Skip to main content

Rabbit Recombinant Monoclonal MRP1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

Be the first to review this product! Submit a review

Images

Western blot - Anti-MRP1 antibody [EPR21061] (AB230948), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (AB230948), expandable thumbnail
  • Western blot - Anti-MRP1 antibody [EPR21061] (AB230948), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (AB230948), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (AB230948), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Mediates export of organic anions and drugs from the cytoplasm (PubMed:10064732, PubMed:11114332, PubMed:16230346, PubMed:7961706, PubMed:9281595). Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics (PubMed:10064732, PubMed:11114332, PubMed:16230346, PubMed:7961706, PubMed:9281595). Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export (PubMed:9281595). Hydrolyzes ATP with low efficiency (PubMed:16230346). Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation (PubMed:17050692). Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthezing cells (By similarity). Mediates ATP-dependent, GSH-independent cyclic GMP-AMP (cGAMP) export (PubMed:36070769). Thus, by limiting intracellular cGAMP concentrations negatively regulates the cGAS-STING pathway (PubMed:36070769).

Alternative names

MRP, MRP1, ABCC1, Multidrug resistance-associated protein 1, ATP-binding cassette sub-family C member 1, Glutathione-S-conjugate-translocating ATPase ABCC1, Leukotriene C(4) transporter, LTC4 transporter

Recommended products

Rabbit Recombinant Monoclonal MRP1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21061
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MRP1 also known as ABCC1 is a transporter protein with a mass of approximately 190 kDa. This protein belongs to the ATP-binding cassette (ABC) transporter family. MRP1 actively exports a variety of substrates from cells by hydrolyzing ATP to ADP and inorganic phosphate. You can find MRP1 expressed in many tissues including the lung testis and kidney. It helps in cellular detoxification by exporting organic anions and other conjugated metabolites.

Biological function summary

This protein functions as an important determinant of multidrug resistance in cancer treatment. MRP1 can form part of a larger protein complex that includes other transporters like MRP2. Through its activity MRP1 protects tissues from toxic substances by transporting them out of the cell. Its role in transporting glutathione-conjugated compounds highlights its importance in cellular defense mechanisms against oxidative stress and xenobiotics.

Pathways

MRP1 participates in the glutathione metabolism and the xenobiotic efflux pathways. Both pathways involve cellular detoxification and the accumulation of anomalies can cause harmful effects in the body. MRP1 works with proteins such as GSTP1 which conjugates toxic substances with glutathione preparing them for export by MRP1. This coordination ensures efficient detoxification and protection of cells from damage.

Associated diseases and disorders

MRP1 has associations with several conditions particularly drug resistance in various cancers and chronic obstructive pulmonary disease (COPD). In cancer MRP1 overexpression often results in reduced treatment efficacy due to chemotherapy drugs being expelled from the cell helping to resist their cytotoxic effects. Research indicates a connection between MRP1 and P-glycoprotein (ABCB1) in cancer drug resistance pointing to a broader resistive mechanism in which multiple transporters are involved.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-MRP1 antibody [EPR21061] (ab230948), expandable thumbnail

    Western blot - Anti-MRP1 antibody [EPR21061] (ab230948)

    Lanes 1 - 4: Merged signal (red and green). Green - ab230948 observed at 170 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab230948 was shown to react with MRP1 in wild-type HeLa cells in Western blot with loss of signal observed in ABCC1 knockout cell line (ABCC1 knockout cell lysate). Wild-type HeLa and ABCC1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab230948 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-MRP1 antibody [EPR21061] (ab230948) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: ABCC1 knockout A549 cell lysate at 20 µg

    Lane 3: Wild-type HeLa cell lysate at 20 µg

    Lane 4: ABCC1 knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 171 kDa

    Observed band size: 170 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)

    Immunohistochemical analysis of paraffin-embedded human esophagus cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous staining in human esophageal cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:26870278) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-MRP1 antibody [EPR21061] (ab230948), expandable thumbnail

    Western blot - Anti-MRP1 antibody [EPR21061] (ab230948)

    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-MRP1 antibody [EPR21061] (ab230948) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

    Lane 2: A549 (human lung carcinoma epithelial cell) whole cell lysate at 10 µg

    Lane 3: BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate at 10 µg

    Lane 4: SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate at 10 µg

    Lane 5: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 171 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)

    Immunohistochemical analysis of paraffin-embedded human lung cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous and cytoplasmic staining in human lung cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:23667609) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)

    Immunohistochemical analysis of paraffin-embedded human colon cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous and cytoplasmic staining in human colon cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:23667609) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com