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AB250034

Anti-MRGX antibody [EPR9775(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MRGX antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0026, MRGX, MORF4L2, Mortality factor 4-like protein 2, MORF-related gene X protein, Protein MSL3-2, Transcription factor-like protein MRGX

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] - BSA and Azide free (AB250034)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] - BSA and Azide free (AB250034)

This data was developed using ab178405, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian carcinoma tissue labeling MRGX with ab178405 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] - BSA and Azide free (AB250034)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] - BSA and Azide free (AB250034)

This data was developed using ab178405, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling MRGX with ab178405 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] - BSA and Azide free (AB250034)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] - BSA and Azide free (AB250034)

This data was developed using ab178405, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells labeling MRGX with ab178405 at 1/50 dilution.

Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] - BSA and Azide free (AB250034)
  • IP

Supplier Data

Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] - BSA and Azide free (AB250034)

This data was developed using ab178405, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling MRGX immunoprecipitated using ab178405 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (<a href='/en-us/products/primary-antibodies/mrgx-antibody-epr97752-ab178405'>ab178405</a>)

Predicted band size: 32 kDa

true

Western blot - Anti-MRGX antibody [EPR9775(2)] - BSA and Azide free (AB250034)
  • WB

Supplier Data

Western blot - Anti-MRGX antibody [EPR9775(2)] - BSA and Azide free (AB250034)

This data was developed using ab178405, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRGX antibody [EPR9775(2)] (<a href='/en-us/products/primary-antibodies/mrgx-antibody-epr97752-ab178405'>ab178405</a>) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

U-87 MG cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP-conjugated at 1/2000 dilution

Predicted band size: 32 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9775(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250034 is the carrier-free version of ab178405.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRGX also known as Mas-related G protein-coupled receptor member X functions as a G protein-coupled receptor (GPCR) involved in sensing environmental stimuli and transducing signals inside cells. Researchers find this receptor in sensory neurons within the dorsal root ganglia and trigeminal ganglia. MRGX weighs approximately 37 kDa and plays a role in pain perception and modulation by interacting with various ligands. Understanding its mechanical function helps in exploring its wide applications in sensory biology.
Biological function summary

This receptor contributes to the modulation of nociceptive signals and influences pain sensation and response. MRGX does not form part of a complex; it functions independently to modulate pain pathways by interacting with peptides and other small molecules. It also regulates calcium ion concentrations within neurons which is essential for neurotransmission and signal propagation. The interaction of MRGX with these elements highlights its significant contribution to the sensory regulatory processes.

Pathways

Research associates MRGX with pain perception and sensory transmission pathways. It intersects with the neuropeptide signaling pathway influencing proteins such as substance P and calcitonin gene-related peptide (CGRP) both key players in pain signal pathways. MRGX modulates the activity of these signaling molecules affecting how the body interprets and reacts to painful stimuli. This receptor's involvement in these pathways demonstrates its impact on the physiological responses to pain.

MRGX is linked to conditions such as neuropathic pain and inflammatory pain. Alterations in MRGX function or expression can influence the severity of pain-related phenomena implicating it in these conditions. The association of MRGX with proteins like TRPV1 and Nav1.8 which are involved in pain pathways further solidifies its connection to these disorders. Understanding these interactions offers insights into potential therapeutic avenues for treating chronic pain syndromes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also a component of the MSIN3A complex which acts to repress transcription by deacetylation of nucleosomal histones.
See full target information MORF4L2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:65 PubMed39972431

2025

Mechanism of histone demethylase KDM5A in osteoporotic fracture healing through epigenetic regulation of the miR-495/SKP2/Runx2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoran Li,Junyan Zhang,Tingting Xu,Zhiying Hao,Yadong Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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