Mouse Monoclonal MRE11 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 97 publications. Immunogen corresponding to Synthetic Peptide within Human MRE11 aa 150-600.
Images
![Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214), expandable thumbnail](https://content.abcam.com/products/images/mre11-antibody-12d7-bsa-and-azide-free-ab214--flow-cytometry-img78800.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214)")
![Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214), expandable thumbnail](https://content.abcam.com/products/images/mre11-antibody-12d7-bsa-and-azide-free-ab214--western-blot-img45432.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214)")
![Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214), expandable thumbnail](https://content.abcam.com/products/images/mre11-antibody-12d7-bsa-and-azide-free-ab214--immunocytochemistry-immunofluorescence-img119146.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214)")
![Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214), expandable thumbnail](https://content.abcam.com/products/images/mre11-antibody-12d7-bsa-and-azide-free-ab214--western-blot-img56743.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214)")
![Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214), expandable thumbnail](https://content.abcam.com/products/images/mre11-antibody-12d7-bsa-and-azide-free-ab214--immunocytochemistry-immunofluorescence-img94020.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214)")
Publications
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- Nature communications 14:78822023CaMKK2 and CHK1 phosphorylate human STN1 in response to replication stress to protect stalled forks from aberrant resection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRishi Kumar Jaiswal,Kai-Hang Lei,Megan Chastain,Yuan Wang,Olga Shiva,Shan Li,Zhongsheng You,Peter Chi,Weihang ChaiPubMed 38036565
- International journal of molecular sciences 24:2023Selective Killing of BRCA2-Deficient Ovarian Cancer Cells via MRE11 Blockade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdel Alblihy,Reem Ali,Mashael Algethami,Alison A Ritchie,Ahmed Shoqafi,Shatha Alqahtani,Katia A Mesquita,Michael S Toss,Paloma Ordóñez-Morán,Jennie N Jeyapalan,Lodewijk Dekker,Martina Salerno,Edgar Hartsuiker,Anna M Grabowska,Emad A Rakha,Nigel P Mongan,Srinivasan MadhusudanPubMed 37446144
- Nature communications 14:24282023Pathway choice in the alternative telomere lengthening in neoplasia is dictated by replication fork processing mediated by EXD2's nuclease activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRonan Broderick,Veronica Cherdyntseva,Jadwiga Nieminuszczy,Eleni Dragona,Maria Kyriakaki,Theodora Evmorfopoulou,Sarantis Gagos,Wojciech NiedzwiedzPubMed 37105990
- Cancers 15:2023Prognostic Significance of MRE11 Overexpression in Colorectal Cancer Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVincent Ho,Liping Chung,Kate Wilkinson,Vivienne Lea,Stephanie H Lim,Askar Abubakar,Weng Ng,Mark Lee,Tara L Roberts,Wei Chua,Cheok Soon LeePubMed 37173905
- Genes & development 37:119-1352023PLK1 regulates CtIP and DNA2 interplay in long-range DNA end resection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlaria Ceppi,Elda Cannavo,Hélène Bret,Rosa Camarillo,Francesca Vivalda,Roshan Singh Thakur,Amador Romero-Franco,Alessandro A Sartori,Pablo Huertas,Raphaël Guérois,Petr CejkaPubMed 36746606
- Cell death & disease 13:7092022DNA damage-induced paraspeckle formation enhances DNA repair and tumor radioresistance by recruiting ribosomal protein P0.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Long Wang,Wan-Wen Zhao,Shao-Mei Bai,Yan Ma,Xin-Ke Yin,Li-Li Feng,Guang-Dong Zeng,Fang Wang,Wei-Xing Feng,Jian Zheng,Ying-Nai Wang,Bing Zeng,Quentin Liu,Mien-Chie Hung,Xiang-Bo WanPubMed 35974014
- NPJ precision oncology 6:512022Targeting Mre11 overcomes platinum resistance and induces synthetic lethality in XRCC1 deficient epithelial ovarian cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdel Alblihy,Reem Ali,Mashael Algethami,Ahmed Shoqafi,Michael S Toss,Juliette Brownlie,Natalie J Tatum,Ian Hickson,Paloma Ordonez Moran,Anna Grabowska,Jennie N Jeyapalan,Nigel P Mongan,Emad A Rakha,Srinivasan MadhusudanPubMed 35853939
