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Rabbit Recombinant Monoclonal MRC2/ENDO180 antibody. Suitable for ICC/IF, Flow Cyt, WB, IHC-P and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-MRC2/ENDO180 antibody [EPR29048-48] (AB317323), expandable thumbnail
  • Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (AB317323), expandable thumbnail
  • Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (AB317323), expandable thumbnail
  • Flow Cytometry - Anti-MRC2/ENDO180 antibody [EPR29048-48] (AB317323), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (AB317323), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWBIHC-PIP
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix cooperating with the matrix metalloproteinases (MMPs).

Alternative names

CD280, CLEC13E, ENDO180, KIAA0709, UPARAP, MRC2, C-type mannose receptor 2, C-type lectin domain family 13 member E, Endocytic receptor 180, Macrophage mannose receptor 2, Urokinase-type plasminogen activator receptor-associated protein, UPAR-associated protein, Urokinase receptor-associated protein

Recommended products

Rabbit Recombinant Monoclonal MRC2/ENDO180 antibody. Suitable for ICC/IF, Flow Cyt, WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29048-48
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MRC2 also known as the Endo180 protein is a type 1 transmembrane receptor with a structure that includes a fibronectin type II domain eight C-type lectin domains and a cysteine-rich domain. This protein has an approximate molecular mass of 180 kDa. MRC2 is widely expressed in various human tissues with noticeable expression in fibroblasts macrophages and certain endothelial cells. The protein plays a role in endocytosis involving the binding and internalization of collagen and other extracellular matrix components.

Biological function summary

MRC2 serves several significant roles including its involvement in tissue remodeling cellular migration and extracellular matrix turnover. The protein forms part of a larger complex involving other matrix-interacting proteins allowing for efficient capture and presentation of ligands. MRC2 contributes to the regulation of collagen homeostasis through interaction with collagenase and facilitates uptake and processing of collagen.

Pathways

MRC2 takes part in cellular processes connected to collagen metabolism and tissue remodeling. It is particularly involved in pathways such as the receptor-mediated endocytosis pathway and collagen degradation pathway. Interactions between MRC2 and proteins like uPAR (urokinase receptor) highlight its role in plasminogen activation which is essential for extracellular matrix breakdown and cell migration.

Associated diseases and disorders

MRC2 displays connection to tissue fibrosis and cancer metastasis. In fibrosis dysregulation of MRC2 can lead to excessive collagen deposition which contributes to disease progression. In cancer abnormal MRC2 activity associates with enhanced tumor cell invasion and metastasis. Additionally MRC2's interaction with uPAR suggests its involvement in tumor progression further emphasizing its potential as a therapeutic target for disease modulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MG 63 (human osteosarcoma fibroblast) cells labelling MRC2/ENDO180 with ab317323 at 1/100 (4.98 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in MG 63 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Negative control: MCF7 (PMID: 17974964).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323), expandable thumbnail

    Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)

    Low expression tissue: human brain (PMID: 8702911).

    The identity of the lower MW band at approximately 65 kDa (in lane 3) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane 1: 103 seconds; Lanes 2-3: 180 seconds.

    All lanes: Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323) at 1/1000 dilution

    Lane 1: Human liver cancer tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Human tonsil tissue lysate at 20 µg with NFDM/TBST

    Lane 3: Human brain tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 180 kDa, 36 kDa

  • Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323), expandable thumbnail

    Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)

    Low expression: MCF7, HepG2.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323) at 1/1000 dilution

    Lane 1: Saos-2 (human osteosarcoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 4: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 5: U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 6: A-172 (human brain glioblastoma cell ) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 180 kDa, 36 kDa

    Exposure time: 6s

  • Flow Cytometry - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323), expandable thumbnail

    Flow Cytometry - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)

    Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / MG-63(human osteosarcoma fibroblast, Right) cells labelling MRC2/ENDO180 with ab317323 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: MCF7

    Gated on viable cell.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling MRC2/ENDO180 with ab317323 at 1/500 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: No staining on human cerebrum

    (PMID: 8702911).

    The section was incubated with ab317323 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling MRC2/ENDO180 with ab317323 at 1/500 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on stromal cells of human breast carcinoma

    (PMID 26316068).

    The section was incubated with ab317323 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MRC2/ENDO180 with ab317323 at 1/500 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in macrophages of human tonsil

    (PMID: 8702911).

    The section was incubated with ab317323 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MRC2/ENDO180 with ab317323 at 1/500 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in endothelial cells of human kidney

    (PMID: 11156692).

    The section was incubated with ab317323 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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