Anti-MRas antibody [EPR12457] (ab176570) is a rabbit monoclonal antibody that is used to detect MRas in Western Blot, IP. Suitable for Human, Mouse samples.
- Specificity confirmed with MRas knockout cell line validation
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IP | Flow Cyt | WB | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Tested | Not recommended | Not recommended |
Mouse | Expected | Not recommended | Tested | Not recommended | Not recommended |
Rat | Predicted | Not recommended | Predicted | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
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Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival (PubMed:16630891, PubMed:28289718, PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed:16630891, PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). The formation of the SMP complex only occurs when MRAS is GTP-bound (PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed:16630891, PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670).
RRAS3, MRAS, Ras-related protein M-Ras, Ras-related protein R-Ras3
Anti-MRas antibody [EPR12457] (ab176570) is a rabbit monoclonal antibody that is used to detect MRas in Western Blot, IP. Suitable for Human, Mouse samples.
- Specificity confirmed with MRas knockout cell line validation
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
MRas is a Ras-related GTP-binding protein often referred to as Ras-related protein M-Ras which belongs to the Ras superfamily of small GTPases. It has a molecular mass of about 24 kDa. MRas is expressed widely but shows higher levels in the brain and heart tissues. It functions by cycling between inactive GDP-bound and active GTP-bound states an action that modulates signal transduction pathways within cells. The process is imperative for its role in regulating cell dynamics.
MRas plays a role in several cellular processes including cell growth differentiation and survival. It does not form part of larger complexes but interacts with various cytoplasmic and membrane proteins to propagate signals that control these cellular functions. Studies illustrate MRas's involvement in the regulation of cytoskeleton restructuring and cellular morphology further indicating its association with cell motility.
MRas participates in the MAPK/ERK signaling pathway which is important for mediating cellular responses to growth signals. It associates with the Raf/MEK/ERK pathway components functioning upstream to influence these pathways. MRas's activity connects to other proteins like ERK1/2 allowing it to regulate gene expression involved in cell proliferation and survival.
MRas has been linked to cardiovascular diseases and certain types of cancer. Its role in cardiomyopathy stems from its involvement in cardiac cell signal transduction pathways. Additionally MRas activity can influence oncogenic pathways which relates to tumors driven by aberrant Ras signaling. The MRas interaction with Raf in particular is notable in its contribution to cancer pathology.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Purified ab176570 at 1/20 dilution (1μg) immunoprecipitating MRas in Human heart lysate.
Lane 1 (input): Human heart lysate 10μg
Lane 2 (+): ab176570 + Human heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab176570 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 24 kDa
All lanes: Immunoprecipitation - Anti-MRas antibody [EPR12457] (ab176570)
Predicted band size: 24 kDa
All lanes: Western blot - Anti-MRas antibody [EPR12457] (ab176570) at 1/5000 dilution
Lane 1: Mouse brain lysate at 15 µg
Lane 2: Rat brain lysate at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 24 kDa
All lanes: Western blot - Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilution
All lanes: Human brain lysate
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 24 kDa
ab176570 Anti-MRas antibody [EPR12457] was shown to specifically react with MRas in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human MRAS knockout HeLa cell line ab265510 (knockout cell lysate Human MRAS knockout HeLa cell lysate ab257541) was used. Wild-type and MRas knockout samples were subjected to SDS-PAGE. ab176570 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: MRAS knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human MRAS knockout HeLa cell line (Human MRAS knockout HeLa cell line ab265510)
Lane 3: Human brain tissue lysate at 20 µg
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 26 kDa
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