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AB183663

Anti-MRG15 antibody

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(2 Publications)

Rabbit Polyclonal MRG15 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MORF4L1 aa 50-350.

View Alternative Names

MRG15, FWP006, HSPC008, HSPC061, PP368, MORF4L1, Mortality factor 4-like protein 1, MORF-related gene 15 protein, Protein MSL3-1, Transcription factor-like protein MRG15

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MRG15 antibody (AB183663)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRG15 antibody (AB183663)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling MRG15 with ab183663 at 1/1000 dilution (green). The slide on the right is counterstained with Hoechst 33342 (blue).

Western blot - Anti-MRG15 antibody (AB183663)
  • WB

Supplier Data

Western blot - Anti-MRG15 antibody (AB183663)

10 % SDS-PAGE

All lanes:

Western blot - Anti-MRG15 antibody (ab183663) at 1/500 dilution

Lane 1:

Huh7 whole cell lysate/extract at 30 µg

Lane 2:

Hep3B whole cell lysate/extract at 30 µg

Predicted band size: 41 kDa

false

Western blot - Anti-MRG15 antibody (AB183663)
  • WB

Supplier Data

Western blot - Anti-MRG15 antibody (AB183663)

10 % SDS-PAGE

All lanes:

Western blot - Anti-MRG15 antibody (ab183663) at 1/500 dilution

All lanes:

Mouse liver lysate/extract at 50 µg

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MORF4L1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q9UBU8

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRG15 also known as MORF Related Gene 15 is a protein weighing approximately 34 kDa. This protein plays a significant role in chromatin remodeling and gene expression. Researchers have identified MRG15 in various tissues with a notable expression in neural and skeletal muscle tissues. The protein engages actively in the cellular processes related to chromatin and gene regulation which are essential for maintaining normal cellular function.
Biological function summary

MRG15 integrates into several protein complexes including the histone acetyltransferase complex where it interacts with other components to regulate transcription. The structure of MRG15 enables it to bind to methylated histones facilitating histone modification and affecting gene expression levels. Researchers observe its impact on the modulation of DNA repair and replication through its participation in these complexes.

Pathways

MRG15 operates mostly within the DNA damage repair and cell cycle progression pathways. It forms critical interactions with proteins such as ASF1 and TIP60 which influence histone acetylation and chromatin structure. These tasks are essential for the correct execution of the repair mechanisms and maintenance of genomic stability.

Researchers associate MRG15 with neurodegenerative diseases and certain cancer types. The protein's close relationship with BRCA1 and p300 which are also implicated in these conditions indicates its participation in tumorigenesis and neural degeneration processes. Dysfunctional MRG15 activity can lead to impaired DNA repair contributing to disease pathogenesis in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. As part of the SIN3B complex represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2 (PubMed : 12391155, PubMed : 14966270, PubMed : 37137925). SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription (PubMed : 21041482). Required for homologous recombination repair (HRR) and resistance to mitomycin C (MMC). Involved in the localization of PALB2, BRCA2 and RAD51, but not BRCA1, to DNA-damage foci.
See full target information MORF4L1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular biology 436:168414 PubMed38141874

2023

The Tudor-knot Domain of KAT5 Regulates Nucleosomal Substrate Acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Xuan,Hongwen Xuan,Mengying Huang,Wei He,Han Xu,Xiaobing Shi,Hong Wen

eLife 6: PubMed28639940

2017

Genetic specification of left-right asymmetry in the diaphragm muscles and their motor innervation.

Applications

WB

Species

Mouse

Camille Charoy,Sarah Dinvaut,Yohan Chaix,Laurette Morlé,Isabelle Sanyas,Muriel Bozon,Karine Kindbeiter,Bénédicte Durand,Jennifer M Skidmore,Lies De Groef,Motoaki Seki,Lieve Moons,Christiana Ruhrberg,James F Martin,Donna M Martin,Julien Falk,Valerie Castellani
View all publications
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Product promise

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