JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB181048

Anti-MRO antibody [EPR12158]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal MRO antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 2 publications.

View Alternative Names

B29, C18orf3, MRO, Protein maestro, Male-specific transcription in the developing reproductive organs, Protein B29

6 Images
Immunoprecipitation - Anti-MRO antibody [EPR12158] (AB181048)
  • IP

Unknown

Immunoprecipitation - Anti-MRO antibody [EPR12158] (AB181048)

ab181048 (purified) at 1/20 immunoprecipitating MRO in 10 μg human fetal heart whole cell lysate (Lanes 1 and 2, observed at 29 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-MRO antibody [EPR12158] (ab181048)

Predicted band size: 29 kDa

false

Immunoprecipitation - Anti-MRO antibody [EPR12158] (AB181048)
  • IP

Supplier Data

Immunoprecipitation - Anti-MRO antibody [EPR12158] (AB181048)

Western blot analysis of immunoprecipitation pellet from Human fetal heart lysate (lane 1) or a negative control (lane 2) immunoprecipitated using unpurified ab181048 at 1/80 dilution.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-MRO antibody [EPR12158] (ab181048)

Predicted band size: 29 kDa

false

Western blot - Anti-MRO antibody [EPR12158] (AB181048)
  • WB

Lab

Western blot - Anti-MRO antibody [EPR12158] (AB181048)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MRO antibody [EPR12158] (ab181048) at 1/5000 dilution

All lanes:

human fetal heart lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 21 kDa,29 kDa

Observed band size: 21 kDa,29 kDa

false

Western blot - Anti-MRO antibody [EPR12158] (AB181048)
  • WB

Lab

Western blot - Anti-MRO antibody [EPR12158] (AB181048)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MRO antibody [EPR12158] (ab181048) at 1/5000 dilution

All lanes:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Western blot - Anti-MRO antibody [EPR12158] (AB181048)
  • WB

Supplier Data

Western blot - Anti-MRO antibody [EPR12158] (AB181048)

All lanes:

Western blot - Anti-MRO antibody [EPR12158] (ab181048) at 1/1000 dilution

Lane 1:

Human ovary cancer lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 29 kDa

false

Western blot - Anti-MRO antibody [EPR12158] (AB181048)
  • WB

Lab

Western blot - Anti-MRO antibody [EPR12158] (AB181048)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MRO antibody [EPR12158] (ab181048) at 1/5000 dilution

Lane 1:

mouse spleen lysate at 10 µg

Lane 2:

rat spleen lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12158

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB181058

Anti-MRPL20 antibody [EPR12592]

RabMAb|Recombinant

Western blot - Anti-MRPL20 antibody [EPR12592] (AB181058)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB181131

Anti-PTGR1 antibody [EPR13451-10]

RabMAb|Recombinant|KO Validated

Western blot - Anti-PTGR1 antibody [EPR13451-10] (AB181131)

  • 0
  • 0
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20 - 1/80", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/20 - 1/80", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRO also known as Maestro is a protein with a molecular mass of approximately 36 kDa. The MRO gene encodes this protein and it plays a role in transcriptional regulation. MRO shows expression mainly in testicular tissue suggesting its involvement in reproductive processes. Researchers detect its presence largely in the developing testes where it may influence the maturation and differentiation of germ cells.
Biological function summary

The MRO protein influences pathways involved with reproductive development. It associates with other proteins to form complexes that affect gene expression regulation in germ cells. MRO has been identified as a factor in sex determination and differentiation. It interacts with various transcription factors to modulate the activity of genes essential for the formation and maintenance of spermatogonial stem cells.

Pathways

The MRO protein integrates into the sex determination and reproductive development pathways. It has ties to the WNT signaling pathway an important pathway for developmental processes. In these pathways MRO interacts with key proteins like SOX9 a critical factor in testis formation and DMRT1 which is involved in sex differentiation. This interaction network allows MRO to influence the development of the male reproductive organs.

MRO relates closely to infertility and disorders of sexual development. Researchers have linked aberrant expression of the MRO protein to issues in spermatogenesis which can lead to male infertility. Additionally MRO's interactions with other proteins such as NR5A1 implicated in disorders of sexual development highlight its potential role in these conditions. Through these associations MRO represents a notable target for understanding and potentially mitigating male reproductive health issues.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information MRO
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:920 PubMed40817243

2025

MRO/HNRNPU/CCL5 feedback loop amplifies M2 macrophage and breast cancer cell crosstalk to drive progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhirui Zeng,Xin Bao,Shan Lei,Wenjing Tang,Huading Tai,Lu Wang,Dahuan Li,Shu Liu,Tengxiang Chen

PloS one 12:e0174873 PubMed28406912

2017

The elusive MAESTRO gene: Its human reproductive tissue-specific expression pattern.

Applications

WB

Species

Human

Shlomit Kenigsberg,Patricia D A Lima,Leila Maghen,Brandon A Wyse,Chantal Lackan,Annie N Y Cheung,Benjamin K Tsang,Clifford L Librach
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com