 0.4 µg/mL
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2: NBT-II (rat Wistar bladder tumor cell line) whole cell lysate
Predicted band size: 117 kDa
Western blot - Anti-MPRIP antibody (ab251741)
All lanes: Western blot - Anti-MPRIP antibody (ab251741) at 0.4 µg/mL
All lanes: PC-3 (human prostate adenocarcinoma cell line line) whole cell lysate
Predicted band size: 117 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com