Anti-Mre11 antibody [EPR21027] - ChIP Grade
- RabMAb
- Recombinant
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(8 Publications)
Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020) is a rabbit monoclonal antibody detecting Mre11 in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ChIP. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
View Alternative Names
HNGS1, MRE11A, MRE11, Double-strand break repair protein MRE11, Meiotic recombination 11 homolog 1, Meiotic recombination 11 homolog A, MRE11 homolog 1, MRE11 homolog A
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (AB208020)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized K562 (Human human chronic myelogenous leukemia cell line from bone marrow) cell line labeling Mre11 with ab208020 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (AB208020)
Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use. Nuclear staining in human endometrial carcinoma is observed (PMID : 24927325). Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (AB208020)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) Ready to use. Counter stained with Hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) Positive staining on human breast carcinoma. The section was incubated with ab208020 at 4°C overnight.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (AB208020)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use. Nuclear staining in human colon tissue is observed (PMID : 25447316). Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
- ChIP
Supplier Data
ChIP - Anti-Mre11 antibody [EPR21027] - ChIP Grade (AB208020)
Chromatin was prepared from non-treated HeLa cells or HeLa cells treated with 1% Methyl methanesulfonate for 1 hour according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 5 μg of ab208020 (blue), and 20 μL of protein A/G sepharose beads slurry (10 μL of sepharose A beads + 10 μL of sepharose G beads). 5 μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (AB208020)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use. Nuclear staining in rat colon tissue is observed (PMID : 28357369). Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (AB208020)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use. Nuclear staining in mouse testis is observed (PMID : 10508516). Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (AB208020)
Blocking/Dilution : 5% NFDM/TBST.
Exposure time : 3 minutes.
All lanes:
Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020) at 1/1000 dilution
All lanes:
Human fetal kidney tissue lysate at 10 µg
Secondary
All lanes:
Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution
Predicted band size: 80 kDa
Observed band size: 81 kDa
true
- WB
Supplier Data
Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (AB208020)
Blocking/Dilution : 5% NFDM/TBST.
Exposure time : 3 minutes.
All lanes:
Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020) at 1/5000 dilution
Lane 1:
K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg
Lane 2:
Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 3:
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 4:
D3 (mouse embryo stem cell) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 80 kDa
Observed band size: 81 kDa
false
- WB
Supplier Data
Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (AB208020)
Blocking/Dilution : 5% NFDM/TBST.
Exposure time : 3 minutes.
All lanes:
Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020) at 1/1000 dilution
Lane 1:
C6 (rat glial tumor cell line) whole cell lysate at 10 µg
Lane 2:
RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 3:
PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 4:
NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
Lane 5:
Mouse brain tissue lysate at 10 µg
Lane 6:
Rat brain tissue lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 80 kDa
Observed band size: 81 kDa
false
Related conjugates and formulations (1)
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Anti-Mre11 antibody [EPR21027] - BSA and Azide free
Reactivity data
Product details
What is this antibody validated in?
Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ChIP in Human, Mouse, Rat samples.
What is the molecular weight of Mre11?
Anti-Mre11 [EPR21027] - ChIP Grade (ab208020) specifically detects a band for Mre11 (UniProt: P49959) at a molecular weight of 81kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR21027] also available for your convenience: ab208020, Carrier free - ab230381
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
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Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein acts in the repair of DSBs by initiating homologous recombination and non-homologous end joining pathways. Together with the RAD50 and NBS1 proteins Mre11 forms the MRN complex which processes DNA ends and signals to other repair mechanisms. Additionally Mre11's exonuclease and endonuclease activities are important for the resection of DNA at break sites facilitating subsequent repair synthesis.
Pathways
Mre11 is instrumental in the DNA damage response and maintenance of genomic integrity. It operates within the ATM (ataxia-telangiectasia mutated) signaling pathway which activates upon DNA damage and regulates cell cycle checkpoints. Mre11 interacts with the ATM protein modifying the cellular response to DNA damage. The complex also collaborates closely with BRCA1 an important regulator of the repair process and associated with preventing breast cancer development.
Product protocols
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Target data
Publications (8)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 15:1446 PubMed38365788
2024
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Nature communications 14:7430 PubMed37973845
2023
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Nucleic acids research 51:12207-12223 PubMed37897354
2023
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Nucleic acids research 51:10059-10074 PubMed37678882
2023
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2100779 PubMed34363353
2021
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Drug design, development and therapy 15:1749-1763 PubMed33953544
2021
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Oncology reports 44:2152-2164 PubMed32901871
2020
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Nature protocols 15:1775-1799 PubMed32313254
2020
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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