- Cell biology international 46:1625-16362022Comparison of DNA stability and its related genes of neurons derived from induced pluripotent stem cells and primary retinal neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Yu,Jin Qiu,Kaijing Li,Qian Luo,Xuan Liu,Ying Yang,Zihua Jiang,Shengyu He,Anqi He,Shuilian Chen,Xi Chen,Yan Li,Jian Ge,Jing Zhuang,Keming YuPubMed 35771585
- EMBO reports 23:e54483202253BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStavroula Tsaridou,Georgia Velimezi,Frances Willenbrock,Maria Chatzifrangkeskou,Waheba Elsayed,Andreas Panagopoulos,Dimitris Karamitros,Vassilis Gorgoulis,Zoi Lygerou,Vassilis Roukos,Eric O'Neill,Dafni Eleftheria PefaniPubMed 35758159
- Theranostics 12:4386-43982022Targeting type Iγ phosphatidylinositol phosphate kinase overcomes oxaliplatin resistance in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinhao Yu,Hao Wang,Wei Zhao,Xiaoxiao Ge,Wei Huang,Fengjuan Lin,Wenbo Tang,Ang Li,Sailiang Liu,Rong-Kun Li,Shu-Heng Jiang,Junli XuePubMed 35673560
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human MRE11 aa 150-600. The exact immunogen used to generate this antibody is proprietary information. Database link P49959
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For normal lymphoblastoid cell lines. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes For normal lymphoblastoid cell lines. |
Species Rat | Dilution info - | Notes For normal lymphoblastoid cell lines. |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes (see Robinson et al). |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes (see Robinson et al). |
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:11741547, PubMed:14657032, PubMed:22078559, PubMed:23080121, PubMed:24316220, PubMed:26240375, PubMed:27889449, PubMed:28867292, PubMed:29670289, PubMed:30464262, PubMed:30612738, PubMed:31353207, PubMed:37696958, PubMed:38128537, PubMed:9590181, PubMed:9651580, PubMed:9705271). The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases (PubMed:24316220, PubMed:28867292, PubMed:31353207, PubMed:38128537). The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage (PubMed:24316220, PubMed:27889449, PubMed:28867292, PubMed:36050397, PubMed:38128537). Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity (PubMed:11741547, PubMed:22078559, PubMed:24316220, PubMed:26240375, PubMed:27889449, PubMed:29670289, PubMed:31353207, PubMed:36563124, PubMed:9590181, PubMed:9651580, PubMed:9705271). After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with RBBP8/CtIP to initiate end resection (PubMed:27814491, PubMed:27889449, PubMed:30787182). MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR (PubMed:24316220). It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation to create entry sites for EXO1- and DNA2-mediated 5' to 3' long-range resection, which is required for single-strand invasion and recombination (PubMed:24316220, PubMed:28867292). RBBP8/CtIP specifically promotes the endonuclease activity of MRE11 to clear protein-DNA adducts and generate clean double-strand break ends (PubMed:27814491, PubMed:27889449, PubMed:30787182). The MRN complex is also required for DNA damage signaling via activation of the ATM and ATR kinases: the nuclease activity of MRE11 is not required to activate ATM and ATR (PubMed:14657032, PubMed:15064416, PubMed:15790808, PubMed:16622404). The MRN complex is also required for the processing of R-loops (PubMed:31537797). The MRN complex is involved in the activation of the cGAS-STING pathway induced by DNA damage during tumorigenesis: the MRN complex acts by displacing CGAS from nucleosome sequestration, thereby activating it (By similarity). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888). MRE11 contains two DNA-binding domains (DBDs), enabling it to bind both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA).
Alternative names
HNGS1, MRE11A, MRE11, Double-strand break repair protein MRE11, Meiotic recombination 11 homolog 1, Meiotic recombination 11 homolog A, MRE11 homolog 1, MRE11 homolog A
Recommended products
Mouse Monoclonal MRE11 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 97 publications. Immunogen corresponding to Synthetic Peptide within Human MRE11 aa 150-600.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human MRE11 aa 150-600. The exact immunogen used to generate this antibody is proprietary information. Database link P49959
- Carrier free
- Yes
- Clone number
- 12D7
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 10th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Mre11 protein also known as MRE11A serves as an integral part of the DNA repair machinery in cells. It forms a component of the MRE11/RAD50/NBS1 (MRN) complex which is essential for the detection and repair of DNA double-strand breaks (DSBs). The molecular weight of the Mre11 protein is approximately 81 kDa. It is broadly expressed in various human tissues highlighting its extensive role in maintaining genomic stability.
- Biological function summary
This protein acts in the repair of DSBs by initiating homologous recombination and non-homologous end joining pathways. Together with the RAD50 and NBS1 proteins Mre11 forms the MRN complex which processes DNA ends and signals to other repair mechanisms. Additionally Mre11's exonuclease and endonuclease activities are important for the resection of DNA at break sites facilitating subsequent repair synthesis.
- Pathways
Mre11 is instrumental in the DNA damage response and maintenance of genomic integrity. It operates within the ATM (ataxia-telangiectasia mutated) signaling pathway which activates upon DNA damage and regulates cell cycle checkpoints. Mre11 interacts with the ATM protein modifying the cellular response to DNA damage. The complex also collaborates closely with BRCA1 an important regulator of the repair process and associated with preventing breast cancer development.
- Associated diseases and disorders
Mutations or dysfunction in the MRE11A gene can be linked to several conditions including ataxia-telangiectasia-like disorder (ATLD) and Nijmegen breakage syndrome (NBS). These disorders result from impaired DNA repair mechanisms leading to increased sensitivity to radiation and predisposition to cancer. The NBS1 protein as part of the MRN complex works closely with Mre11 in these conditions. Both disorders highlight the critical role of Mre11 in safeguarding genomic stability and preventing disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
![Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214), expandable thumbnail](https://content.abcam.com/products/images/mre11-antibody-12d7-bsa-and-azide-free-ab214--flow-cytometry-img78800.jpg "Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)")
Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
![Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214), expandable thumbnail](https://content.abcam.com/products/images/mre11-antibody-12d7-bsa-and-azide-free-ab214--western-blot-img45432.jpg "Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)")
Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
7.5% SDS-PAGE
All lanes: Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214) at 1/1000 dilution
Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Lane 4: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: anti-mouse IgG HRP-conjugated antibody
Predicted band size: 80 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214), expandable thumbnail](https://content.abcam.com/products/images/mre11-antibody-12d7-bsa-and-azide-free-ab214--immunocytochemistry-immunofluorescence-img119146.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)")
Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
Immunocytochemical analysis of, 4% paraformaldehyde-fixed at RT for 15 min, HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mre-11 (green) with ab214 at 1/200 dilution. Blue: Hoechst 33342 staining. Scale bar= 10 μm.
![Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214), expandable thumbnail](https://content.abcam.com/products/images/mre11-antibody-12d7-bsa-and-azide-free-ab214--western-blot-img56743.png "Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)")
Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
This image was generated using the ascites version of the product.
7.5% SDS-PAGE
All lanes: Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214) at 1/1000 dilution
Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: Human Mre-11-transfected HEK-293T whole cell lysate at 30 µg
SecondaryAll lanes: anti-mouse IgG HRP-conjugated antibody
Predicted band size: 80 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214), expandable thumbnail](https://content.abcam.com/products/images/mre11-antibody-12d7-bsa-and-azide-free-ab214--immunocytochemistry-immunofluorescence-img94020.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)")
Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
(Panel D - negative control).This image was generated using the ascites version of the product.
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