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AB151541

Anti-MPZL antibody [EPR9229]

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(2 Publications)

Rabbit Recombinant Monoclonal MPZL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PZR, UNQ849/PRO1787, MPZL1, Myelin protein zero-like protein 1, Protein zero-related

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MPZL antibody [EPR9229] (AB151541)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MPZL antibody [EPR9229] (AB151541)

Immunofluorescent analysis of HepG2 cells labeling MPZL with ab151541 at 1/250 dilution.

Western blot - Anti-MPZL antibody [EPR9229] (AB151541)
  • WB

Lab

Western blot - Anti-MPZL antibody [EPR9229] (AB151541)

Lanes 1-4 : Merged signal (red and green). Green - ab151541 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa.

ab151541 Anti-MPZL antibody [EPR9229] was shown to specifically react with MPZL in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265332 (knockout cell lysate ab258053) was used. Wild-type and MPZL knockout samples were subjected to SDS-PAGE. ab151541 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MPZL antibody [EPR9229] (ab151541) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MPZL1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MPZL1 (MPZL) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-mpzl1-mpzl-knockout-hela-cell-line-ab265332'>ab265332</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

293T cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 37 kDa

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Western blot - Anti-MPZL antibody [EPR9229] (AB151541)
  • WB

Unknown

Western blot - Anti-MPZL antibody [EPR9229] (AB151541)

All lanes:

Western blot - Anti-MPZL antibody [EPR9229] (ab151541) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 29 kDa

false

  • Carrier free

    Anti-MPZL antibody [EPR9229] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9229

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein often referred to as MPZL or Myelin Protein Zero-like 1 (MPZL1) plays a significant role in cellular processes. It is a transmembrane glycoprotein with an approximated molecular mass of about 32.7 kDa. MPZL1 is expressed in various tissues with higher levels found in lung liver kidney and heart. Researchers also identify it under other names including Epithelial V-like Antigen (EVA) or IGSF8 and it typically contributes to cell adhesion and communication.
Biological function summary

MPZL1 supports critical functions like cell proliferation and differentiation through its interactions with other cellular components. It often associates with cytoplasmic signaling proteins suggesting its role in forming larger signaling complexes. MPZL1 engages in downstream signaling pathways when it binds to ligands influencing cellular dynamics and enabling proper tissue development and maintenance. Its interaction with other proteins furthers understanding of its involvement in cellular structural integrity.

Pathways

MPZL1 integrates into important cellular signaling cascades including the MAPK and PI3K-AKT pathways. Within these pathways MPZL1 modulates activity by contacting proteins like GRB2 and PIK3R1 influencing signals that are important for cellular growth and survival. Its activity in these pathways suggests an adaptive role in regulating cellular responses to external stimuli contributing to proper physiological function.

MPZL1 shows significant connection to cancer and fibrosis contexts. Researchers observe alterations in MPZL1 expression levels in certain cancers suggesting potential roles in tumor progression and metastasis. Additionally its link to fibrosis indicates a possible involvement in pathological tissue remodeling. Connections between MPZL1 PDGF receptors and fibrotic processes highlight the importance of understanding MPZL1 in disease mechanisms for potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor, which is involved in signal transduction processes. Recruits PTPN11/SHP-2 to the cell membrane and is a putative substrate of PTPN11/SHP-2. Is a major receptor for concanavalin-A (ConA) and is involved in cellular signaling induced by ConA, which probably includes Src family tyrosine-protein kinases. Isoform 3 seems to have a dominant negative role; it blocks tyrosine phosphorylation of MPZL1 induced by ConA. Isoform 1, but not isoform 2 and isoform 3, may be involved in regulation of integrin-mediated cell motility.
See full target information MPZL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 20:2725-2733 PubMed31322261

2019

MPZL1 is highly expressed in advanced gallbladder carcinoma and promotes the aggressive behavior of human gallbladder carcinoma GBC‑SD cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolei Liu,Jia Huang,Liguo Liu,Rong Liu

PloS one 10:e0123278 PubMed25859851

2015

Specific marker expression and cell state of Schwann cells during culture in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangyin Liu,Yu-Qing Jin,Lulu Chen,Yang Wang,Xiaonan Yang,Jia Cheng,Wei Wu,Zuoliang Qi,Zunli Shen
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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