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AB26303

Anti-MRas antibody

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(1 Publication)

Rabbit Polyclonal MRas antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MRAS aa 150-250.

View Alternative Names

RRAS3, MRAS, Ras-related protein M-Ras, Ras-related protein R-Ras3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MRas antibody (AB26303)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRas antibody (AB26303)

ICC/IF image of ab26303 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26303, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - Anti-MRas antibody (AB26303)
  • WB

Unknown

Western blot - Anti-MRas antibody (AB26303)

Observed band larger than predicted size, thought to be due to post-translational modifications.

All lanes:

Western blot - Anti-MRas antibody (ab26303)

Lane 1:

HEK 293T cell lysate

Lane 2:

MCF-7 cell lysate

Lane 3:

HeLa cell lysate

Secondary

All lanes:

anti-rabbit HRP conjugated

Predicted band size: 24 kDa

Observed band size: 32 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MRAS aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

O14807

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p>Block with 5% milk and dilute in TBST only.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRas is a Ras-related GTP-binding protein often referred to as Ras-related protein M-Ras which belongs to the Ras superfamily of small GTPases. It has a molecular mass of about 24 kDa. MRas is expressed widely but shows higher levels in the brain and heart tissues. It functions by cycling between inactive GDP-bound and active GTP-bound states an action that modulates signal transduction pathways within cells. The process is imperative for its role in regulating cell dynamics.
Biological function summary

MRas plays a role in several cellular processes including cell growth differentiation and survival. It does not form part of larger complexes but interacts with various cytoplasmic and membrane proteins to propagate signals that control these cellular functions. Studies illustrate MRas's involvement in the regulation of cytoskeleton restructuring and cellular morphology further indicating its association with cell motility.

Pathways

MRas participates in the MAPK/ERK signaling pathway which is important for mediating cellular responses to growth signals. It associates with the Raf/MEK/ERK pathway components functioning upstream to influence these pathways. MRas's activity connects to other proteins like ERK1/2 allowing it to regulate gene expression involved in cell proliferation and survival.

MRas has been linked to cardiovascular diseases and certain types of cancer. Its role in cardiomyopathy stems from its involvement in cardiac cell signal transduction pathways. Additionally MRas activity can influence oncogenic pathways which relates to tumors driven by aberrant Ras signaling. The MRas interaction with Raf in particular is notable in its contribution to cancer pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival (PubMed : 16630891, PubMed : 28289718, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed : 16630891, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). The formation of the SMP complex only occurs when MRAS is GTP-bound (PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670, PubMed : 39809765). MRAS has low intrinsic GTPase activity and may require additional factors for activation (PubMed : 39809765). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed : 16630891, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670).
See full target information MRAS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 67: PubMed40937572

2025

Progerin regulates actin cytoskeletal remodeling and inhibits EMT and metastasis in triple‑negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxian Huang,Weizhao Luo,Weixian Liu,Xinguang Liu,Weichun Chen
View all publications
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Product promise

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