Rabbit Polyclonal MRI antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CYREN aa 1 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CYREN aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BWK5
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
Target data
Function
Cell-cycle-specific regulator of classical non-homologous end joining (NHEJ) of DNA double-strand break (DSB) repair, which can act both as an activator or inhibitor of NHEJ, depending on the cell cycle phase (PubMed:24610814, PubMed:28959974). Acts as a regulator of DNA repair pathway choice by specifically inhibiting classical NHEJ during the S and G2 phases, thereby promoting error-free repair by homologous recombination during cell cycle phases when sister chromatids are present (PubMed:28959974). Preferentially protects single-stranded overhangs at break sites by inhibiting classical NHEJ, thereby creating a local environment that favors homologous recombination (PubMed:28959974). Acts via interaction with XRCC5/Ku80 and XRCC6/Ku70 (PubMed:28959974). In contrast, acts as an activator of NHEJ during G1 phase of the cell cycle: promotes classical NHEJ in G1 phase cells via multivalent interactions that increase the affinity of DNA damage response proteins for DSB-associated chromatin. Also involved in immunoglobulin V(D)J recombination (By similarity). May also act as an indirect regulator of proteasome (By similarity).
Alternative names
C7orf49, MRI, CYREN, Cell cycle regulator of non-homologous end joining, Cell cycle regulator of NHEJ, Modulator of retrovirus infection homolog
Recommended products
Rabbit Polyclonal MRI antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CYREN aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CYREN aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BWK5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Magnetic Resonance Imaging (MRI) is not a protein or biological target in the traditional sense but is a powerful imaging technique used in the life sciences. MRI functions mechanically by generating strong magnetic fields and radio waves to produce detailed images of organs and tissues. It does not express any mass because it is an instrument not a biomolecule. MRI is widely used across many fields and is essential in hospitals and research settings. Despite not being a protein MRI relies on the interaction with hydrogen protons particularly prevalent in water and fat to provide high-resolution images of soft tissues.
- Biological function summary
MRI provides critical insights into the structure and function of the human body by visualizing soft tissues in great detail. This technology plays a powerful role in identifying the complex anatomy of tissues and organs giving it a significant presence in medical diagnostics. MRI can assess functional aspects of biological processes through techniques like functional MRI (fMRI) which measures brain activity by detecting changes in blood flow. Though MRI is not a biological molecule or part of any specific complex it contributes substantially to biological research by helping understand tissue composition and organ dysfunctions.
- Pathways
MRI significantly impacts the study of biological processes and pathways by allowing detailed observation of real-time physiological processes. MRI is indispensable in evaluating the pathways related to neurological and cardiovascular systems. For example it aids in the visualization of neural connections and the assessment of blood vessels offering insights into the dynamic processes within these networks. Despite not being directly involved with molecular pathways MRI complements studies involving proteins such as those implicated in neural signaling or cardiac muscle contractions by enabling precise structural and functional assessments.
- Associated diseases and disorders
MRI offers invaluable aid in diagnosing and monitoring various conditions including brain disorders and cardiovascular diseases. MRI’s high-resolution imaging helps identify abnormalities like tumors lesions or ischemic areas with precision often involving proteins like amyloid beta in Alzheimer's disease or troponin in conditions related to the heart. By visualizing disease progression or treatment impact MRI supports the understanding and management of these disorders making it an important tool in clinical practice and research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-MRI antibody (ab246926)
PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling MRI using ab246926 at 4 μg/ml (green) in ICC/IF.
Western blot - Anti-MRI antibody (ab246926)
All lanes: Western blot - Anti-MRI antibody (ab246926) at 0.4 µg/mL
Lane 1: Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2: MRI overexpression HEK-293T lysate
Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRI antibody (ab246926)
Formalin-fixed, paraffin-embedded human duodenum tissue stained for MRI with ab246926 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRI antibody (ab246926)
Formalin-fixed, paraffin-embedded human prostate tissue stained for MRI with ab246926 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRI antibody (ab246926)
Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for MRI with ab246926 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRI antibody (ab246926)
Formalin-fixed, paraffin-embedded human pancreas tissue shows absence of immunoreactivity for MRI when incubated with ab246296 at a 1/50 dilution (negative control).
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