Anti-MRas antibody [EPR12457] (ab176570) is a rabbit monoclonal antibody that is used to detect MRas in Western Blot, IP. Suitable for Human, Mouse samples.
- Specificity confirmed with MRas knockout cell line validation
Images
![Immunoprecipitation - Anti-MRas antibody [EPR12457] (AB176570), expandable thumbnail](https://content.abcam.com/products/images/mras-antibody-epr12457-ab176570--immunoprecipitation-img3291.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MRas antibody [EPR12457] (AB176570)")
![Western blot - Anti-MRas antibody [EPR12457] (AB176570), expandable thumbnail](https://content.abcam.com/products/images/mras-antibody-epr12457-ab176570--western-blot-img51033.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MRas antibody [EPR12457] (AB176570)")
![Western blot - Anti-MRas antibody [EPR12457] (AB176570), expandable thumbnail](https://content.abcam.com/products/images/mras-antibody-epr12457-ab176570--western-blot-img8139.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MRas antibody [EPR12457] (AB176570)")
![Western blot - Anti-MRas antibody [EPR12457] (AB176570), expandable thumbnail](https://content.abcam.com/products/images/mras-antibody-epr12457-ab176570--western-blot-img5924.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MRas antibody [EPR12457] (AB176570)")
Publications
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- Cell death & disease 14:5562023LZTR1 deficiency exerts high metastatic potential by enhancing sensitivity to EMT induction and controlling KLHL12-mediated collagen secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaiki Abe,Shin-Ichiro Kanno,Tetsuya Niihori,Miho Terao,Shuji Takada,Yoko AokiPubMed 37626065
- The Journal of experimental medicine 220:2023Genome-wide CRISPR/Cas9 screens reveal shared and cell-specific mechanisms of resistance to SHP2 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wei,Mitchell J Geer,Xinyi Guo,Igor Dolgalev,Neville E Sanjana,Benjamin G NeelPubMed 36820830
- Cancer discovery 12:2434-24532022Impaired Proteolysis of Noncanonical RAS Proteins Drives Clonal Hematopoietic Transformation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSisi Chen,Rahul S Vedula,Antonio Cuevas-Navarro,Bin Lu,Simon J Hogg,Eric Wang,Salima Benbarche,Katherine Knorr,Won Jun Kim,Robert F Stanley,Hana Cho,Caroline Erickson,Michael Singer,Dan Cui,Steven Tittley,Benjamin H Durham,Tatiana S Pavletich,Elise Fiala,Michael F Walsh,Daichi Inoue,Sebastien Monette,Justin Taylor,Neal Rosen,Frank McCormick,R Coleman Lindsley,Pau Castel,Omar Abdel-WahabPubMed 35904492
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Expected | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Predicted | Not recommended | Predicted | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival (PubMed:16630891, PubMed:28289718, PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed:16630891, PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). The formation of the SMP complex only occurs when MRAS is GTP-bound (PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed:16630891, PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670).
Alternative names
RRAS3, MRAS, Ras-related protein M-Ras, Ras-related protein R-Ras3
Recommended products
Anti-MRas antibody [EPR12457] (ab176570) is a rabbit monoclonal antibody that is used to detect MRas in Western Blot, IP. Suitable for Human, Mouse samples.
- Specificity confirmed with MRas knockout cell line validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12457
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-MRas antibody [EPR12457] (ab176570) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse samples.
What is the molecular weight of MRas?
Anti-MRas [EPR12457] (ab176570) specifically detects a band for MRas (UniProt: O14807) at a molecular weight of 24kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-MRas antibody [EPR12457] (ab176570) has been confirmed by Western blot testing in MRAS Knockout HeLa cell line, Human MRAS knockout HeLa cell line ab265510.
