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Anti-MRas antibody [EPR12457] (ab176570) is a rabbit monoclonal antibody detecting MRas in Western Blot, IP. Suitable for Human, Mouse.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency


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Images

Immunoprecipitation - Anti-MRas antibody [EPR12457] (AB176570), expandable thumbnail
  • Western blot - Anti-MRas antibody [EPR12457] (AB176570), expandable thumbnail
  • Western blot - Anti-MRas antibody [EPR12457] (AB176570), expandable thumbnail
  • Western blot - Anti-MRas antibody [EPR12457] (AB176570), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Not recommended
Mouse
Expected
Not recommended
Tested
Not recommended
Not recommended
Rat
Predicted
Not recommended
Predicted
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival (PubMed:16630891, PubMed:28289718, PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed:16630891, PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). The formation of the SMP complex only occurs when MRAS is GTP-bound (PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed:16630891, PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670).

Alternative names

RRAS3, MRAS, Ras-related protein M-Ras, Ras-related protein R-Ras3

Recommended products

Anti-MRas antibody [EPR12457] (ab176570) is a rabbit monoclonal antibody detecting MRas in Western Blot, IP. Suitable for Human, Mouse.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency


Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR12457
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-MRas antibody [EPR12457] (ab176570) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse samples.

What is the molecular weight of MRas?


Anti-MRas [EPR12457] (ab176570) specifically detects a band for MRas (UniProt: O14807) at a molecular weight of 24kDa.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-MRas antibody [EPR12457] (ab176570) has been confirmed by Western blot testing in MRAS Knockout HeLa cell line, Human MRAS knockout HeLa cell line ab265510.



Other related products


We have a range of other formats of antibody clone [EPR12457] also available for your convenience:
ab176570, Carrier free - Anti-MRas antibody [EPR12457] - BSA and Azide free ab240194



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MRas is a Ras-related GTP-binding protein often referred to as Ras-related protein M-Ras which belongs to the Ras superfamily of small GTPases. It has a molecular mass of about 24 kDa. MRas is expressed widely but shows higher levels in the brain and heart tissues. It functions by cycling between inactive GDP-bound and active GTP-bound states an action that modulates signal transduction pathways within cells. The process is imperative for its role in regulating cell dynamics.

Biological function summary

MRas plays a role in several cellular processes including cell growth differentiation and survival. It does not form part of larger complexes but interacts with various cytoplasmic and membrane proteins to propagate signals that control these cellular functions. Studies illustrate MRas's involvement in the regulation of cytoskeleton restructuring and cellular morphology further indicating its association with cell motility.

Pathways

MRas participates in the MAPK/ERK signaling pathway which is important for mediating cellular responses to growth signals. It associates with the Raf/MEK/ERK pathway components functioning upstream to influence these pathways. MRas's activity connects to other proteins like ERK1/2 allowing it to regulate gene expression involved in cell proliferation and survival.

Associated diseases and disorders

MRas has been linked to cardiovascular diseases and certain types of cancer. Its role in cardiomyopathy stems from its involvement in cardiac cell signal transduction pathways. Additionally MRas activity can influence oncogenic pathways which relates to tumors driven by aberrant Ras signaling. The MRas interaction with Raf in particular is notable in its contribution to cancer pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-MRas antibody [EPR12457] (ab176570), expandable thumbnail

    Immunoprecipitation - Anti-MRas antibody [EPR12457] (ab176570)

    Purified ab176570 at 1/20 dilution (1μg) immunoprecipitating MRas in Human heart lysate.
    Lane 1 (input): Human heart lysate 10μg
    Lane 2 (+): ab176570 + Human heart lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab176570 in Human heart lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 24 kDa

    All lanes: Immunoprecipitation - Anti-MRas antibody [EPR12457] (ab176570)

    Predicted band size: 24 kDa

  • Western blot - Anti-MRas antibody [EPR12457] (ab176570), expandable thumbnail

    Western blot - Anti-MRas antibody [EPR12457] (ab176570)

    All lanes: Western blot - Anti-MRas antibody [EPR12457] (ab176570) at 1/5000 dilution

    Lane 1: Mouse brain lysate at 15 µg

    Lane 2: Rat brain lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-MRas antibody [EPR12457] (ab176570), expandable thumbnail

    Western blot - Anti-MRas antibody [EPR12457] (ab176570)

    All lanes: Western blot - Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilution

    All lanes: Human brain lysate

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-MRas antibody [EPR12457] (ab176570), expandable thumbnail

    Western blot - Anti-MRas antibody [EPR12457] (ab176570)

    Lanes 1-3: Merged signal (red and green). Green - ab176570 observed at 26 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab176570 Anti-MRas antibody [EPR12457] was shown to specifically react with MRas in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human MRAS knockout HeLa cell line ab265510 (knockout cell lysate Human MRAS knockout HeLa cell lysate ab257541) was used. Wild-type and MRas knockout samples were subjected to SDS-PAGE. ab176570 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: MRAS knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human MRAS knockout HeLa cell line (Human MRAS knockout HeLa cell line ab265510)

    Lane 3: Human brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 24 kDa

    Observed band size: 26 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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