Skip to main content

Rabbit Recombinant Monoclonal MRO antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

Be the first to review this product! Submit a review

Images

Western blot - Anti-MRO antibody [EPR12158] (AB181048), expandable thumbnail
  • Western blot - Anti-MRO antibody [EPR12158] (AB181048), expandable thumbnail
  • Immunoprecipitation - Anti-MRO antibody [EPR12158] (AB181048), expandable thumbnail
  • Western blot - Anti-MRO antibody [EPR12158] (AB181048), expandable thumbnail
  • Immunoprecipitation - Anti-MRO antibody [EPR12158] (AB181048), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/20 - 1/80
Notes

-

Expected
Expected

Species
Rat
Dilution info
-
Notes

-

Species
Mouse
Dilution info
1/20 - 1/80
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

-

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/5000
Notes

-

Target data

Alternative names

B29, C18orf3, MRO, Protein maestro, Male-specific transcription in the developing reproductive organs, Protein B29

Recommended products

Rabbit Recombinant Monoclonal MRO antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR12158
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MRO also known as Maestro is a protein with a molecular mass of approximately 36 kDa. The MRO gene encodes this protein and it plays a role in transcriptional regulation. MRO shows expression mainly in testicular tissue suggesting its involvement in reproductive processes. Researchers detect its presence largely in the developing testes where it may influence the maturation and differentiation of germ cells.

Biological function summary

The MRO protein influences pathways involved with reproductive development. It associates with other proteins to form complexes that affect gene expression regulation in germ cells. MRO has been identified as a factor in sex determination and differentiation. It interacts with various transcription factors to modulate the activity of genes essential for the formation and maintenance of spermatogonial stem cells.

Pathways

The MRO protein integrates into the sex determination and reproductive development pathways. It has ties to the WNT signaling pathway an important pathway for developmental processes. In these pathways MRO interacts with key proteins like SOX9 a critical factor in testis formation and DMRT1 which is involved in sex differentiation. This interaction network allows MRO to influence the development of the male reproductive organs.

Associated diseases and disorders

MRO relates closely to infertility and disorders of sexual development. Researchers have linked aberrant expression of the MRO protein to issues in spermatogenesis which can lead to male infertility. Additionally MRO's interactions with other proteins such as NR5A1 implicated in disorders of sexual development highlight its potential role in these conditions. Through these associations MRO represents a notable target for understanding and potentially mitigating male reproductive health issues.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-MRO antibody [EPR12158] (ab181048), expandable thumbnail

    Western blot - Anti-MRO antibody [EPR12158] (ab181048)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-MRO antibody [EPR12158] (ab181048) at 1/5000 dilution

    Lane 1: mouse spleen lysate at 10 µg

    Lane 2: rat spleen lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

  • Western blot - Anti-MRO antibody [EPR12158] (ab181048), expandable thumbnail

    Western blot - Anti-MRO antibody [EPR12158] (ab181048)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-MRO antibody [EPR12158] (ab181048) at 1/5000 dilution

    All lanes: human fetal heart lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 21 kDa, 29 kDa

    Observed band size: 21 kDa, 29 kDa

  • Immunoprecipitation - Anti-MRO antibody [EPR12158] (ab181048), expandable thumbnail

    Immunoprecipitation - Anti-MRO antibody [EPR12158] (ab181048)

    ab181048 (purified) at 1/20 immunoprecipitating MRO in 10 μg human fetal heart whole cell lysate (Lanes 1 and 2, observed at 29 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-MRO antibody [EPR12158] (ab181048)

    Predicted band size: 29 kDa

  • Western blot - Anti-MRO antibody [EPR12158] (ab181048), expandable thumbnail

    Western blot - Anti-MRO antibody [EPR12158] (ab181048)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-MRO antibody [EPR12158] (ab181048) at 1/5000 dilution

    All lanes: Human fetal brain tissue lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

  • Immunoprecipitation - Anti-MRO antibody [EPR12158] (ab181048), expandable thumbnail

    Immunoprecipitation - Anti-MRO antibody [EPR12158] (ab181048)

    Western blot analysis of immunoprecipitation pellet from Human fetal heart lysate (lane 1) or a negative control (lane 2) immunoprecipitated using unpurified ab181048 at 1/80 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-MRO antibody [EPR12158] (ab181048)

    Predicted band size: 29 kDa

  • Western blot - Anti-MRO antibody [EPR12158] (ab181048), expandable thumbnail

    Western blot - Anti-MRO antibody [EPR12158] (ab181048)

    All lanes: Western blot - Anti-MRO antibody [EPR12158] (ab181048) at 1/1000 dilution

    Lane 1: Human ovary cancer lysate at 10 µg

    Lane 2: Human fetal brain lysate at 10 µg

    Lane 3: Human fetal heart lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution

    Predicted band size: 29 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com