Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

Rabbit Recombinant Monoclonal MRC2/ENDO180 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (AB317749), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	Flow Cyt	ICC/IF
Human	Tested	Tested	Tested	Not recommended	Not recommended
Mouse	Tested	Expected	Tested	Not recommended	Not recommended
Rat	Tested	Expected	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Target data

See full target information C-type mannose receptor 2

Function

May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix cooperating with the matrix metalloproteinases (MMPs).

Alternative names

CD280, CLEC13E, ENDO180, KIAA0709, UPARAP, MRC2, C-type mannose receptor 2, C-type lectin domain family 13 member E, Endocytic receptor 180, Macrophage mannose receptor 2, Urokinase-type plasminogen activator receptor-associated protein, UPAR-associated protein, Urokinase receptor-associated protein

Recommended products

Rabbit Recombinant Monoclonal MRC2/ENDO180 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR29048-78
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab317749 is the carrier-free version of Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MRC2 also known as the Endo180 protein is a type 1 transmembrane receptor with a structure that includes a fibronectin type II domain eight C-type lectin domains and a cysteine-rich domain. This protein has an approximate molecular mass of 180 kDa. MRC2 is widely expressed in various human tissues with noticeable expression in fibroblasts macrophages and certain endothelial cells. The protein plays a role in endocytosis involving the binding and internalization of collagen and other extracellular matrix components.

Biological function summary

MRC2 serves several significant roles including its involvement in tissue remodeling cellular migration and extracellular matrix turnover. The protein forms part of a larger complex involving other matrix-interacting proteins allowing for efficient capture and presentation of ligands. MRC2 contributes to the regulation of collagen homeostasis through interaction with collagenase and facilitates uptake and processing of collagen.

Pathways

MRC2 takes part in cellular processes connected to collagen metabolism and tissue remodeling. It is particularly involved in pathways such as the receptor-mediated endocytosis pathway and collagen degradation pathway. Interactions between MRC2 and proteins like uPAR (urokinase receptor) highlight its role in plasminogen activation which is essential for extracellular matrix breakdown and cell migration.

Associated diseases and disorders

MRC2 displays connection to tissue fibrosis and cancer metastasis. In fibrosis dysregulation of MRC2 can lead to excessive collagen deposition which contributes to disease progression. In cancer abnormal MRC2 activity associates with enhanced tumor cell invasion and metastasis. Additionally MRC2's interaction with uPAR suggests its involvement in tumor progression further emphasizing its potential as a therapeutic target for disease modulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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13 product images

Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
Low expression tissue: brain (PMID: 8702911).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-3: 180 seconds; Lane 4: 15 seconds; Lanes 5-7: 59 seconds.
All lanes: Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-78] (Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748) at 1/1000 dilution
Lane 1: Mouse breast tissue lysate at 20 µg with NFDM/TBST
Lane 2: Mouse brain tissue lysate at 20 µg with NFDM/TBST
Lane 3: Mouse spleen tissue lysate at 20 µg with NFDM/TBST
Lane 4: Mouse lung tissue lysate at 20 µg with NFDM/TBST
Lane 5: Rat kidney tissue lysate at 20 µg with NFDM/TBST
Lane 6: Rat spleen tissue lysate at 20 µg with NFDM/TBST
Lane 7: Rat brain tissue lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 180 kDa, 36 kDa
Immunoprecipitation - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
MRC2/ENDO180 was immunoprecipitated from 0.35 mg A-172 (human brain glioblastoma cell ) whole cell lysate with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: A-172 (human brain glioblastoma cell ) whole cell lysate

Lane 2: Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 IP in A-172 (human brain glioblastoma cell ) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 in A-172 whole cell lysate
All lanes: Immunoprecipitation - Anti-MRC2/ENDO180 antibody [EPR29048-78] (Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748) at 1/30 dilution
All lanes: A-172 (human brain glioblastoma cell) whole cell lysate with NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 32s
Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
Low expression: MCF7, HepG2.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-4: 26 seconds; Lanes 5-6: 8 seconds.
All lanes: Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-78] (Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748) at 1/1000 dilution
Lane 1: Saos-2 (human osteosarcoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg with NFDM/TBST
Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 4: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 5: U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 6: A-172 (human brain glioblastoma cell) whole cell lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 180 kDa, 36 kDa
Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
Low expression tissue: brain (PMID: 8702911).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-78] (Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748) at 1/1000 dilution
Lane 1: Human liver cancer tissue lysate at 20 µg with NFDM/TBST
Lane 2: Human tonsil tissue lysate at 20 µg with NFDM/TBST
Lane 3: Human breast tissue lysate at 20 µg with NFDM/TBST
Lane 4: Human kidney tissue lysate at 20 µg with NFDM/TBST
Lane 5: Human brain tissue lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 180 kDa, 36 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MRC2/ENDO180 with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: weak staining on endothelium cells of rat cerebrum.

The section was incubated with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling MRC2/ENDO180 with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: weak staining on endothelium cells of human cerebrum (PMID: 8702911).

The section was incubated with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MRC2/ENDO180 with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: weak staining on endothelium cells of mouse cerebrum.

The section was incubated with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse breast carcinoma tissue labeling MRC2/ENDO180 with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal cells of mouse breast carcinoma.

The section was incubated with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling MRC2/ENDO180 with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal cells of rat kidney.

The section was incubated with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MRC2/ENDO180 with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal cells of mouse kidney.

The section was incubated with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling MRC2/ENDO180 with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal cells of human breast carcinoma (PMID 26316068).

The section was incubated with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MRC2/ENDO180 with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil (PMID: 8702911).

The section was incubated with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)
This data was developed using Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MRC2/ENDO180 with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal cells of human kidney (PMID: 11156692).

The section was incubated with Anti-MRC2/ENDO180 antibody [EPR29048-78] ab317748 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins