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Mouse Monoclonal MRE11 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 97 publications. Immunogen corresponding to Synthetic Peptide within Human MRE11 aa 150-600.


Images

Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214), expandable thumbnail
  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214), expandable thumbnail
  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (AB214), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human MRE11 aa 150-600. The exact immunogen used to generate this antibody is proprietary information. Database link P49959

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IF
Human
Expected
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Expected
Expected

Species
Human
Dilution info
-
Notes

For normal lymphoblastoid cell lines.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

For normal lymphoblastoid cell lines.

Species
Rat
Dilution info
-
Notes

For normal lymphoblastoid cell lines.

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg for 106 Cells
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/3000.00000
Notes

(see Robinson et al).

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

(see Robinson et al).

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:11741547, PubMed:14657032, PubMed:22078559, PubMed:23080121, PubMed:24316220, PubMed:26240375, PubMed:27889449, PubMed:28867292, PubMed:29670289, PubMed:30464262, PubMed:30612738, PubMed:31353207, PubMed:37696958, PubMed:38128537, PubMed:9590181, PubMed:9651580, PubMed:9705271). The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases (PubMed:24316220, PubMed:28867292, PubMed:31353207, PubMed:38128537). The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage (PubMed:24316220, PubMed:27889449, PubMed:28867292, PubMed:36050397, PubMed:38128537). Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity (PubMed:11741547, PubMed:22078559, PubMed:24316220, PubMed:26240375, PubMed:27889449, PubMed:29670289, PubMed:31353207, PubMed:36563124, PubMed:9590181, PubMed:9651580, PubMed:9705271). After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with RBBP8/CtIP to initiate end resection (PubMed:27814491, PubMed:27889449, PubMed:30787182). MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR (PubMed:24316220). It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation to create entry sites for EXO1- and DNA2-mediated 5' to 3' long-range resection, which is required for single-strand invasion and recombination (PubMed:24316220, PubMed:28867292). RBBP8/CtIP specifically promotes the endonuclease activity of MRE11 to clear protein-DNA adducts and generate clean double-strand break ends (PubMed:27814491, PubMed:27889449, PubMed:30787182). The MRN complex is also required for DNA damage signaling via activation of the ATM and ATR kinases: the nuclease activity of MRE11 is not required to activate ATM and ATR (PubMed:14657032, PubMed:15064416, PubMed:15790808, PubMed:16622404). The MRN complex is also required for the processing of R-loops (PubMed:31537797). The MRN complex is involved in the activation of the cGAS-STING pathway induced by DNA damage during tumorigenesis: the MRN complex acts by displacing CGAS from nucleosome sequestration, thereby activating it (By similarity). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888). MRE11 contains two DNA-binding domains (DBDs), enabling it to bind both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA).

Alternative names

Recommended products

Mouse Monoclonal MRE11 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 97 publications. Immunogen corresponding to Synthetic Peptide within Human MRE11 aa 150-600.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human MRE11 aa 150-600. The exact immunogen used to generate this antibody is proprietary information. Database link P49959
Carrier free
Yes
Clone number
12D7
Purification technique
Affinity purification Protein G
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 10th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Mre11 protein also known as MRE11A serves as an integral part of the DNA repair machinery in cells. It forms a component of the MRE11/RAD50/NBS1 (MRN) complex which is essential for the detection and repair of DNA double-strand breaks (DSBs). The molecular weight of the Mre11 protein is approximately 81 kDa. It is broadly expressed in various human tissues highlighting its extensive role in maintaining genomic stability.

Biological function summary

This protein acts in the repair of DSBs by initiating homologous recombination and non-homologous end joining pathways. Together with the RAD50 and NBS1 proteins Mre11 forms the MRN complex which processes DNA ends and signals to other repair mechanisms. Additionally Mre11's exonuclease and endonuclease activities are important for the resection of DNA at break sites facilitating subsequent repair synthesis.

Pathways

Mre11 is instrumental in the DNA damage response and maintenance of genomic integrity. It operates within the ATM (ataxia-telangiectasia mutated) signaling pathway which activates upon DNA damage and regulates cell cycle checkpoints. Mre11 interacts with the ATM protein modifying the cellular response to DNA damage. The complex also collaborates closely with BRCA1 an important regulator of the repair process and associated with preventing breast cancer development.

Associated diseases and disorders

Mutations or dysfunction in the MRE11A gene can be linked to several conditions including ataxia-telangiectasia-like disorder (ATLD) and Nijmegen breakage syndrome (NBS). These disorders result from impaired DNA repair mechanisms leading to increased sensitivity to radiation and predisposition to cancer. The NBS1 protein as part of the MRN complex works closely with Mre11 in these conditions. Both disorders highlight the critical role of Mre11 in safeguarding genomic stability and preventing disease.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214), expandable thumbnail

    Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)

    Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214), expandable thumbnail

    Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)

    7.5% SDS-PAGE

    All lanes: Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214) at 1/1000 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Lane 4: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Secondary

    All lanes: anti-mouse IgG HRP-conjugated antibody

    Predicted band size: 80 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)

    Immunocytochemical analysis of, 4% paraformaldehyde-fixed at RT for 15 min, HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mre-11 (green) with ab214 at 1/200 dilution. Blue: Hoechst 33342 staining. Scale bar= 10 μm.

  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214), expandable thumbnail

    Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)

    This image was generated using the ascites version of the product.

    7.5% SDS-PAGE

    All lanes: Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214) at 1/1000 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Lane 2: Human Mre-11-transfected HEK-293T whole cell lysate at 30 µg

    Secondary

    All lanes: anti-mouse IgG HRP-conjugated antibody

    Predicted band size: 80 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214), expandable thumbnail
    Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.

    Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)

    Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
    (Panel D - negative control).

    This image was generated using the ascites version of the product.

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Product protocols

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