Other related products
We have a range of other formats of antibody clone [EPR12457] also available for your convenience:
ab176570, Carrier free - Anti-MRas antibody [EPR12457] - BSA and Azide free ab240194
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MRas is a Ras-related GTP-binding protein often referred to as Ras-related protein M-Ras which belongs to the Ras superfamily of small GTPases. It has a molecular mass of about 24 kDa. MRas is expressed widely but shows higher levels in the brain and heart tissues. It functions by cycling between inactive GDP-bound and active GTP-bound states an action that modulates signal transduction pathways within cells. The process is imperative for its role in regulating cell dynamics.
- Biological function summary
MRas plays a role in several cellular processes including cell growth differentiation and survival. It does not form part of larger complexes but interacts with various cytoplasmic and membrane proteins to propagate signals that control these cellular functions. Studies illustrate MRas's involvement in the regulation of cytoskeleton restructuring and cellular morphology further indicating its association with cell motility.
- Pathways
MRas participates in the MAPK/ERK signaling pathway which is important for mediating cellular responses to growth signals. It associates with the Raf/MEK/ERK pathway components functioning upstream to influence these pathways. MRas's activity connects to other proteins like ERK1/2 allowing it to regulate gene expression involved in cell proliferation and survival.
- Associated diseases and disorders
MRas has been linked to cardiovascular diseases and certain types of cancer. Its role in cardiomyopathy stems from its involvement in cardiac cell signal transduction pathways. Additionally MRas activity can influence oncogenic pathways which relates to tumors driven by aberrant Ras signaling. The MRas interaction with Raf in particular is notable in its contribution to cancer pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-MRas antibody [EPR12457] (ab176570), expandable thumbnail](https://content.abcam.com/products/images/mras-antibody-epr12457-ab176570--immunoprecipitation-img3291.jpg "Immunoprecipitation - Anti-MRas antibody [EPR12457] (ab176570)")
Immunoprecipitation - Anti-MRas antibody [EPR12457] (ab176570)
Purified ab176570 at 1/20 dilution (1μg) immunoprecipitating MRas in Human heart lysate.
Lane 1 (input): Human heart lysate 10μg
Lane 2 (+): ab176570 + Human heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab176570 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 24 kDaAll lanes: Immunoprecipitation - Anti-MRas antibody [EPR12457] (ab176570)
Predicted band size: 24 kDa
![Western blot - Anti-MRas antibody [EPR12457] (ab176570), expandable thumbnail](https://content.abcam.com/products/images/mras-antibody-epr12457-ab176570--western-blot-img51033.jpg "Western blot - Anti-MRas antibody [EPR12457] (ab176570)")
Western blot - Anti-MRas antibody [EPR12457] (ab176570)
All lanes: Western blot - Anti-MRas antibody [EPR12457] (ab176570) at 1/5000 dilution
Lane 1: Mouse brain lysate at 15 µg
Lane 2: Rat brain lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 24 kDa
![Western blot - Anti-MRas antibody [EPR12457] (ab176570), expandable thumbnail](https://content.abcam.com/products/images/mras-antibody-epr12457-ab176570--western-blot-img8139.jpg "Western blot - Anti-MRas antibody [EPR12457] (ab176570)")
Western blot - Anti-MRas antibody [EPR12457] (ab176570)
All lanes: Western blot - Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilution
All lanes: Human brain lysate
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 24 kDa
![Western blot - Anti-MRas antibody [EPR12457] (ab176570), expandable thumbnail](https://content.abcam.com/products/images/mras-antibody-epr12457-ab176570--western-blot-img5924.jpg "Western blot - Anti-MRas antibody [EPR12457] (ab176570)")
Western blot - Anti-MRas antibody [EPR12457] (ab176570)
Lanes 1-3: Merged signal (red and green). Green - ab176570 observed at 26 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.ab176570 Anti-MRas antibody [EPR12457] was shown to specifically react with MRas in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human MRAS knockout HeLa cell line ab265510 (knockout cell lysate Human MRAS knockout HeLa cell lysate ab257541) was used. Wild-type and MRas knockout samples were subjected to SDS-PAGE. ab176570 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: MRAS knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human MRAS knockout HeLa cell line (Human MRAS knockout HeLa cell line ab265510)
Lane 3: Human brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 26 kDa
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