Rabbit Recombinant Monoclonal MPZL antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Images
![Western blot - Anti-MPZL antibody [EPR9229] - BSA and Azide free (AB248994), expandable thumbnail](https://content.abcam.com/products/images/mpzl-antibody-epr9229-bsa-and-azide-free-ab248994--western-blot-img3516.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MPZL antibody [EPR9229] - BSA and Azide free (AB248994)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Cell surface receptor, which is involved in signal transduction processes. Recruits PTPN11/SHP-2 to the cell membrane and is a putative substrate of PTPN11/SHP-2. Is a major receptor for concanavalin-A (ConA) and is involved in cellular signaling induced by ConA, which probably includes Src family tyrosine-protein kinases. Isoform 3 seems to have a dominant negative role; it blocks tyrosine phosphorylation of MPZL1 induced by ConA. Isoform 1, but not isoform 2 and isoform 3, may be involved in regulation of integrin-mediated cell motility.
Alternative names
PZR, UNQ849/PRO1787, MPZL1, Myelin protein zero-like protein 1, Protein zero-related
Recommended products
Rabbit Recombinant Monoclonal MPZL antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9229
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248994 is the carrier-free version of Anti-MPZL antibody [EPR9229] ab151541.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein often referred to as MPZL or Myelin Protein Zero-like 1 (MPZL1) plays a significant role in cellular processes. It is a transmembrane glycoprotein with an approximated molecular mass of about 32.7 kDa. MPZL1 is expressed in various tissues with higher levels found in lung liver kidney and heart. Researchers also identify it under other names including Epithelial V-like Antigen (EVA) or IGSF8 and it typically contributes to cell adhesion and communication.
- Biological function summary
MPZL1 supports critical functions like cell proliferation and differentiation through its interactions with other cellular components. It often associates with cytoplasmic signaling proteins suggesting its role in forming larger signaling complexes. MPZL1 engages in downstream signaling pathways when it binds to ligands influencing cellular dynamics and enabling proper tissue development and maintenance. Its interaction with other proteins furthers understanding of its involvement in cellular structural integrity.
- Pathways
MPZL1 integrates into important cellular signaling cascades including the MAPK and PI3K-AKT pathways. Within these pathways MPZL1 modulates activity by contacting proteins like GRB2 and PIK3R1 influencing signals that are important for cellular growth and survival. Its activity in these pathways suggests an adaptive role in regulating cellular responses to external stimuli contributing to proper physiological function.
- Associated diseases and disorders
MPZL1 shows significant connection to cancer and fibrosis contexts. Researchers observe alterations in MPZL1 expression levels in certain cancers suggesting potential roles in tumor progression and metastasis. Additionally its link to fibrosis indicates a possible involvement in pathological tissue remodeling. Connections between MPZL1 PDGF receptors and fibrotic processes highlight the importance of understanding MPZL1 in disease mechanisms for potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-MPZL antibody [EPR9229] - BSA and Azide free (ab248994), expandable thumbnail](https://content.abcam.com/products/images/mpzl-antibody-epr9229-bsa-and-azide-free-ab248994--western-blot-img3516.jpg "Western blot - Anti-MPZL antibody [EPR9229] - BSA and Azide free (ab248994)")
Western blot - Anti-MPZL antibody [EPR9229] - BSA and Azide free (ab248994)
This data was developed using the same antibody clone in a different buffer formulation (Anti-MPZL antibody [EPR9229] ab151541).
Lanes 1-4: Merged signal (red and green). Green - Anti-MPZL antibody [EPR9229] ab151541 observed at 37 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.
Anti-MPZL antibody [EPR9229] ab151541 Anti-MPZL antibody [EPR9229] was shown to specifically react with MPZL in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human MPZL1 (MPZL) knockout HeLa cell line ab265332 (knockout cell lysate Human MPZL1 (MPZL) knockout HeLa cell lysate ab258053) was used. Wild-type and MPZL knockout samples were subjected to SDS-PAGE. Anti-MPZL antibody [EPR9229] ab151541 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-MPZL antibody [EPR9229] (Anti-MPZL antibody [EPR9229] ab151541) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: MPZL1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human MPZL1 (MPZL) knockout HeLa cell line (Human MPZL1 (MPZL) knockout HeLa cell line ab265332)
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: 293T cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 37 kDa
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