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AB230245

Anti-MRas antibody

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(1 Publication)

Rabbit Polyclonal MRas antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MRAS aa 50 to C-terminus.

View Alternative Names

RRAS3, MRAS, Ras-related protein M-Ras, Ras-related protein R-Ras3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRas antibody (AB230245)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRas antibody (AB230245)

Paraffin-embedded human testis tissue stained for MRas using ab230245 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-MRas antibody (AB230245)
  • WB

Supplier Data

Western blot - Anti-MRas antibody (AB230245)

All lanes:

Western blot - Anti-MRas antibody (ab230245) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MRAS aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O14807

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AB123181

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRas is a Ras-related GTP-binding protein often referred to as Ras-related protein M-Ras which belongs to the Ras superfamily of small GTPases. It has a molecular mass of about 24 kDa. MRas is expressed widely but shows higher levels in the brain and heart tissues. It functions by cycling between inactive GDP-bound and active GTP-bound states an action that modulates signal transduction pathways within cells. The process is imperative for its role in regulating cell dynamics.
Biological function summary

MRas plays a role in several cellular processes including cell growth differentiation and survival. It does not form part of larger complexes but interacts with various cytoplasmic and membrane proteins to propagate signals that control these cellular functions. Studies illustrate MRas's involvement in the regulation of cytoskeleton restructuring and cellular morphology further indicating its association with cell motility.

Pathways

MRas participates in the MAPK/ERK signaling pathway which is important for mediating cellular responses to growth signals. It associates with the Raf/MEK/ERK pathway components functioning upstream to influence these pathways. MRas's activity connects to other proteins like ERK1/2 allowing it to regulate gene expression involved in cell proliferation and survival.

MRas has been linked to cardiovascular diseases and certain types of cancer. Its role in cardiomyopathy stems from its involvement in cardiac cell signal transduction pathways. Additionally MRas activity can influence oncogenic pathways which relates to tumors driven by aberrant Ras signaling. The MRas interaction with Raf in particular is notable in its contribution to cancer pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival (PubMed : 16630891, PubMed : 28289718, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed : 16630891, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). The formation of the SMP complex only occurs when MRAS is GTP-bound (PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670, PubMed : 39809765). MRAS has low intrinsic GTPase activity and may require additional factors for activation (PubMed : 39809765). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed : 16630891, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670).
See full target information MRAS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:105745 PubMed36590171

2023

Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4.

Applications

Unspecified application

Species

Unspecified reactive species

Chenggang Zhang,Xianxiong Ma,Guanxin Wei,Xiuxian Zhu,Peng Hu,Xiang Chen,Dianshi Wang,Yuan Li,Tuo Ruan,Weikang Zhang,Kaixiong Tao,Chuanqing Wu
View all publications
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Product promise

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