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AB254756

Anti-MRGPRF antibody

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Rabbit Polyclonal MRGPRF antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MRGPRF aa 1-50.

View Alternative Names

GPR140, GPR168, MRGF, PSEC0142, MRGPRF, Mas-related G-protein coupled receptor member F, Mas-related gene F protein, G-protein coupled receptor 140, G-protein coupled receptor 168

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGPRF antibody (AB254756)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGPRF antibody (AB254756)

Formalin-fixed, paraffin-embedded human endometrium tissue stained for MRGPRF with ab254756 at a 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-MRGPRF antibody (AB254756)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRGPRF antibody (AB254756)

PFA fixed, Triton X-100 permeabilized BJ (Human cell line) cells labeling MRGPRF using ab254756 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGPRF antibody (AB254756)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGPRF antibody (AB254756)

Formalin-fixed, paraffin-embedded human prostate tissue stained for MRGPRF with ab254756 at a 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGPRF antibody (AB254756)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGPRF antibody (AB254756)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue shows absence of immunoreactivity for MRGPRF when incubated with ab254756 at a 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGPRF antibody (AB254756)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGPRF antibody (AB254756)

Formalin-fixed, paraffin-embedded human kidney tissue stained for MRGPRF with ab254756 at a 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-MRGPRF antibody (AB254756)
  • WB

Supplier Data

Western blot - Anti-MRGPRF antibody (AB254756)

All lanes:

Western blot - Anti-MRGPRF antibody (ab254756) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T cell lysate

Lane 2:

MRGPRF overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MRGPRF aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q96AM1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRGPRF also known as Mas-related G-protein coupled receptor member F is a protein with an approximate mass of 37 kDa. It acts mechanically by binding to specific ligands and transducing signals through G-proteins which then trigger intracellular responses. Scientists identified its expression in several tissues including sensory neurons skin and smooth muscle cells. Its function as a receptor suggests its involvement in modulating various physiological processes.
Biological function summary

MRGPRF contributes to the modulation of pain and is part of the sensory perception of itch. It does not form a complex but operates potentially with other G-protein coupled receptors to mediate responses to environmental stimuli. Activation of MRGPRF may influence the release of neural peptides contributing to cellular communication within the nervous system. Its presence in skin also hints at roles in cutaneous sensory perception.

Pathways

MRGPRF interacts in the neuropeptide signaling and the inflammatory pathways. These pathways involve vital forms of cellular communication that play a role in reacting to external stimuli such as noxious chemicals. MRGPRF shows relationships with other proteins such as those belonging to the Mas-related gene family all of which participate in similar neuro-sensory responses. Its regulatory effects on inflammation suggest interactions with inflammatory mediators through these pathways.

MRGPRF could relate to chronic pain disorders and inflammation-driven skin diseases like atopic dermatitis. Changes in its function or expression may influence these conditions by altering sensory signaling. MRGPRF's role in these disorders connects with proteins involved in inflammatory responses such as cytokines which modulate immune system activity. Understanding MRGPRF's involvement in these diseases could offer insights into developing new therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Orphan receptor. May bind to a neuropeptide and may regulate nociceptor function and/or development, including the sensation or modulation of pain (By similarity).
See full target information MRGPRF
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Product promise

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