Anti-Mre11 antibody - ChIP Grade [EPR21027] (ab208020)

Rabbit Recombinant Monoclonal MRE11 antibody. Suitable for IHC-P, ChIP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

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Images

Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (AB208020), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ChIP	WB	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Expected	Expected
Rat	Tested	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes 5μg

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information MRE11

Function

Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:11741547, PubMed:14657032, PubMed:22078559, PubMed:23080121, PubMed:24316220, PubMed:26240375, PubMed:27889449, PubMed:28867292, PubMed:29670289, PubMed:30464262, PubMed:30612738, PubMed:31353207, PubMed:37696958, PubMed:38128537, PubMed:9590181, PubMed:9651580, PubMed:9705271). The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases (PubMed:24316220, PubMed:28867292, PubMed:31353207, PubMed:38128537). The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage (PubMed:24316220, PubMed:27889449, PubMed:28867292, PubMed:36050397, PubMed:38128537). Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity (PubMed:11741547, PubMed:22078559, PubMed:24316220, PubMed:26240375, PubMed:27889449, PubMed:29670289, PubMed:31353207, PubMed:36563124, PubMed:9590181, PubMed:9651580, PubMed:9705271). After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with RBBP8/CtIP to initiate end resection (PubMed:27814491, PubMed:27889449, PubMed:30787182). MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR (PubMed:24316220). It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation to create entry sites for EXO1- and DNA2-mediated 5' to 3' long-range resection, which is required for single-strand invasion and recombination (PubMed:24316220, PubMed:28867292). RBBP8/CtIP specifically promotes the endonuclease activity of MRE11 to clear protein-DNA adducts and generate clean double-strand break ends (PubMed:27814491, PubMed:27889449, PubMed:30787182). The MRN complex is also required for DNA damage signaling via activation of the ATM and ATR kinases: the nuclease activity of MRE11 is not required to activate ATM and ATR (PubMed:14657032, PubMed:15064416, PubMed:15790808, PubMed:16622404). The MRN complex is also required for the processing of R-loops (PubMed:31537797). The MRN complex is involved in the activation of the cGAS-STING pathway induced by DNA damage during tumorigenesis: the MRN complex acts by displacing CGAS from nucleosome sequestration, thereby activating it (By similarity). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888). MRE11 contains two DNA-binding domains (DBDs), enabling it to bind both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA).

Alternative names

HNGS1, MRE11A, MRE11, Double-strand break repair protein MRE11, Meiotic recombination 11 homolog 1, Meiotic recombination 11 homolog A, MRE11 homolog 1, MRE11 homolog A

Recommended products

Rabbit Recombinant Monoclonal MRE11 antibody. Suitable for IHC-P, ChIP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR21027
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Mre11 protein also known as MRE11A serves as an integral part of the DNA repair machinery in cells. It forms a component of the MRE11/RAD50/NBS1 (MRN) complex which is essential for the detection and repair of DNA double-strand breaks (DSBs). The molecular weight of the Mre11 protein is approximately 81 kDa. It is broadly expressed in various human tissues highlighting its extensive role in maintaining genomic stability.

Biological function summary

This protein acts in the repair of DSBs by initiating homologous recombination and non-homologous end joining pathways. Together with the RAD50 and NBS1 proteins Mre11 forms the MRN complex which processes DNA ends and signals to other repair mechanisms. Additionally Mre11's exonuclease and endonuclease activities are important for the resection of DNA at break sites facilitating subsequent repair synthesis.

Pathways

Mre11 is instrumental in the DNA damage response and maintenance of genomic integrity. It operates within the ATM (ataxia-telangiectasia mutated) signaling pathway which activates upon DNA damage and regulates cell cycle checkpoints. Mre11 interacts with the ATM protein modifying the cellular response to DNA damage. The complex also collaborates closely with BRCA1 an important regulator of the repair process and associated with preventing breast cancer development.

Associated diseases and disorders

Mutations or dysfunction in the MRE11A gene can be linked to several conditions including ataxia-telangiectasia-like disorder (ATLD) and Nijmegen breakage syndrome (NBS). These disorders result from impaired DNA repair mechanisms leading to increased sensitivity to radiation and predisposition to cancer. The NBS1 protein as part of the MRN complex works closely with Mre11 in these conditions. Both disorders highlight the critical role of Mre11 in safeguarding genomic stability and preventing disease.

Product promise

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10 product images

Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
Blocking/Dilution: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020) at 1/1000 dilution
All lanes: Human fetal kidney tissue lysate at 10 µg
Secondary
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 80 kDa
Observed band size: 81 kDa
Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
Blocking/Dilution: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020) at 1/5000 dilution
Lane 1: K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg
Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 4: D3 (mouse embryo stem cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 80 kDa
Observed band size: 81 kDa
Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
Blocking/Dilution: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020) at 1/1000 dilution
Lane 1: C6 (rat glial tumor cell line) whole cell lysate at 10 µg
Lane 2: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 4: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
Lane 5: Mouse brain tissue lysate at 10 µg
Lane 6: Rat brain tissue lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 80 kDa
Observed band size: 81 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101), ready to use. Nuclear staining in human colon tissue is observed (PMID: 25447316). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101), ready to use.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
ChIP - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
Chromatin was prepared from non-treated HeLa cells or HeLa cells treated with 1% Methyl methanesulfonate for 1 hour according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 5 μg of ab208020 (blue), and 20 μL of protein A/G sepharose beads slurry (10 μL of sepharose A beads + 10 μL of sepharose G beads). 5 μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101), ready to use. Nuclear staining in human endometrial carcinoma is observed (PMID: 24927325). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101), ready to use.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101), ready to use. Nuclear staining in mouse testis is observed (PMID: 10508516). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101), ready to use.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101), ready to use. Nuclear staining in rat colon tissue is observed (PMID: 28357369). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101), ready to use.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized K562 (Human human chronic myelogenous leukemia cell line from bone marrow) cell line labeling Mre11 with ab208020 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880) Ready to use. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880)
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Positive staining on human breast carcinoma. The section was incubated with ab208020 at 4°C overnight.